LOCUS       V036939                10873 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036939
VERSION     V036939
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4899
                     /label="Ablim1(NM_178688)"
                     /note="Ablim1(NM_178688)"
                     /gene="Ablim1"
     CDS             4906..4971
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5015..5226
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5239..5507
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5536..5545
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5566..6228
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6298..6351
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6352..6951
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6952..7540
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7614..7847
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7919..8040
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8080..8215
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8248..8264)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8272..8288
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8296..8326)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8341..8362
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8650..9238)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9409..10269)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10270..10374)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10848..10864
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc catccttgct tgggttaaag
     2341 tgtctgggga aattgtgcag ctctgagata ggcaaagttc cctcaccaga gagagccagc
     2401 ctcaggaact cccacaggag actgcttatt gaggacctga gtgtccccga gactcctgac
     2461 cctgctcatc gcaggcgtgg cacagtcata catctggtct atctgtattc tgctggctgt
     2521 ggtccaccag agctcaggtt tagcagctat gatccatccg tggcccatcc tcaggaccct
     2581 caccactcgt cggagaaacc tgtcattcac tgccataaat gcggggagcc atgcaagggc
     2641 gaagtgctac gggtgcagac caagcatttc cacatcaaat gcttcacctg caaagtgtgc
     2701 ggctgcgacc tggcccaggg gggcttcttc ataaagaacg gggactatct gtgtaccctg
     2761 gactaccaga ggatgtatgg gacacggtgc cacggctgtg gggagttcgt ggagggcgag
     2821 gtggtaactg ccctgggcaa gacctaccac cccaactgct tcgcctgtac aatctgcaag
     2881 cgcccatttc cacccggaga ccgagtcaca ttcaacggga gagactgtct ttgtcagctc
     2941 tgtgcacagc cgatgtcttc cagccctaaa gaagcctcct gctccagcaa ctgcgccggc
     3001 tgtggaaggg acattaagaa cggacaggcg ctgctagctc tggacaagca gtggcatttg
     3061 ggctgcttca agtgcaagtc ctgtgggaag gtcctcaccg gcgagtacat cagcaaggat
     3121 ggctccccat actgtgagaa agactaccag gggctctttg gggtgaagtg tgaagcctgt
     3181 caccagttta tcacaggaaa agtcctggag gcaggcgaca agcattatca tcccagctgc
     3241 gcacgatgca gcagatgcaa tcagatgttc acagaagggg aagaaatgta tctgcaaggt
     3301 tctaccgtgt ggcatcccga ctgtaagcag tccactaaga cagaggagaa gctgcggcca
     3361 ccaaacatcc cgcgctcttc atccgatttc ttttacccca aaagtctgat tcgtcgcaca
     3421 ggacggtccc cagctctgca gcttctgtcg ccaccctgtc tgatgaactc caacaaaaac
     3481 cccaggcagc ccaccaggac ctcctctgaa agtatctatt ctagaccagg ctccagcatc
     3541 cctggttcac caggccatac tatctatgca aaagtagaca atgaaatcct ggattacaag
     3601 gatttagcag ccatccccaa ggtcaaggcg atctatgaca tcgagcgtcc agatctcatt
     3661 acctatgagc ctttctacac atcaggctat gaggacaagc aggagagaca gagccttgga
     3721 gagtctccaa ggactttgtc tccaactcca tctgcagaag gttatcaaga tgtccgggat
     3781 cggatgatcc acaggtccac cagccagggc tccatcaact cccctgtgta tagccggcac
     3841 agttacactc caactacgtc tcgctcgccc cagcacttcc accgacctga gctcttgtct
     3901 cctggtgtgc acaggtggtc tcccctgcgc accagcagct tcagctccac ccacagcgac
     3961 tcccgtccca accccccctt ccgacaccac ttcctccccc atgtcaaagg caatgagccg
     4021 tccagcggcc ggaactcccc tctcccctac cggcccgaca gccgccctct aactccaact
     4081 tacgctcagg cccctaaaca tttccatgtt ccagatcaag ggatcaacat ttaccgaaaa
     4141 ccacccatct acaaacagca tgctgccttg gcagcccaga gcaaggcttc agaagatatc
     4201 atcaagtttt ccaagttccc agcagcccag gcgccagatc ccaacgagat accaaagatt
     4261 gagacggacc actggccggg tcccccctca cttgccgccg taggaactga cccgaggcgc
     4321 agatcaagtg gcagagagga agatgaagag gagcttttga gacgccggca gcttcaagaa
     4381 gaacagttga tgaagctcaa ctcaggcctg gggcagctga tcctgaagga agagatggag
     4441 aaggagagca gagaaagagc atcgctggcc agtcgctatg actctcccct ccactcagct
     4501 tcccatgctc catcatctaa aacttcatct ctccctggtt atggaaagaa tggccttcac
     4561 cggccagttt ccacagactt cgctcagtac aacagctacg gagacatcag tggcggagtt
     4621 cgagactacc agaccctccc agatggccac atgcctgcag tgagaatgga cagaggagta
     4681 tccatgccca acatgttgga accaaagata tttccatatg agatgctcat ggtgaccaac
     4741 agagggcgca acaaaatcct gagagatgtg gatagaaccc ggctagagcg ccacttagcc
     4801 ccagaagtat tttgggagat ctttgggatg tccatacagg aatttgacaa gttacctctt
     4861 tggagacgca acgacatgaa gaaaaaagct aaactcttcg gatccgacta caaagaccat
     4921 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
     4981 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     5041 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     5101 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     5161 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     5221 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     5281 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     5341 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     5401 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     5461 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     5521 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc
     5581 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
     5641 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
     5701 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
     5761 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc
     5821 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag
     5881 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg
     5941 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc
     6001 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac
     6061 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag
     6121 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg
     6181 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct
     6241 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag
     6301 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag
     6361 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     6421 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     6481 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     6541 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     6601 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     6661 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     6721 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     6781 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     6841 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     6901 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct
     6961 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     7021 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     7081 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     7141 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     7201 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7261 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7321 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     7381 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7441 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7501 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
     7561 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7621 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt
     7681 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7741 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7801 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7861 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     7921 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7981 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8041 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     8101 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8161 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     8221 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     8281 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8341 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8401 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8461 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8521 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8581 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8641 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8701 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8761 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8821 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8881 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8941 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9001 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9061 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9121 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9181 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9241 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9301 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9361 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9421 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9481 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9541 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9601 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9661 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9721 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9781 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9841 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9901 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9961 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10021 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10081 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10141 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10201 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10261 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10321 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10381 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    10441 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    10501 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    10561 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    10621 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    10681 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    10741 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    10801 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    10861 cagtgccaag ctg
//
