LOCUS V040432 15003 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V040432 VERSION V040432 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2303..9085 /label="Abca1(NM_013454)" /note="Abca1(NM_013454)" /gene="Abca1" CDS 9092..9157 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 9201..9412 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 9425..9693 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 9728..10435 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 10445..10498 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 10499..11098 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 11099..11686 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 11760..11993 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 12065..12186 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 12205..12340 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(12378..12394) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 12402..12418 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(12426..12456) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 12471..12492 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(12780..13368) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(13539..14399) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(14400..14504) /gene="bla" /label="AmpR promoter" primer_bind 14978..14994 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggcttg ttggcctcag ttaaggctgc tgctgtggaa 2341 gaatctgaca tttcgaagga gacaaacatg tcagctgtta ctggaagtgg cctggcctct 2401 ctttatcttc ctgatcctga tctctgtacg cctgagctac ccaccctacg aacaacatga 2461 gtgccacttt ccgaataaag ccatgccgtc tgcaggaacc ctcccctggg tacaggggat 2521 tatctgtaat gccaacaacc cctgcttccg ttatccaact cccggcgagg ctcccggtgt 2581 tgttggaaac tttaacaagt ccatcgtgtc tcgcctgttc tcagacgctc agaggcttct 2641 tctgtacagc caaagagata ccagcattaa ggacatgcac aaggtcctga gaatgttacg 2701 gcagatcaag catcccaact caaatttgaa gctccaggat tttctggtgg acaatgaaac 2761 attctctgga ttcctgcagc acaatttgtc ccttccaaga tctactgtgg acagcctgct 2821 gcaggcgaat gttggtctcc agaaggtatt tttgcaaggc taccaattac atttggccag 2881 tctgtgtaac ggatcaaaat tagaagaaat tattcagctt ggtgatgcgg aagtttctgc 2941 cctctgtggt ctaccgagga agaagctcga tgcagccgag agagtactgc gctacaacat 3001 ggacatcctg aagccagttg tgacaaaact aaattccaca tctcatctcc cgacccagca 3061 tctggctgaa gccaccacag tgttgcttga cagcttgggg ggcctggccc aagagctgtt 3121 cagcacaaag agctggagcg acatgcggca ggaggtgatg tttctgacca acgtgaacag 3181 ctccagctcc tccacccaga tctaccaggc agtgtcccgc atcgtgtgtg gtcacccaga 3241 gggtgggggc ctgaagatca agtccctcaa ctggtacgag gataacaact acaaagccct 3301 ctttggaggg aataacaccg aggaagacgt ggacaccttc tatgacaatt ctacaactcc 3361 ttattgcaat gatttgatga agaacttgga gtctagtcct ctttctcgaa ttatttggaa 3421 ggcactcaag ccactgcttg ttggaaagat tctctataca cctgacacac cagctacaag 3481 gcaggtcatg gctgaggtga acaagacctt tcaggagttg gctgtgttcc atgacctgga 3541 gggcatgtgg gaagaactca gcccccaaat ttggaccttc atggagaaca gccaagagat 3601 ggaccttgtc cggacgctgt tagacagcag aggcaatgac cagttttggg aacagaagtt 3661 ggatggatta gattggactg cccaagacat catggcgttt ctggccaaga acccagaaga 3721 tgttcagtcc ccaaatggct ctgtgtatac ctggagagaa gctttcaatg agaccaacca 3781 ggcaatccag acgatatctc gattcatgga gtgtgtcaac ctgaacaagc tggaacccat 3841 tccgacagaa gtcaggctca tcaacaagtc catggagctg ctggacgaga ggaagttctg 3901 ggctggcatc gtgttcacag gcatcactcc agatagtgtg gagctgcccc atcatgtaaa 3961 gtacaagatc cggatggaca ttgacaacgt ggagagaact aataagatca aggatgggta 4021 ctgggaccct ggtcctcggg ctgacccttt tgaagatatg cgctatgtct ggggcggctt 4081 cgcctacttg caggatgtgg tggaacaggc catcatcaga gtgctgacgg gatctgagaa 4141 gaaaacgggt gtctacgtgc aacagatgcc ctacccctgt tatgttgatg acatttttct 4201 gcgggtcatg agccggtcaa tgcccctctt catgactcta gcctggatct actctgtcgc 4261 tgtgatcatc aagagcattg tgtatgagaa ggaggctcgg ctgaaggaga ccatgcggat 4321 catgggtctg gacaatggca tcctctggtt tagctggttt gttagcagcc tcatccctct 4381 gcttgtgagc gctggcctgc tggtggtcat cttgaagtta ggaaacctgc tgccctatag 4441 tgaccccagc gtggtgttcg tcttcctgtc tgtgtttgcc atggtgacca tcctacagtg 4501 cttcctcatt agcacgctct tctcccgtgc caacctggca gcagcctgtg ggggcatcat 4561 ctacttcacg ctgtacctgc cctatgtgct gtgcgtagcc tggcaggact atgtgggctt 4621 ctccatcaag atctttgcta gcctgctgtc tcctgtggct tttggattcg gctgtgagta 4681 tttcgccctt ttcgaggagc aaggtatcgg ggtccaatgg gacaatctct ttgagagccc 4741 ggtggaggag gacggcttca atctcaccac tgcagtgtcc atgatgctct ttgacacctt 4801 tctctatggc gtgatgacat ggtacatcga agccgtcttt ccaggacagt atggaattcc 4861 caggccctgg tattttcctt gtaccaagtc atactggttt ggtgaggaaa ttgatgagaa 4921 gagccaccct ggttccagcc agaagggagt gtcagaaatc tgcatggaag aggaacccac 4981 tcatctgagg ctgggggtgt ccattcagaa cctggtgaag gtttaccgag atggcatgaa 5041 ggttgctgtg gatggcttgg cgctcaactt ttacgaaggc cagattacct ccttcctggg 5101 ccacaatgga gcagggaaga ccaccaccat gtcaatactg actgggctgt ttcccccaac 5161 ttctggcacg gcctacatcc tggggaagga cattcgctcg gagatgagct ccatccggca 5221 gaacctggga gtctgtcccc agcataatgt gctgtttgac atgctgactg tcgaagagca 5281 catctggttc tatgcgcgcc taaaggggct ctcagagaag cacgtgaaag cagagatgga 5341 gcagatggcc ctggatgttg gcttaccccc gagcaagctg aaaagcaaaa cgagtcagct 5401 ctcaggtggg atgcagagaa agctgtctgt ggccttggcc ttcgtgggtg gatccaaggt 5461 tgtcattctg gacgagccca cagccggggt ggacccgtac tctcgcaggg gaatatggga 5521 actcctgcta aaataccggc aaggccgcac cattattttg tctacacacc acatggacga 5581 agctgacatc cttggggaca gaattgccat catttcccat gggaagctgt gttgtgtggg 5641 ctcctccctg tttttgaaaa accagttggg aacgggttac tatctgaccc tggttaagaa 5701 agatgtggaa tcgtccctca gttcctgcag aaacagtagc agcaccgtgt cttgtctgaa 5761 aaaggaggac agtgtttctc agagcagttc tgatgctggc ctgggcagcg accatgaaag 5821 tgacacgctg accatcgatg tctctgctat ctccaacctc atcaggaagc acgtgtctga 5881 agcccggctg gtggaggaca ttgggcacga gctgacctat gtgctgccgt acgaagccgc 5941 gaaggaggga gcctttgtgg aactcttcca tgagattgat gaccggctct cagacctggg 6001 catctccagt tatggcatct cggagaccac cctggaagaa atattcctca aagtggctga 6061 agagagcggg gtggatgctg agacctcaga tggtactttg ccagcaagac gaaacagacg 6121 ggccttcggg gacaagcaga gctgtctgca cccatttacg gaagatgatg ctgttgatcc 6181 caatgactct gacatagacc cagaatccag ggagaccgac ctgctcagtg ggatggacgg 6241 caaaggctcc taccagctga agggctggaa actcacccag caacagtttg tggccctttt 6301 gtggaagagg ctgctgattg ccagacggag ccggaagggt ttctttgctc agattgtcct 6361 gccagctgtc tttgtttgca ttgccctggt cttcagcctg attgtgccac cctttggcaa 6421 gtaccccagc ctggaacttc agccctggat gtataatgag cagtatacat ttgtcagtaa 6481 tgatgctccc gaggacatgg gcacccagga actcctgaat gctctgacca aagatccagg 6541 ctttgggacc cgctgtatgg aaggaaaccc aatcccagat accccttgct tggctgggga 6601 ggaggactgg accatcagcc ccgtccccca gagcatcgtg gacctcttcc agaatggaaa 6661 ctggaccatg aagaacccct cacctgcgtg ccagtgtagc agtgacaaaa tcaagaagat 6721 gctgcctgtg tgtcccccag gggcaggggg gctgccacct cctcagagaa aacagaaaac 6781 cgcagacatc cttcagaatc tgacaggaag aaatatttca gattacctgg tgaagacgta 6841 cgtgcagatc atagcaaaga gcttaaagaa taagatctgg gtgaacgagt ttcggtatgg 6901 cgggttttcc ctgggtgtca gtaattctca agcacttcct ccgagccatg aagttaatga 6961 tgctatcaag caaatgaaga aactcctgaa gctgaccaag gacagctccg cagatcgctt 7021 cctcagcagc ctgggaaggt tcatggcagg gctggatacg aaaaacaatg tcaaggtgtg 7081 gttcaataac aagggctggc atgctatcag ttcgttcctg aatgtcatca acaatgccat 7141 tctccgggcc aacctgcaga agggagagaa cccgagccag tatggaatca ctgctttcaa 7201 ccaccccttg aacctcacta aacagcagct ctcagaggtg gctctgatga ccacctctgt 7261 cgacgtcctc gtgtctatct gtgtcatctt tgcgatgtcc tttgtccctg ccagctttgt 7321 tgtgttcctg atccaggagc gtgtgagcaa agccaagcat cttcagttca tcagcggcgt 7381 gaagcctgtc atctactggc tgtccaattt tgtctgggat atgtgcaatt atgtggtccc 7441 tgctacactg gtcattatca tcttcatctg cttccagcag aagtcctatg tgtcctctac 7501 caacctgccc gttctagccc ttctgctctt gctgtatggg tggtcgatca ctcctctcat 7561 gtatccagca tcctttgtgt tcaagatccc cagcaccgcc tatgtggttc tcaccagtgt 7621 gaacctcttc atcggcatca atggcagtgt ggccactttc gtactggagc tgtttacaaa 7681 caataagctc aatgacatca atgacatcct gaagtctgtg tttcttatct tcccacattt 7741 ttgcctgggg cgagggctca tcgacatggt gaagaaccag gccatggccg atgccctgga 7801 gaggtttggg gagaaccgct tcgtctctcc gctctcttgg gacttggtag gacggaacct 7861 ttttgccatg gccgtggaag gggtggtgtt cttcctcatt actgttctga tccagtacag 7921 atttttcatc aggcccagac ctgtaaaggc gaagcttcct cctttgaatg acgaggatga 7981 ggatgtgagg cgggagagac agaggattct ggacggtggc ggacagaatg acatcctaga 8041 gatcaaggaa ctgaccaaga tctataggag gaagcggaag cctgcagtcg acaggatctg 8101 catcggcatc cctcccggag agtgctttgg actcctggga gttaacggag ctgggaagtc 8161 aacaactttc aagatgctga ctggagacac ccctgtgacc agaggggatg cgttccttaa 8221 caaaaacagc atcttatcaa atatccatga agtacaccag aacatgggct actgccctca 8281 gtttgacgcc atcacagagc tgctgacggg aagagagcat gtggagttct ttgccctcct 8341 gaggggagtc ccagaaaagg aagttggcaa ggttggtgaa tgggcaattc gcaaactggg 8401 cctggtaaag tatggagaaa aatatgccag taactacagt ggcggcaaca aacgaaagct 8461 ctccacagcc atggctttga ttggcggacc tcctgtggtg tttctggatg aaccaaccac 8521 aggcatggac cctaaagccc ggagattctt gtggaattgt gccctaagca ttgtcaagga 8581 ggggagatct gtagtcctta catctcatag tatggaagaa tgtgaagctc tttgtacaag 8641 gatggccata atggtcaatg gaaggttcag gtgccttggc agtgtccaac atctgaaaaa 8701 caggtttgga gatggttata caatagttgt acgaatagca ggctccaacc ctgacctgaa 8761 gcctgtccag gagttctttg gacttgcgtt tccgggaagt gtcctaaaag agaaacatcg 8821 aaacatgctt cagtaccagc ttccatcctc cttgtcatct ctagccagga tattcagcat 8881 cctctcccag agcaaaaagc gactccacat agaagactac tctgtctctc agacaacact 8941 tgaccaagta tttgtgaact ttgccaagga ccaaagtgat gatgaccact taaaggacct 9001 gtcactgcac aaaaaccaga cagttgtgga tgtggccgtt ctcacatcct ttttgcagga 9061 tgagaaagtg aaagaaagtt atgtaggatc cgactacaaa gaccatgacg gtgattataa 9121 agatcatgac atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga 9181 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg 9241 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag 9301 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc 9361 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct 9421 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg 9481 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt 9541 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct 9601 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt 9661 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga 9721 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg 9781 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg 9841 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc 9901 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt 9961 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg 10021 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct 10081 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg 10141 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga 10201 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta 10261 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta 10321 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca 10381 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggatc 10441 tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat 10501 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 10561 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 10621 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 10681 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 10741 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 10801 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 10861 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 10921 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 10981 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 11041 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa 11101 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 11161 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 11221 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 11281 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 11341 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 11401 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 11461 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 11521 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 11581 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg 11641 ccttcgccct cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag 11701 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 11761 ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct 11821 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 11881 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 11941 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 12001 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 12061 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 12121 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 12181 atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct 12241 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 12301 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc 12361 ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 12421 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 12481 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 12541 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 12601 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 12661 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 12721 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 12781 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 12841 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 12901 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 12961 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 13021 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 13081 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 13141 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 13201 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 13261 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 13321 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 13381 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 13441 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 13501 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 13561 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 13621 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 13681 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 13741 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 13801 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 13861 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 13921 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 13981 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 14041 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 14101 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 14161 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 14221 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 14281 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 14341 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 14401 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 14461 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 14521 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 14581 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 14641 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 14701 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag 14761 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 14821 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg 14881 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta 14941 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag 15001 ctg //