LOCUS       V040432                15003 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040432
VERSION     V040432
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..9085
                     /label="Abca1(NM_013454)"
                     /note="Abca1(NM_013454)"
                     /gene="Abca1"
     CDS             9092..9157
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        9201..9412
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             9425..9693
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             9728..10435
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             10445..10498
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             10499..11098
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    11099..11686
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             11760..11993
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    12065..12186
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      12205..12340
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(12378..12394)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    12402..12418
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12426..12456)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12471..12492
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12780..13368)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13539..14399)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(14400..14504)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     14978..14994
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggcttg ttggcctcag ttaaggctgc tgctgtggaa
     2341 gaatctgaca tttcgaagga gacaaacatg tcagctgtta ctggaagtgg cctggcctct
     2401 ctttatcttc ctgatcctga tctctgtacg cctgagctac ccaccctacg aacaacatga
     2461 gtgccacttt ccgaataaag ccatgccgtc tgcaggaacc ctcccctggg tacaggggat
     2521 tatctgtaat gccaacaacc cctgcttccg ttatccaact cccggcgagg ctcccggtgt
     2581 tgttggaaac tttaacaagt ccatcgtgtc tcgcctgttc tcagacgctc agaggcttct
     2641 tctgtacagc caaagagata ccagcattaa ggacatgcac aaggtcctga gaatgttacg
     2701 gcagatcaag catcccaact caaatttgaa gctccaggat tttctggtgg acaatgaaac
     2761 attctctgga ttcctgcagc acaatttgtc ccttccaaga tctactgtgg acagcctgct
     2821 gcaggcgaat gttggtctcc agaaggtatt tttgcaaggc taccaattac atttggccag
     2881 tctgtgtaac ggatcaaaat tagaagaaat tattcagctt ggtgatgcgg aagtttctgc
     2941 cctctgtggt ctaccgagga agaagctcga tgcagccgag agagtactgc gctacaacat
     3001 ggacatcctg aagccagttg tgacaaaact aaattccaca tctcatctcc cgacccagca
     3061 tctggctgaa gccaccacag tgttgcttga cagcttgggg ggcctggccc aagagctgtt
     3121 cagcacaaag agctggagcg acatgcggca ggaggtgatg tttctgacca acgtgaacag
     3181 ctccagctcc tccacccaga tctaccaggc agtgtcccgc atcgtgtgtg gtcacccaga
     3241 gggtgggggc ctgaagatca agtccctcaa ctggtacgag gataacaact acaaagccct
     3301 ctttggaggg aataacaccg aggaagacgt ggacaccttc tatgacaatt ctacaactcc
     3361 ttattgcaat gatttgatga agaacttgga gtctagtcct ctttctcgaa ttatttggaa
     3421 ggcactcaag ccactgcttg ttggaaagat tctctataca cctgacacac cagctacaag
     3481 gcaggtcatg gctgaggtga acaagacctt tcaggagttg gctgtgttcc atgacctgga
     3541 gggcatgtgg gaagaactca gcccccaaat ttggaccttc atggagaaca gccaagagat
     3601 ggaccttgtc cggacgctgt tagacagcag aggcaatgac cagttttggg aacagaagtt
     3661 ggatggatta gattggactg cccaagacat catggcgttt ctggccaaga acccagaaga
     3721 tgttcagtcc ccaaatggct ctgtgtatac ctggagagaa gctttcaatg agaccaacca
     3781 ggcaatccag acgatatctc gattcatgga gtgtgtcaac ctgaacaagc tggaacccat
     3841 tccgacagaa gtcaggctca tcaacaagtc catggagctg ctggacgaga ggaagttctg
     3901 ggctggcatc gtgttcacag gcatcactcc agatagtgtg gagctgcccc atcatgtaaa
     3961 gtacaagatc cggatggaca ttgacaacgt ggagagaact aataagatca aggatgggta
     4021 ctgggaccct ggtcctcggg ctgacccttt tgaagatatg cgctatgtct ggggcggctt
     4081 cgcctacttg caggatgtgg tggaacaggc catcatcaga gtgctgacgg gatctgagaa
     4141 gaaaacgggt gtctacgtgc aacagatgcc ctacccctgt tatgttgatg acatttttct
     4201 gcgggtcatg agccggtcaa tgcccctctt catgactcta gcctggatct actctgtcgc
     4261 tgtgatcatc aagagcattg tgtatgagaa ggaggctcgg ctgaaggaga ccatgcggat
     4321 catgggtctg gacaatggca tcctctggtt tagctggttt gttagcagcc tcatccctct
     4381 gcttgtgagc gctggcctgc tggtggtcat cttgaagtta ggaaacctgc tgccctatag
     4441 tgaccccagc gtggtgttcg tcttcctgtc tgtgtttgcc atggtgacca tcctacagtg
     4501 cttcctcatt agcacgctct tctcccgtgc caacctggca gcagcctgtg ggggcatcat
     4561 ctacttcacg ctgtacctgc cctatgtgct gtgcgtagcc tggcaggact atgtgggctt
     4621 ctccatcaag atctttgcta gcctgctgtc tcctgtggct tttggattcg gctgtgagta
     4681 tttcgccctt ttcgaggagc aaggtatcgg ggtccaatgg gacaatctct ttgagagccc
     4741 ggtggaggag gacggcttca atctcaccac tgcagtgtcc atgatgctct ttgacacctt
     4801 tctctatggc gtgatgacat ggtacatcga agccgtcttt ccaggacagt atggaattcc
     4861 caggccctgg tattttcctt gtaccaagtc atactggttt ggtgaggaaa ttgatgagaa
     4921 gagccaccct ggttccagcc agaagggagt gtcagaaatc tgcatggaag aggaacccac
     4981 tcatctgagg ctgggggtgt ccattcagaa cctggtgaag gtttaccgag atggcatgaa
     5041 ggttgctgtg gatggcttgg cgctcaactt ttacgaaggc cagattacct ccttcctggg
     5101 ccacaatgga gcagggaaga ccaccaccat gtcaatactg actgggctgt ttcccccaac
     5161 ttctggcacg gcctacatcc tggggaagga cattcgctcg gagatgagct ccatccggca
     5221 gaacctggga gtctgtcccc agcataatgt gctgtttgac atgctgactg tcgaagagca
     5281 catctggttc tatgcgcgcc taaaggggct ctcagagaag cacgtgaaag cagagatgga
     5341 gcagatggcc ctggatgttg gcttaccccc gagcaagctg aaaagcaaaa cgagtcagct
     5401 ctcaggtggg atgcagagaa agctgtctgt ggccttggcc ttcgtgggtg gatccaaggt
     5461 tgtcattctg gacgagccca cagccggggt ggacccgtac tctcgcaggg gaatatggga
     5521 actcctgcta aaataccggc aaggccgcac cattattttg tctacacacc acatggacga
     5581 agctgacatc cttggggaca gaattgccat catttcccat gggaagctgt gttgtgtggg
     5641 ctcctccctg tttttgaaaa accagttggg aacgggttac tatctgaccc tggttaagaa
     5701 agatgtggaa tcgtccctca gttcctgcag aaacagtagc agcaccgtgt cttgtctgaa
     5761 aaaggaggac agtgtttctc agagcagttc tgatgctggc ctgggcagcg accatgaaag
     5821 tgacacgctg accatcgatg tctctgctat ctccaacctc atcaggaagc acgtgtctga
     5881 agcccggctg gtggaggaca ttgggcacga gctgacctat gtgctgccgt acgaagccgc
     5941 gaaggaggga gcctttgtgg aactcttcca tgagattgat gaccggctct cagacctggg
     6001 catctccagt tatggcatct cggagaccac cctggaagaa atattcctca aagtggctga
     6061 agagagcggg gtggatgctg agacctcaga tggtactttg ccagcaagac gaaacagacg
     6121 ggccttcggg gacaagcaga gctgtctgca cccatttacg gaagatgatg ctgttgatcc
     6181 caatgactct gacatagacc cagaatccag ggagaccgac ctgctcagtg ggatggacgg
     6241 caaaggctcc taccagctga agggctggaa actcacccag caacagtttg tggccctttt
     6301 gtggaagagg ctgctgattg ccagacggag ccggaagggt ttctttgctc agattgtcct
     6361 gccagctgtc tttgtttgca ttgccctggt cttcagcctg attgtgccac cctttggcaa
     6421 gtaccccagc ctggaacttc agccctggat gtataatgag cagtatacat ttgtcagtaa
     6481 tgatgctccc gaggacatgg gcacccagga actcctgaat gctctgacca aagatccagg
     6541 ctttgggacc cgctgtatgg aaggaaaccc aatcccagat accccttgct tggctgggga
     6601 ggaggactgg accatcagcc ccgtccccca gagcatcgtg gacctcttcc agaatggaaa
     6661 ctggaccatg aagaacccct cacctgcgtg ccagtgtagc agtgacaaaa tcaagaagat
     6721 gctgcctgtg tgtcccccag gggcaggggg gctgccacct cctcagagaa aacagaaaac
     6781 cgcagacatc cttcagaatc tgacaggaag aaatatttca gattacctgg tgaagacgta
     6841 cgtgcagatc atagcaaaga gcttaaagaa taagatctgg gtgaacgagt ttcggtatgg
     6901 cgggttttcc ctgggtgtca gtaattctca agcacttcct ccgagccatg aagttaatga
     6961 tgctatcaag caaatgaaga aactcctgaa gctgaccaag gacagctccg cagatcgctt
     7021 cctcagcagc ctgggaaggt tcatggcagg gctggatacg aaaaacaatg tcaaggtgtg
     7081 gttcaataac aagggctggc atgctatcag ttcgttcctg aatgtcatca acaatgccat
     7141 tctccgggcc aacctgcaga agggagagaa cccgagccag tatggaatca ctgctttcaa
     7201 ccaccccttg aacctcacta aacagcagct ctcagaggtg gctctgatga ccacctctgt
     7261 cgacgtcctc gtgtctatct gtgtcatctt tgcgatgtcc tttgtccctg ccagctttgt
     7321 tgtgttcctg atccaggagc gtgtgagcaa agccaagcat cttcagttca tcagcggcgt
     7381 gaagcctgtc atctactggc tgtccaattt tgtctgggat atgtgcaatt atgtggtccc
     7441 tgctacactg gtcattatca tcttcatctg cttccagcag aagtcctatg tgtcctctac
     7501 caacctgccc gttctagccc ttctgctctt gctgtatggg tggtcgatca ctcctctcat
     7561 gtatccagca tcctttgtgt tcaagatccc cagcaccgcc tatgtggttc tcaccagtgt
     7621 gaacctcttc atcggcatca atggcagtgt ggccactttc gtactggagc tgtttacaaa
     7681 caataagctc aatgacatca atgacatcct gaagtctgtg tttcttatct tcccacattt
     7741 ttgcctgggg cgagggctca tcgacatggt gaagaaccag gccatggccg atgccctgga
     7801 gaggtttggg gagaaccgct tcgtctctcc gctctcttgg gacttggtag gacggaacct
     7861 ttttgccatg gccgtggaag gggtggtgtt cttcctcatt actgttctga tccagtacag
     7921 atttttcatc aggcccagac ctgtaaaggc gaagcttcct cctttgaatg acgaggatga
     7981 ggatgtgagg cgggagagac agaggattct ggacggtggc ggacagaatg acatcctaga
     8041 gatcaaggaa ctgaccaaga tctataggag gaagcggaag cctgcagtcg acaggatctg
     8101 catcggcatc cctcccggag agtgctttgg actcctggga gttaacggag ctgggaagtc
     8161 aacaactttc aagatgctga ctggagacac ccctgtgacc agaggggatg cgttccttaa
     8221 caaaaacagc atcttatcaa atatccatga agtacaccag aacatgggct actgccctca
     8281 gtttgacgcc atcacagagc tgctgacggg aagagagcat gtggagttct ttgccctcct
     8341 gaggggagtc ccagaaaagg aagttggcaa ggttggtgaa tgggcaattc gcaaactggg
     8401 cctggtaaag tatggagaaa aatatgccag taactacagt ggcggcaaca aacgaaagct
     8461 ctccacagcc atggctttga ttggcggacc tcctgtggtg tttctggatg aaccaaccac
     8521 aggcatggac cctaaagccc ggagattctt gtggaattgt gccctaagca ttgtcaagga
     8581 ggggagatct gtagtcctta catctcatag tatggaagaa tgtgaagctc tttgtacaag
     8641 gatggccata atggtcaatg gaaggttcag gtgccttggc agtgtccaac atctgaaaaa
     8701 caggtttgga gatggttata caatagttgt acgaatagca ggctccaacc ctgacctgaa
     8761 gcctgtccag gagttctttg gacttgcgtt tccgggaagt gtcctaaaag agaaacatcg
     8821 aaacatgctt cagtaccagc ttccatcctc cttgtcatct ctagccagga tattcagcat
     8881 cctctcccag agcaaaaagc gactccacat agaagactac tctgtctctc agacaacact
     8941 tgaccaagta tttgtgaact ttgccaagga ccaaagtgat gatgaccact taaaggacct
     9001 gtcactgcac aaaaaccaga cagttgtgga tgtggccgtt ctcacatcct ttttgcagga
     9061 tgagaaagtg aaagaaagtt atgtaggatc cgactacaaa gaccatgacg gtgattataa
     9121 agatcatgac atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga
     9181 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     9241 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     9301 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     9361 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     9421 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     9481 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     9541 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     9601 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     9661 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga
     9721 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     9781 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     9841 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     9901 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     9961 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
    10021 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
    10081 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
    10141 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
    10201 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
    10261 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
    10321 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
    10381 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggatc
    10441 tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat
    10501 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
    10561 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
    10621 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
    10681 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
    10741 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
    10801 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
    10861 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
    10921 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
    10981 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
    11041 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa
    11101 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
    11161 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
    11221 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
    11281 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
    11341 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
    11401 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
    11461 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
    11521 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
    11581 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg
    11641 ccttcgccct cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag
    11701 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
    11761 ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct
    11821 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    11881 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    11941 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
    12001 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
    12061 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    12121 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    12181 atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct
    12241 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    12301 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc
    12361 ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    12421 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    12481 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    12541 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    12601 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    12661 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    12721 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    12781 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    12841 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    12901 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    12961 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    13021 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    13081 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    13141 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    13201 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
    13261 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    13321 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    13381 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    13441 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    13501 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    13561 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    13621 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    13681 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    13741 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    13801 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    13861 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    13921 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    13981 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    14041 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    14101 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    14161 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    14221 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    14281 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    14341 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    14401 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    14461 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    14521 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    14581 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    14641 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
    14701 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
    14761 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    14821 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
    14881 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
    14941 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
    15001 ctg
//
