LOCUS       V039710                15766 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039710
VERSION     V039710
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..9792
                     /label="ATRX(NM_000489)"
                     /note="ATRX(NM_000489)"
                     /gene="ATRX"
     CDS             9799..9864
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        9908..10119
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             10132..10400
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      10429..10438
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             10459..11121
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             11191..11244
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             11245..11844
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    11845..12433
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             12507..12740
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    12812..12933
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      12973..13108
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(13141..13157)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    13165..13181
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(13189..13219)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    13234..13255
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(13543..14131)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(14302..15162)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(15163..15267)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     15741..15757
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga ccgctgagcc catgagtgaa
     2341 agcaagttga atacattggt gcagaagctt catgacttcc ttgcacactc atcagaagaa
     2401 tctgaagaaa caagttctcc tccacgactt gcaatgaatc aaaacacaga taaaatcagt
     2461 ggttctggaa gtaactctga tatgatggaa aacagcaagg aagagggaac tagctcttca
     2521 gaaaaatcca agtcttcagg atcgtcacga tcaaagagga aaccttcaat tgtaacaaag
     2581 tatgtagaat cagatgatga aaaacctttg gatgatgaaa ctgtaaatga agatgcgtct
     2641 aatgaaaatt cagaaaatga tattactatg cagagcttgc caaaaggtac agtgattgta
     2701 cagccagagc cagtgctgaa tgaagacaaa gatgatttta aagggcctga atttagaagc
     2761 agaagtaaaa tgaaaactga aaatctcaaa aaacgcggag aagatgggct tcatgggatt
     2821 gtgagctgca ctgcttgtgg acaacaggtc aatcattttc aaaaagattc catttataga
     2881 cacccttcat tgcaagttct tatttgtaag aattgcttta agtattacat gagtgatgat
     2941 attagccgtg actcagatgg aatggatgaa caatgtaggt ggtgtgcgga aggtggaaac
     3001 ttgatttgtt gtgacttttg ccataatgct ttctgcaaga aatgcattct acgcaacctt
     3061 ggtcgaaagg agttgtccac aataatggat gaaaacaacc aatggtattg ctacatttgt
     3121 cacccagagc ctttgttgga cttggtcact gcatgtaaca gcgtatttga gaatttagaa
     3181 cagttgttgc agcaaaataa gaagaagata aaagttgaca gtgaaaagag taataaagta
     3241 tatgaacata catccagatt ttctccaaag aagactagtt caaattgtaa tggagaagaa
     3301 aagaaattag atgattcctg ttctggctct gtaacctact cttattccgc actaattgtg
     3361 cccaaagaga tgattaagaa ggcaaaaaaa ctgattgaga ccacagccaa catgaactcc
     3421 agttatgtta aatttttaaa gcaggcaaca gataattcag aaatcagttc tgctacaaaa
     3481 ttacgtcagc ttaaggcttt taagtctgtg ttggctgata ttaagaaggc tcatcttgca
     3541 ttggaagaag acttaaattc cgagtttcga gcgatggatg ctgtaaacaa agagaaaaat
     3601 accaaagagc ataaagtcat agatgctaag tttgaaacaa aagcacgaaa aggagaaaaa
     3661 ccttgtgctt tggaaaagaa ggatatttca aagtcagaag ctaaactttc aagaaaacag
     3721 gtagatagtg agcacatgca tcagaatgtt ccaacagagg aacaaagaac aaataaaagt
     3781 accggtggtg aacataagaa atctgataga aaagaagaac ctcaatatga acctgccaac
     3841 acttctgaag atttagacat ggatattgtg tctgttcctt cctcagttcc agaagacatt
     3901 tttgagaatc ttgagactgc tatggaagtt cagagttcag ttgatcatca aggggatggc
     3961 agcagtggaa ctgaacaaga agtggagagt tcatctgtaa aattaaatat ttcttcaaaa
     4021 gacaacagag gaggtattaa atcaaaaact acagctaaag taacaaaaga attatatgtt
     4081 aaactcactc ctgtttccct ttctaattcc ccaattaaag gtgctgattg tcaggaagtt
     4141 ccacaagata aagatggcta taaaagttgt ggtctgaacc ccaagttaga gaaatgtgga
     4201 cttggacagg aaaacagtga taatgagcat ttggttgaaa atgaagtttc attactttta
     4261 gaggaatctg atcttcgaag atccccacgt gtaaagacta cacccttgag gcgaccgaca
     4321 gaaactaacc ctgtaacatc taattcagat gaagaatgta atgaaacagt taaggagaaa
     4381 caaaaactat cagttccagt gagaaaaaag gataagcgta attcttctga cagtgctata
     4441 gataatccta agcctaataa attgccaaaa tctaagcaat cagagactgt ggatcaaaat
     4501 tcagattctg atgaaatgct agcaatcctc aaagaggtga gcaggatgag tcacagttct
     4561 tcttcagata ctgatattaa tgaaattcat acaaaccata agactttgta tgatttaaag
     4621 actcaggcgg ggaaagatga taaaggaaaa aggaaacgaa aaagttctac atctggctca
     4681 gattttgata ctaaaaaggg caaatcagct aagagctcta taatttctaa aaagaaacga
     4741 caaacccagt ctgagtcttc taattatgac tcagaattag aaaaagagat aaagagcatg
     4801 agtaaaattg gtgctgccag aaccaccaaa aaaagaattc caaatacaaa agattttgac
     4861 tcttctgaag atgagaaaca cagcaaaaaa ggaatggata atcaagggca caaaaatttg
     4921 aagacctcac aagaaggatc atctgatgat gctgaaagaa aacaagagag agagactttc
     4981 tcttcagcag aaggcacagt tgataaagac acgaccatca tggaattaag agatcgactt
     5041 cctaagaagc agcaagcaag tgcttccact gatggtgtcg ataagctttc tgggaaagag
     5101 gagagtttta cttctttgga agttagaaaa gttgctgaaa ctaaagaaaa gagcaagcat
     5161 ctcaaaacca aaacatgtaa aaaagtacag gatggcttat ctgatattgc agagaaattc
     5221 ctaaagaaag accagagcga tgaaacttct gaagatgata aaaagcagag caaaaaggga
     5281 actgaagaaa aaaagaaacc ttcagacttt aagaaaaaag taattaaaat ggaacaacag
     5341 tatgaatctt catctgatgg cactgaaaag ttacctgagc gagaagaaat ttgtcatttt
     5401 cctaagggca taaaacaaat taagaatgga acaactgatg gagaaaagaa aagtaaaaaa
     5461 ataagagata aaacttctaa aaagaaggat gaattatctg attatgctga gaagtcaaca
     5521 gggaaaggag atagttgtga ctcttcagag gataaaaaga gtaagaatgg agcatatggt
     5581 agagagaaga aaaggtgcaa gttgcttgga aagagttcaa ggaagagaca agattgttca
     5641 tcatctgata ctgagaaata ttccatgaaa gaagatggtt gtaactcttc tgataagaga
     5701 ctgaaaagaa tagaattgag ggaaagaaga aatttaagtt caaagagaaa tactaaggaa
     5761 atacaaagtg gctcatcatc atctgatgct gaggaaagtt ctgaagataa taaaaagaag
     5821 aagcaaagaa cttcatctaa aaagaaggca gtcattgtca aggagaaaaa gagaaactcc
     5881 ctaagaacaa gcactaaaag gaagcaagct gacattacat cctcatcttc ttctgatata
     5941 gaagatgatg atcagaattc tataggtgag ggaagcagcg atgaacagaa aattaagcct
     6001 gtgactgaaa atttagtgct gtcttcacat actggatttt gccaatcttc aggagatgaa
     6061 gccttatcta aatcagtgcc tgtcacagtg gatgatgatg atgacgacaa tgatcctgag
     6121 aatagaattg ccaagaagat gcttttagaa gaaattaaag ccaatctttc ctctgatgag
     6181 gatggatctt cagatgatga gccagaagaa gggaaaaaaa gaactggaaa acaaaatgaa
     6241 gaaaacccag gagatgagga agcaaaaaat caagtcaatt ctgaatcaga ttcagattct
     6301 gaagaatcta agaagccaag atacagacat aggcttttgc ggcacaaatt gactgtgagt
     6361 gacggagaat ctggagaaga aaaaaagaca aagcctaaag agcataaaga agtcaaaggc
     6421 agaaacagaa gaaaggtgag cagtgaagat tcagaagatt ctgattttca ggaatcagga
     6481 gttagtgaag aagttagtga atccgaagat gaacagcggc ccagaacaag gtctgcaaag
     6541 aaagcagagt tggaagaaaa tcagcggagc tataaacaga aaaagaaaag gcgacgtatt
     6601 aaggttcaag aagattcatc cagtgaaaac aagagtaatt ctgaggaaga agaggaggaa
     6661 aaagaagagg aggaggaaga ggaggaggag gaggaagagg aggaggaaga tgaaaatgat
     6721 gattccaagt ctcctggaaa aggcagaaag aaaattcgga agattcttaa agatgataaa
     6781 ctgagaacag aaacacaaaa tgctcttaag gaagaggaag agagacgaaa acgtattgct
     6841 gagagggagc gtgagcgaga aaaattgaga gaggtgatag aaattgaaga tgcttcaccc
     6901 accaagtgtc caataacaac caagttggtt ttagatgaag atgaagaaac caaagaacct
     6961 ttagtgcagg ttcatagaaa tatggttatc aaattgaaac cccatcaagt agatggtgtt
     7021 cagtttatgt gggattgctg ctgtgagtct gtgaaaaaaa caaagaaatc tccaggttca
     7081 ggatgcattc ttgcccactg tatgggcctt ggtaagactt tacaggtggt aagttttctt
     7141 catacagttc ttttgtgtga caaactggat ttcagcacgg cgttagtggt ttgtcctctt
     7201 aatactgctt tgaattggat gaatgaattt gagaagtggc aagagggatt aaaagatgat
     7261 gagaagcttg aggtttctga attagcaact gtgaaacgtc ctcaggagag aagctacatg
     7321 ctgcagaggt ggcaagaaga tggtggtgtt atgatcatag gctatgagat gtatagaaat
     7381 cttgctcaag gaaggaatgt gaagagtcgg aaacttaaag aaatatttaa caaagctttg
     7441 gttgatccag gccctgattt tgttgtttgt gatgaaggcc atattctaaa aaatgaagca
     7501 tctgctgttt ctaaagctat gaattctata cgatcaagga ggaggattat tttaacagga
     7561 acaccacttc aaaataacct aattgagtat cattgtatgg ttaattttat caaggaaaat
     7621 ttacttggat ccattaagga gttcaggaat agatttataa atccaattca aaatggtcag
     7681 tgtgcagatt ctaccatggt agatgtcaga gtgatgaaaa aacgtgctca cattctctat
     7741 gagatgttag ctggatgtgt tcagaggaaa gattatacag cattaacaaa attcttgcct
     7801 ccaaaacacg aatatgtgtt agctgtgaga atgacttcta ttcagtgcaa gctctatcag
     7861 tactacttag atcacttaac aggtgtgggc aataatagtg aaggtggaag aggaaaggca
     7921 ggtgcaaagc ttttccaaga ttttcagatg ttaagtagaa tatggactca tccttggtgt
     7981 ttgcagctag actacattag caaagaaaat aagggttatt ttgatgaaga cagtatggat
     8041 gaatttatag cctcagattc tgatgaaacc tccatgagtt taagctccga tgattataca
     8101 aaaaagaaga aaaaagggaa aaaggggaaa aaagatagta gctcaagtgg aagtggcagt
     8161 gacaatgatg ttgaagtgat taaggtctgg aattcaagat ctcggggagg tggtgaagga
     8221 aatgtggatg aaacaggaaa caatccttct gtttctttaa aactggaaga aagtaaagct
     8281 acttcttctt ctaatccaag cagcccagct ccagactggt acaaagattt tgttacagat
     8341 gctgatgctg aggttttaga gcattctggg aaaatggtac ttctctttga aattcttcga
     8401 atggcagagg aaattgggga taaagtcctt gttttcagcc agtccctcat atctctggac
     8461 ttgattgaag attttcttga attagctagt agggagaaga cagaagataa agataaaccc
     8521 cttatttata aaggtgaggg gaagtggctt cgaaacattg actattaccg tttagatggt
     8581 tccactactg cacagtcaag gaagaagtgg gctgaagaat ttaatgatga aactaatgtg
     8641 agaggacgat tatttatcat ttctactaaa gcaggatctc taggaattaa tctggtagct
     8701 gctaatcgag taattatatt cgacgcttct tggaatccat cttatgacat ccagagtata
     8761 ttcagagttt atcgctttgg acaaactaag cctgtttatg tatataggtt cttagctcag
     8821 ggaaccatgg aagataagat ttatgatcgg caagtaacta agcagtcact gtcttttcga
     8881 gttgttgatc agcagcaggt ggagcgtcat tttactatga atgagcttac tgaactttat
     8941 acttttgagc cagacttatt agatgaccct aattcagaaa agaagaagaa gagggatact
     9001 cccatgctgc caaaggatac catacttgca gagctccttc agatacataa agaacacatt
     9061 gtaggatacc atgaacatga ttctcttttg gaccacaaag aagaagaaga gttgactgaa
     9121 gaagaaagaa aagcagcttg ggctgagtat gaagcagaga agaagggact gaccatgcgt
     9181 ttcaacatac caactgggac caatttaccc cctgtcagtt tcaactctca aactccttat
     9241 attcctttca atttgggagc cctgtcagca atgagtaatc aacagctgga ggacctcatt
     9301 aatcaaggaa gagaaaaagt tgtagaagca acaaacagtg tgacagcagt gaggattcaa
     9361 cctcttgagg atataatttc agctgtatgg aaggagaaca tgaatctctc agaggcccaa
     9421 gtacaggcgt tagcattaag tagacaagcc agccaggagc ttgatgttaa acgaagagaa
     9481 gcaatctaca atgatgtatt gacaaaacaa cagatgttaa tcagctgtgt tcagcgaata
     9541 cttatgaaca gaaggctcca gcagcagtac aatcagcagc aacagcaaca aatgacttat
     9601 caacaagcaa cactgggtca cctcatgatg ccaaagcccc caaatttgat catgaatcct
     9661 tctaactacc agcagattga tatgagagga atgtatcagc cagtggctgg tggtatgcag
     9721 ccaccaccat tacagcgtgc accaccccca atgagaagca aaaatccagg accttcccaa
     9781 gggaaatcaa tgggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     9841 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     9901 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     9961 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
    10021 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
    10081 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
    10141 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
    10201 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
    10261 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
    10321 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
    10381 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag
    10441 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac
    10501 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc
    10561 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
    10621 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
    10681 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
    10741 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
    10801 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
    10861 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc
    10921 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac
    10981 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
    11041 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
    11101 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc
    11161 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt
    11221 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc
    11281 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
    11341 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
    11401 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
    11461 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
    11521 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
    11581 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
    11641 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
    11701 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
    11761 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
    11821 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg
    11881 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
    11941 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
    12001 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
    12061 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
    12121 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
    12181 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
    12241 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
    12301 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
    12361 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
    12421 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
    12481 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
    12541 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    12601 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    12661 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    12721 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
    12781 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
    12841 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    12901 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
    12961 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
    13021 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
    13081 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
    13141 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    13201 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    13261 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    13321 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    13381 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    13441 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    13501 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    13561 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    13621 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    13681 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    13741 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    13801 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    13861 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    13921 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    13981 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    14041 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    14101 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    14161 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    14221 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    14281 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    14341 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    14401 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    14461 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    14521 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    14581 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    14641 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    14701 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    14761 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    14821 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    14881 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    14941 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    15001 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    15061 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    15121 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    15181 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    15241 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    15301 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    15361 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    15421 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    15481 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    15541 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    15601 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    15661 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    15721 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
