LOCUS       V028555                10807 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028555
VERSION     V028555
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4833
                     /label="ATG9A(NM_001077198)"
                     /note="ATG9A(NM_001077198)"
                     /gene="ATG9A"
     CDS             4840..4905
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4949..5160
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5173..5441
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5470..5479
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5500..6162
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6232..6285
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6286..6885
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6886..7474
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7548..7781
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7853..7974
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8014..8149
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8182..8198)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8206..8222
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8230..8260)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8275..8296
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8584..9172)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9343..10203)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10204..10308)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10782..10798
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cgcagtttga cactgaatac
     2341 cagcgcctag aggcctccta tagtgattca cccccagggg aggaggacct gttggtgcac
     2401 gtcgccgagg ggagcaagtc accttggcac catattgaaa accttgacct cttcttctct
     2461 cgagtttata atctgcacca gaagaatggc ttcacatgta tgctcatcgg ggagatcttt
     2521 gagctcatgc agttcctctt tgtggttgcc ttcactacct tcctggtcag ctgcgtggac
     2581 tatgacatcc tatttgccaa caagatggtg aaccacagtc ttcaccctac tgaacccgtc
     2641 aaggtcactc tgccagacgc ctttttgcct gctcaagtct gtagtgccag gattcaggaa
     2701 aatggctccc ttatcaccat cctggtcatt gctggtgtct tctggatcca ccggcttatc
     2761 aagttcatct ataacatttg ctgctactgg gagatccact ccttctacct gcacgctctg
     2821 cgcatcccta tgtctgccct tccgtattgc acgtggcaag aagtgcaggc ccggatcgtg
     2881 cagacgcaga aggagcacca gatctgcatc cacaaacgtg agctgacaga actggacatc
     2941 taccaccgca tcctccgttt ccagaactac atggtggcac tggttaacaa atccctcctg
     3001 cctctgcgct tccgcctgcc tggcctcggg gaagctgtct tcttcacccg tggtctcaag
     3061 tacaactttg agctgatcct cttctgggga cctggctctc tgtttctcaa tgaatggagc
     3121 ctcaaggccg agtacaaacg tggggggcaa cggctagagc tggcccagcg cctcagcaac
     3181 cgcatcctgt ggattggcat cgctaacttc ctgctgtgcc ccctcatcct catatggcaa
     3241 atcctctatg ccttcttcag ctatgctgag gtgctgaagc gggagccggg ggccctggga
     3301 gcacgctgct ggtcactcta tggccgctgc tacctccgcc acttcaacga gctggagcac
     3361 gagctgcagt cccgcctcaa ccgtggctac aagcccgcct ccaagtacat gaattgcttc
     3421 ttgtcacctc ttttgacact gctggccaag aatggagcct tcttcgctgg ctccatcctg
     3481 gctgtgctta ttgccctcac catttatgac gaagatgtgt tggctgtgga acatgtgctg
     3541 accaccgtca cactcctggg ggtcaccgtg accgtgtgca ggtcctttat cccggaccag
     3601 cacatggtgt tctgccctga gcagctgctc cgcgtgatcc tcgctcacat ccactacatg
     3661 cctgaccact ggcagggtaa tgcccaccgc tcgcagaccc gggacgagtt tgcccagctc
     3721 ttccagtaca aggcagtgtt cattttggaa gagttgctga gccccattgt cacacccctc
     3781 atcctcatct tctgcctgcg cccacgggcc ctggagatta tagacttctt ccgaaacttc
     3841 accgtggagg tcgttggtgt gggagatacc tgctcctttg ctcagatgga tgttcgccag
     3901 catggtcatc cccagtggct atctgctggg cagacagagg cctcagtgta ccagcaagct
     3961 gaggatggaa agacagagtt gtcactcatg cactttgcca tcaccaaccc tggctggcag
     4021 ccaccacgtg agagcacagc cttcctaggc ttcctcaagg agcaggttca gcgggatgga
     4081 gcagctgcta gcctcgccca agggggtctg ctccctgaaa atgccctctt tacgtctatc
     4141 cagtccttac aatctgagtc tgagcccctg agccttatcg caaatgtggt agctggctca
     4201 tcctgccggg gccctccact gcccagagac ctgcagggct ccaggcacag ggctgaagtc
     4261 gcctctgccc tgcgctcctt ctccccgctg caacccgggc aggcgcccac aggccgggct
     4321 cacagcacca tgacaggctc tggggtggat gccaggacag ccagctccgg gagcagcgtg
     4381 tgggaaggac agctgcagag cctggtgctg tcagaatatg catccacaga gatgagcctg
     4441 catgccctct atatgcacca gctccacaag cagcaggccc aggctgaacc tgagcggcat
     4501 gtatggcacc gccgggagag tgatgagagt ggagaaagcg cccctgatga agggggagag
     4561 ggcgcccggg ccccccagtc tatccctcgc tctgctagct atccctgtgc agcaccccgg
     4621 cctggagctc ctgagaccac tgccctgcat gggggcttcc agaggcgcta cggtggcatc
     4681 acagatcctg gcacagtgcc cagggttccc tctcatttct ctcggctgcc tcttggaggg
     4741 tgggcagaag atgggcagtc ggcatcaagg caccctgagc ccgtgcccga agagggctcg
     4801 gaggatgagc taccccctca ggtgcacaag gtaggatccg actacaaaga ccatgacggt
     4861 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     4921 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     4981 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     5041 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     5101 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5161 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     5221 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5281 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     5341 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     5401 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     5461 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     5521 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     5581 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     5641 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     5701 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     5761 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     5821 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     5881 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     5941 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     6001 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     6061 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     6121 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     6181 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     6241 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     6301 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6361 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6421 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6481 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6541 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6601 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6661 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6721 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6781 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6841 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     6901 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6961 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7021 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7081 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7141 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7201 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7261 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7321 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7381 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7441 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     7501 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7561 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     7621 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7681 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7741 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7801 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     7861 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7921 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7981 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8041 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8101 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     8161 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8221 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8281 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8341 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8401 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8461 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8521 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8581 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8641 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8701 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8761 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8821 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8881 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8941 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9001 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     9061 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9121 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9181 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9241 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9301 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9361 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9421 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9481 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9541 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9601 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9661 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9721 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9781 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9841 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9901 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9961 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10021 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10081 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10141 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10201 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10261 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10321 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    10381 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    10441 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    10501 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    10561 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    10621 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    10681 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    10741 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    10801 caagctg
//
