LOCUS       V038080                10217 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038080
VERSION     V038080
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4218
                     /label="ATAD3A(NM_018188)"
                     /note="ATAD3A(NM_018188)"
                     /gene="ATAD3A"
     CDS             4225..4251
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4255..4281
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4285..4311
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4376..4587
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4600..4868
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4897..4906
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4927..5589
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5659..5712
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5713..6312
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6313..6900
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6974..7207
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7279..7400
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7419..7554
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7592..7608)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7616..7632
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7640..7670)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7685..7706
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7994..8582)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8753..9613)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9614..9718)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10192..10208
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt cgtggctctt cggcattaac
     2341 aagggcccca agggtgaagg cgcggggccg ccgccgcctt tgccgcccgc gcagcccggg
     2401 gccgagggcg gcggggaccg cgggttggga gaccggccgg cgcccaagga caaatggagc
     2461 aacttcgacc ccaccggcct ggagcgcgcc gccaaggcgg cgcgcgagct ggagcactcg
     2521 cgttatgcca aggacgccct gaatctggca cagatgcagg agcagacgct gcagttggag
     2581 caacagtcca agctcaaaat gcggctggaa gccctgagcc tgctgcacac actagtctgg
     2641 gcatggagtc tctgccgtgc cggagccgtg cagacacagg agcggctgtc aggcagtgcc
     2701 agccctgagc aagtgccagc tggtgagtgc tgtgctctgc aggagtatga ggccgccgtg
     2761 gagcagctca agagcgagca gatccgggcg caggctgagg agaggaggaa gaccctgagc
     2821 gaggagaccc ggcagcacca ggccagggcc cagtatcaag acaagctggc ccggcagcgc
     2881 tacgaggacc aactgaagca gcagcaactt ctcaatgagg agaatttacg gaagcaggag
     2941 gagtccgtgc agaagcagga agccatgcgg cgagccaccg tggagcggga gatggagctg
     3001 cggcacaaga atgagatgct gcgagtggag gccgaggccc gggcgcgcgc caaggccgag
     3061 cgggagaatg cagacatcat ccgcgagcag atccgcctga aggcggccga gcaccgtcag
     3121 accgtcttgg agtccatcag gacggctggc accttgtttg gggaaggatt ccgtgccttt
     3181 gtgacagact gggacaaagt gacagccacg gtggctgggc tgacgctgct ggctgttggg
     3241 gtctactcag ccaagaatgc cacgcttgtc gccggccgct tcatcgaggc tcggctgggg
     3301 aagccgtccc tagtgaggga gacgtcccgc atcacggtgc ttgaggcgct gcggcacccc
     3361 atccaggtca gccggcggct cctcagtcga ccccaggacg cgctggaggg tgttgtgctc
     3421 agtcccagcc tggaagcacg ggtgcgcgac atcgccatag caacaaggaa caccaagaag
     3481 aaccgcagcc tgtacaggaa catcctgatg tacgggccac caggcaccgg gaagacgctg
     3541 tttgccaaga aactcgccct gcactcaggc atggactacg ccatcatgac aggcggggac
     3601 gtggccccca tggggcggga aggcgtgacc gccatgcaca agctctttga ctgggccaat
     3661 accagccggc gcggcctcct gctctttgtg gatgaagcgg acgccttcct tcggaagcga
     3721 gccaccgaga agataagcga ggacctcagg gccacactga acgccttcct gtaccgcacg
     3781 ggccagcaca gcaacaagtt catgctggtc ctggccagca accaaccaga gcagttcgac
     3841 tgggccatca atgaccgcat caatgagatg gtccacttcg acctgccagg gcaggaggaa
     3901 cgggagcgcc tggtgagaat gtattttgac aagtatgttc ttaagccggc cacagaagga
     3961 aagcagcgcc tgaagctggc ccagtttgac tacgggagga agtgctcgga ggtcgctcgg
     4021 ctgacggagg gcatgtcggg ccgggagatc gctcagctgg ccgtgtcctg gcaggccacg
     4081 gcgtatgcct ccgaggacgg ggtcctgacc gaggccatga tggacacccg cgtgcaagat
     4141 gctgtccagc agcaccagca gaagatgtgc tggctgaagg cggaagggcc tgggcgtggg
     4201 gacgagccct ccccatccct cgagtaccca tacgacgtac ctgattatgc aacttatcct
     4261 tatgatgtac ctgattatgc tagctatcct tatgatgtcc cagactacgc atagggatcc
     4321 ctgcgatatc cagcgcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     4381 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     4441 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     4501 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     4561 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     4621 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     4681 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     4741 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     4801 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     4861 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc
     4921 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
     4981 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag
     5041 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
     5101 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
     5161 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
     5221 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc
     5281 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
     5341 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc
     5401 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc
     5461 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
     5521 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc
     5581 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc
     5641 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag
     5701 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5761 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     5821 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     5881 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     5941 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6001 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6061 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6121 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6181 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6241 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6301 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6361 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6421 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6481 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6541 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6601 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6661 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     6721 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6781 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6841 cttccgcgtc tccgccttcg ccctcagacg agtcggatct ccctttggcc gcctccccgc
     6901 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6961 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct
     7021 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7081 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7141 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7201 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7261 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7321 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7381 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     7441 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7501 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact
     7561 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7621 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7681 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7741 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7801 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7861 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7921 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7981 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8041 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8101 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8161 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8221 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8281 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8341 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8401 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8461 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8521 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8581 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8641 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8701 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8761 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8821 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8881 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8941 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9001 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9061 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9121 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9181 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9241 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9301 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9361 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9421 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9481 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9541 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9601 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9661 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9721 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     9781 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     9841 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     9901 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     9961 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    10021 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
    10081 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    10141 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    10201 cggccagtgc caagctg
//
