LOCUS       V036759                12073 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036759
VERSION     V036759
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6099
                     /label="ARHGAP29(NM_004815)"
                     /note="ARHGAP29(NM_004815)"
                     /gene="ARHGAP29"
     CDS             6106..6171
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6215..6426
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6439..6707
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6736..6745
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6766..7428
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7498..7551
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7552..8151
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8152..8740
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8814..9047
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9119..9240
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9280..9415
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9448..9464)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9472..9488
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9496..9526)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9541..9562
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9850..10438)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10609..11469)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11470..11574)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12048..12064
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga ttgctcacaa acagaaaaag
     2341 acaaagaaaa aacgtgcttg ggcatcaggt caactctcta ctgatattac aacttctgaa
     2401 atggggctca agtccttaag ttccaactct atttttgatc cggattacat caaggagttg
     2461 gtgaatgata tcaggaagtt ctcccacatg ttactatatt tgaaagaagc catattttca
     2521 gactgtttta aagaagttat tcatatacgt ctagaggaac tgctccgtgt tttaaagtct
     2581 ataatgaata aacatcagaa cctcaattct gttgatcttc aaaatgctgc agaaatgctc
     2641 actgcaaaag tgaaagctgt gaacttcaca gaagttaatg aagaaaacaa aaacgatctc
     2701 ttccaggaag tgttttcttc tattgaaact ttggcattta cctttggaaa tatccttaca
     2761 aacttcctta tgggagatgt aggcaatgat tcattattgc gactgcctgt ttctcgagaa
     2821 actaagtcgt ttgaaaatgt ttctgtggaa tcagtggact catccagtga aaaaggaaat
     2881 ttttcccctt tagaactaga caacgtgctg ttaaagaaca ctgactctat cgagctggct
     2941 ttgtcatatg ctaaaacttg gtcaaaatat actaagaaca tagtttcatg ggttgaaaaa
     3001 aagcttaact tggaattgga gtccactaga aatatggtca agttggcaga ggcaactaga
     3061 actaacattg gaattcagga gttcatgcca ctgcagtctc tgtttactaa tgctcttctt
     3121 aatgatatag aaagcagtca ccttttacaa caaacaattg cagctctcca ggctaacaaa
     3181 tttgtgcagc ctctacttgg aaggaaaaat gaaatggaaa aacaaaggaa agaaataaaa
     3241 gagctttgga aacaggagca aaataaaatg cttgaagcag agaatgctct caaaaaggca
     3301 aaattattat gcatgcaacg tcaagatgaa tatgagaaag caaagtcttc catgtttcgt
     3361 gcagaagagg agcatctgtc ttcaagtggc ggattagcaa aaaatctcaa caagcaacta
     3421 gaaaaaaagc gaaggttgga agaggaggct ctccaaaaag tagaagaagc aaatgaactt
     3481 tacaaagttt gtgtgacaaa tgttgaagaa agaagaaatg atctagaaaa taccaaaaga
     3541 gaaattttag cacaactccg gacacttgtt ttccagtgtg atcttaccct taaagctgta
     3601 acagttaacc tcttccacat gcagcatctg caggctgctt cccttgcaga cagtttacag
     3661 tctctctgtg atagtgccaa actctatgac ccaggccaag agtacagtga atttgtcaag
     3721 gccacaaatt caactgaaga agaaaaagtt gatggaaatg taaataaaca tttaaatagt
     3781 tcccaacctt caggatttgg acctgccaac tctttagagg atgttgtacg ccttcctgac
     3841 agttctaata aaattgaaga ggacagatgc tctaacagtg cagatataac aggtccttcc
     3901 tttataagat catggacatt tgggatgttt agtgattctg agagcactgg agggagcagc
     3961 gaatctagat ctctggattc agaatctata agtccaggag actttcatcg aaaacttcca
     4021 cgaacaccat ccagtggaac tatgtcctct gcagatgatc tagatgaaag agagccacct
     4081 tccccttcag aaactggacc caattccctt ggaacattta agaaaacatt gatgtcaaag
     4141 gcagctctca cacacaagtt tcgcaaattg agatccccca cgaaatgtag ggattgtgaa
     4201 ggcattgtag tgttccaagg tgttgaatgt gaagagtgtc tccttgtttg tcatcgaaag
     4261 tgtttggaaa atttagtcat tatttgtggt catcagaaac ttccaggaaa aatacactta
     4321 tttggagcag aattcacaca agttgcaaaa aaggaaccag atggtatccc ttttatactc
     4381 aaaatatgtg cctcagagat tgaaaataga gctttgtgtc tacagggaat ttatcgtgtg
     4441 tgtggaaaca aaataaaaac tgaaaaattg tgtcaagctt tggaaaatgg aatgcacttg
     4501 gtagatattt cagaatttag ttcacatgat atctgtgacg tcttgaaatt ataccttcgg
     4561 cagctcccag aaccatttat tttatttcga ttgtacaagg aatttataga ccttgcaaaa
     4621 gagatccaac atgtaaatga agaacaagag acaaaaaaga atagtcttga agacaaaaaa
     4681 tggccaaata tgtgtataga aataaaccga attcttctaa aaagcaaaga ccttctaaga
     4741 caattgccag catcaaattt taacagtctt catttcctta tagtacatct aaagcgggta
     4801 gtagatcatg cagaagaaaa caagatgaac tccaaaaact tgggggtgat atttggacca
     4861 agtctcatta ggccaaggcc cacaactgct cctatcacca tctcctccct tgcagagtat
     4921 tcaaatcaag cacgcttggt agagtttctc attacttact cacagaagat cttcgatggg
     4981 tccctacaac cacaagatgt tatgtgtagc ataggtgttg ttgatcaagg ctgttttcca
     5041 aagcctctgt tatcaccaga agaaagagac attgaacgtt ccatgaagtc actatttttt
     5101 tcttcaaagg aagatatcca tacttcagag agtgaaagca aaatttttga acgagctaca
     5161 tcatttgagg aatcagaacg caagcaaaat gcgttaggaa aatgtgatgc atgtctcagt
     5221 gacaaagcac agttgcttct agaccaagag gctgaatcag catcccaaaa gatagaagat
     5281 ggtaaaaccc ctaagccact ttctctgaaa tctgataggt caacaaacaa tgtggagagg
     5341 catactccaa ggaccaagat tagacctgta agtttgcctg tagatagact acttcttgca
     5401 agtcctccta atgagagaaa tggcagaaat atgggaaatg taaatttaga caagttttgc
     5461 aagaatcctg cctttgaagg agttaataga aaagacgctg ctactactgt ttgttccaaa
     5521 tttaatggct ttgaccagca aactctacag aaaattcagg acaaacagta tgaacaaaac
     5581 agcctaactg ccaagactac aatgatcatg cccagtgcac tccaggaaaa aggagtgaca
     5641 acaagcctcc agattagtgg ggaccattct atcaatgcca ctcaacccag taagccatat
     5701 gcagagccag tcaggtcagt gagagaggca tctgagagac ggtcttcaga ttcctaccct
     5761 ctcgctcctg tcagagcacc cagaacactg cagcctcaac attggacaac attttataaa
     5821 ccacatgctc ccatcatcag tatcaggggg aatgaggaga agccagcttc accctcagca
     5881 gcagtgcctc ctggcacaga tcacgatccc cacggtctcg tggtgaagtc aatgccagac
     5941 ccagacaaag catcagcttg tcctgggcaa gcaactggtc aacctaaaga agactctgag
     6001 gagcttggct tgcctgatgt gaatccaatg tgtcagagac caaggctaaa acgaatgcaa
     6061 cagtttgaag acctcgaagg tgaaattcca caatttgtgg gatccgacta caaagaccat
     6121 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gtagcggccg
     6181 cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     6241 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     6301 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     6361 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     6421 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     6481 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     6541 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     6601 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     6661 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     6721 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc
     6781 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag
     6841 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc
     6901 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac
     6961 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc
     7021 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag
     7081 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg
     7141 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc
     7201 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac
     7261 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag
     7321 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg
     7381 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct
     7441 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag
     7501 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag
     7561 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     7621 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     7681 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     7741 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     7801 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     7861 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     7921 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     7981 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     8041 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     8101 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct
     8161 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     8221 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     8281 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     8341 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     8401 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     8461 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     8521 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     8581 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     8641 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     8701 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt taagaccaat
     8761 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     8821 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt
     8881 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8941 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     9001 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     9061 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     9121 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9181 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9241 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     9301 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9361 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac
     9421 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     9481 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9541 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9601 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9661 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9721 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9781 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10261 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    10321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10441 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10501 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10561 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10621 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10681 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10741 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10801 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10861 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10921 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10981 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11041 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11101 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11161 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11221 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11281 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11341 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11401 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11461 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11521 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11581 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    11641 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    11701 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    11761 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    11821 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    11881 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    11941 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    12001 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    12061 cagtgccaag ctg
//
