LOCUS       V039886                11207 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039886
VERSION     V039886
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2311..2320
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             3697..3720
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             3748..5301
                     /label="APEX2(NM_014481)"
                     /note="APEX2(NM_014481)"
                     /gene="APEX2"
     promoter        5366..5577
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5590..5858
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5887..5896
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5917..6579
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6649..6702
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6703..7302
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7303..7890
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7964..8197
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8269..8390
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8409..8544
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8582..8598)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8606..8622
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8630..8660)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8675..8696
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8984..9572)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9743..10603)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10604..10708)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11182..11198
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agtcctcacc catcccccag
     2341 tcatcaggga actcttccac tttggggagg gtccctcaaa ccccaggtcc ctctactgcc
     2401 agtggggtcc cggaggtggg gctacgggat gttgcttcgg aatctgtggc cctcttcttc
     2461 atgctcctgc tggacttgac tgctgtggct ggcaatgccg ctgtgatggc cgtgatcgcc
     2521 aagacgcctg ccctccgaaa atttgtcttc gtcttccacc tctgcctggt ggacctgctg
     2581 gctgccctga ccctcatgcc cctggccatg ctctccagct ctgccctctt tgaccacgcc
     2641 ctctttgggg aggtggcctg ccgcctctac ttgtttctga gcgtgtgctt tgtcagcctg
     2701 gccatcctct cggtgtcagc catcaatgtg gagcgctact attacgtagt ccaccccatg
     2761 cgctacgagg tgcgcatgac gctggggctg gtggcctctg tgctggtggg tgtgtgggtg
     2821 aaggccttgg ccatggcttc tgtgccagtg ttgggaaggg tctcctggga ggaaggagct
     2881 cccagtgtcc ccccaggctg ttcactccag tggagccaca gtgcctactg ccagcttttt
     2941 gtggtggtct ttgctgtcct ttactttctg ttgcccctgc tcctcatact tgtggtctac
     3001 tgcagcatgt tccgagtggc ccgcgtggct gccatgcagc acgggccgct gcccacgtgg
     3061 atggagacac cccggcaacg ctccgaatct ctcagcagcc gctccacgat ggtcaccagc
     3121 tcgggggccc cccagaccac cccacaccgg acgtttgggg gagggaaagc agcagtggtt
     3181 ctcctggctg tggggggaca gttcctgctc tgttggttgc cctacttctc tttccacctc
     3241 tatgttgccc tgagtgctca gcccatttca actgggcagg tggagagtgt ggtcacctgg
     3301 attggctact tttgcttcac ttccaaccct ttcttctatg gatgtctcaa ccggcagatc
     3361 cggggggagc tcagcaagca gtttgtctgc ttcttcaagc cagctccaga ggaggagctg
     3421 aggctgccta gccgggaggg ctccattgag gagaacttcc tgcagttcct tcaggggact
     3481 ggctgtcctt ctgagtcctg ggtttcccga cccctaccca gccccaagca ggagccacct
     3541 gctgttgact ttcgaatccc aggccagata gctgaggaga cctctgagtt cctggagcag
     3601 caactcacca gcgacatcat catgtcagac agctacctcc gtcctgccgc ctcaccccgg
     3661 ctggagtcag gatccggtgg aggcggatca ggtggagact acaaggacga cgatgacaag
     3721 ggtagtggtg gaggcggatc aggcggtatg ttgcgcgtgg tgagctggaa catcaatggg
     3781 attcggagac ccctgcaagg ggtggcaaat caggaaccca gcaactgtgc cgccgtggcc
     3841 gtggggcgca ttttggacga gctggatgcg gatatcgtct gtctccagga aaccaaagtg
     3901 accagggatg cactgacaga gcccctggct atcgttgagg gttataactc ctatttcagc
     3961 ttcagccgca accgtagcgg ctattctggt gtagccacct tctgtaagga caatgctacc
     4021 ccagtggctg ctgaagaagg cctgagtggc ctgtttgcca cccagaatgg ggatgttggt
     4081 tgctatggaa acatggatga gtttacccaa gaggaactcc gggctctgga tagtgagggc
     4141 agggccctcc tcacacagca taagatccgc acatgggaag gtaaggagaa gaccttgacc
     4201 ctaatcaacg tgtactgccc ccatgcggac cctgggaggc ctgagcggct agtctttaag
     4261 atgcgcttct atcgtttgct gcaaatccga gcagaagccc tcctggcggc aggcagccat
     4321 gtgatcattc tgggtgacct gaatacagcc caccgcccca ttgaccactg ggatgcagtc
     4381 aacctggaat gctttgaaga ggacccaggg cgcaagtgga tggacagctt gctcagtaac
     4441 ttggggtgcc agtctgcctc tcatgtaggg cccttcatcg atagctaccg ctgcttccaa
     4501 ccaaagcagg agggggcctt cacctgctgg tcagcagtca ctggcgcccg ccatctcaac
     4561 tatggctccc ggcttgacta tgtgctgggg gacaggaccc tggtcataga cacctttcag
     4621 gcctctttcc tgctgcctga ggtgatgggc tctgaccact gccctgtggg tgcagtcttg
     4681 agtgtgtcct ctgtgcctgc aaaacagtgc ccacctctgt gcacccgctt cctccctgag
     4741 tttgcaggca cccagctcaa gatccttcgc ttcctagttc ctctcgaaca aagtcctgtg
     4801 ttggagcagt cgacgctgca gcacaacaat caaacccggg tacagacatg ccaaaacaaa
     4861 gcccaagtgc gctcaaccag gcctcagccc agtcaggttg gctctagcag aggccagaaa
     4921 aacctgaaga gctactttca gccctcccct agctgtcccc aagcctctcc tgacatagag
     4981 ctgcctagcc taccactgat gagcgccctc atgaccccga agactccaga agagaaggca
     5041 gtggccaaag tggtgaaggg gcaggccaag acttcagaag ccaaagatga gaaggagtta
     5101 cggacctcat tctggaagtc tgtgctggcg gggcccttgc gcacacccct ctgtgggggc
     5161 cacagggagc catgtgtgat gcgtactgtg aagaagccag gacccaactt gggccgccgc
     5221 ttctacatgt gtgccaggcc ccggggtcct cccactgacc cctcctcccg gtgcaacttc
     5281 ttcctctgga gcaggcccag ctgaggatcc ctgcgatatc cagcgcggcc gcgaaggatc
     5341 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     5401 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     5461 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     5521 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     5581 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     5641 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     5701 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     5761 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     5821 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     5881 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     5941 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     6001 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     6061 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     6121 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     6181 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     6241 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     6301 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     6361 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     6421 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     6481 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
     6541 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
     6601 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
     6661 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     6721 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     6781 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     6841 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     6901 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6961 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     7021 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     7081 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     7141 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     7201 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     7261 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     7321 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     7381 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     7441 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     7501 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     7561 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     7621 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     7681 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     7741 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7801 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg
     7861 agtcggatct ccctttggcc gcctccccgc ctggtacctt taagaccaat gacttacaag
     7921 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7981 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     8041 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8101 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8161 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     8221 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     8281 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8341 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8401 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8461 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8521 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     8581 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8641 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8701 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8761 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8821 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8881 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8941 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9001 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9061 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9121 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9181 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9241 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9301 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9361 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9421 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9481 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9541 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9601 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9661 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9721 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9781 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9841 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9901 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9961 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10021 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10081 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10141 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10201 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10261 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10321 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10381 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10441 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10501 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10561 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10621 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10681 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
    10741 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    10801 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
    10861 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    10921 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    10981 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
    11041 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    11101 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    11161 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
