LOCUS       V038542                11269 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038542
VERSION     V038542
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5295
                     /label="ANKRD44(NM_001195144)"
                     /note="ANKRD44(NM_001195144)"
                     /gene="ANKRD44"
     CDS             5302..5367
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5411..5622
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5635..5903
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5932..5941
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5962..6624
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6694..6747
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6748..7347
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7348..7936
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8010..8243
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8315..8436
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8476..8611
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8644..8660)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8668..8684
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8692..8722)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8737..8758
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9046..9634)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9805..10665)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10666..10770)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11244..11260
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cagtgctcaa actcaccgac
     2341 cagccaccat tggttcaggc aatcttcagc ggtgatccag aggagatccg gatgctcatc
     2401 cataaaactg aagatgtgaa tactctggat tctgagaaac gaacccctct tcatgtggcc
     2461 gcatttctgg gagatgcaga gatcattgaa ctcctgattt tgtcaggagc tcgtgtaaat
     2521 gccaaggaca acatgtggct gactccactg caccgggctg ttgcttccag aagtgaagaa
     2581 gcagtacagg ttttgattaa gcactcagct gatgtcaatg caagggacaa gaactggcag
     2641 acccctcttc atgtggcagc agccaacaag gctgtcaaat gtgcagaagt gatcattccc
     2701 ctgctgagca gtgtcaatgt ctccgaccga ggggggcgca cagccttgca ccatgcggct
     2761 ctgaacggcc acgtggagat ggtcaattta ctcttggcca aaggggcaaa tatcaatgca
     2821 tttgacaaga aggaccggcg tgctctgcac tgggcagcat acatgggcca cttggatgtt
     2881 gtagcattgc tcattaacca tggcgcagaa gtgacctgta aggataagaa gggttatacc
     2941 cctctgcatg ctgcagcctc caatggacag attaatgttg tcaagcatct cctgaacctg
     3001 ggggtggaga ttgatgaaat caatgtctat ggaaatacag cgcttcacat cgcctgctac
     3061 aatggacagg atgctgtggt taacgagttg attgactacg gtgctaacgt gaaccagcca
     3121 aacaataatg ggttcacccc tttgcatttt gctgctgcct ccactcatgg tgctttgtgt
     3181 cttgaattgt tagtaaacaa cggggcagat gttaacattc agagtaaaga tggcaaaagt
     3241 ccactgcaca tgacagctgt ccatggaagg ttcacacggt cacagaccct cattcagaat
     3301 ggaggtgaaa ttgactgtgt ggataaggac ggcaacactc ctctccatgt ggctgcaaga
     3361 tacggtcatg agcttttgat taacacctta ataaccagcg gagctgacac agccaagtgt
     3421 ggaatccata gcatgttccc tttacattta gctgccctaa atgctcactc tgactgctgc
     3481 agaaagttgt tatcatcggg ctttgaaata gacaccccag ataaatttgg aagaacgtgc
     3541 cttcatgctg ctgctgcagg aggtaatgtg gaatgtataa aactcttgca gagcagcgga
     3601 gcagatttcc ataaaaagga caagtgtggg aggacccctt tgcactatgc agctgcgaat
     3661 tgtcatttcc actgtattga gacattagtg accacagggg ccaacgttaa tgaaacagat
     3721 gactggggac gcacagcttt gcattacgcc gctgcatcag acatggatag aaataagact
     3781 atcttaggaa atgcccatga taattcagaa gaacttgaaa gagccaggga gctgaaggaa
     3841 aaggaagcca cactatgtct agagtttctg cttcaaaatg atgcaaatcc atctatccgg
     3901 gacaaggaag gttacaatag catacattat gctgccgcct atgggcacag gcagtgtctg
     3961 gaattgcttt tggaaagaac aaacagtgga tttgaagaat cagattctgg tgctactaag
     4021 agtccactcc acttagctgc ctacaatggg caccatcaag ccttggaagt ccttctgcag
     4081 tcgttggtgg acctggacat cagggatgag aaaggccgca ctgctctgga tctggctgcc
     4141 tttaaaggac acacagaatg tgtggaagcg cttatcaatc agggcgcatc catctttgtg
     4201 aaagacaatg taaccaaaag aaccccactt catgcctcag taattaatgg tcacacactg
     4261 tgtttacggc tgttgctaga aattgcagac aacccggagg cggtcgatgt gaaagatgcc
     4321 aaaggacaaa caccactgat gcttgcagta gcatatggac atattgacgc tgtttcattg
     4381 ttacttgaaa aggaagccaa cgtagacact gttgacatcc taggatgcac agctttacac
     4441 agagggatta tgacaggaca cgaggaatgt gtgcaaatgc tgctggaaca agaagtgtca
     4501 attctctgta aagattccag agggaggacg cccttgcact atgcagctgc tcgtggccac
     4561 gccacgtggc tgagcgagct gctccaaatg gctctttctg aggaggactg ttgtttcaaa
     4621 gataaccaag gctacacgcc gctgcactgg gcttgttaca atggtaatga aaactgtata
     4681 gaggtacttt tggagcaaaa atgttttcgc aaatttatcg gtaatccctt tactccactg
     4741 cactgtgcaa taatcaatga tcatgggaat tgtgcatcat tgctgcttgg ggccatagat
     4801 tccagtatcg tcagttgtag agatgacaaa ggcaggacac cccttcatgc ggcagcattt
     4861 gctgatcatg tggagtgctt gcagcttctt ctgagacaca gtgctccagt gaacgcagta
     4921 gataattcag ggaaaacagc actgatgatg gctgctgaga atgggcaggc aggcgctgtg
     4981 gatattttgg tgaacagtgc ccaggctgat ctgactgtaa aggataagga cttgaataca
     5041 cccttacatt tggcttgtag taaaggtcat gaaaaatgtg ccttgttaat acttgacaag
     5101 atacaagacg agagccttat taatgaaaaa aataatgcac tgcagacacc cctccacgtc
     5161 gctgcgcgca atggcttaaa ggtggtagtt gaggagttgc tggccaaagg ggcctgtgta
     5221 cttgctgtag atgaaaatgc ttctaggtca aatggacccc gttccacacc tggaaccgct
     5281 gtacaaaaag aagaaggatc cgactacaaa gaccatgacg gtgattataa agatcatgac
     5341 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt
     5401 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     5461 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     5521 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     5581 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     5641 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     5701 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     5761 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     5821 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     5881 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg
     5941 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg
     6001 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg
     6061 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac
     6121 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc
     6181 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc
     6241 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc
     6301 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc
     6361 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc
     6421 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg
     6481 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc
     6541 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc
     6601 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac
     6661 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc
     6721 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc
     6781 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6841 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6901 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6961 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     7021 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     7081 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     7141 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     7201 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     7261 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     7321 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga
     7381 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7441 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7501 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7561 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7621 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7681 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7741 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7801 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7861 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7921 tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
     7981 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata
     8041 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8101 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8161 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8221 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     8281 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     8341 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8401 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     8461 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8521 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8581 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat
     8641 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8701 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8761 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8821 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8881 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8941 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     9001 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9061 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9121 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9181 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9241 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9301 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9361 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9421 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9481 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9541 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9601 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9661 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9721 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9781 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9841 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9901 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9961 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10021 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    10081 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    10141 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    10201 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10261 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10321 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10381 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10441 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10501 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10561 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10621 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10681 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10741 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10801 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
    10861 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    10921 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    10981 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
    11041 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    11101 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    11161 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    11221 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
