LOCUS       V043820                10673 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043820
VERSION     V043820
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3018
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3055..5598
                     /label="AHR(NM_001621)"
                     /note="AHR(NM_001621)"
                     /gene="AHR"
     promoter        5643..5854
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5867..6135
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6162..6761
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6768..7356
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7430..7663
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7735..7856
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7875..8010
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8048..8064)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8072..8088
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8096..8126)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8141..8162
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8450..9038)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9209..10069)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10070..10174)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10648..10664
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3001 atggacgagc tgtacaaggg cggcggaggg agtggaggag ggggaagtgg atccatgaac
     3061 agcagcagcg ccaacatcac ctacgccagt cgcaagcggc ggaagccggt gcagaaaaca
     3121 gtaaagccaa tcccagctga aggaatcaag tcaaatcctt ccaagcggca tagagaccga
     3181 cttaatacag agttggaccg tttggctagc ctgctgcctt tcccacaaga tgttattaat
     3241 aagttggaca aactttcagt tcttaggctc agcgtcagtt acctgagagc caagagcttc
     3301 tttgatgttg cattaaaatc ctcccctact gaaagaaacg gaggccagga taactgtaga
     3361 gcagcaaatt tcagagaagg cctgaactta caagaaggag aattcttatt acaggctctg
     3421 aatggctttg tattagttgt cactacagat gctttggtct tttatgcttc ttctactata
     3481 caagattatc tagggtttca gcagtctgat gtcatacatc agagtgtata tgaacttatc
     3541 cataccgaag accgagctga atttcagcgt cagctacact gggcattaaa tccttctcag
     3601 tgtacagagt ctggacaagg aattgaagaa gccactggtc tcccccagac agtagtctgt
     3661 tataacccag accagattcc tccagaaaac tctcctttaa tggagaggtg cttcatatgt
     3721 cgtctaaggt gtctgctgga taattcatct ggttttctgg caatgaattt ccaagggaag
     3781 ttaaagtatc ttcatggaca gaaaaagaaa gggaaagatg gatcaatact tccacctcag
     3841 ttggctttgt ttgcgatagc tactccactt cagccaccat ccatacttga aatccggacc
     3901 aaaaatttta tctttagaac caaacacaaa ctagacttca cacctattgg ttgtgatgcc
     3961 aaaggaagaa ttgttttagg atatactgaa gcagagctgt gcacgagagg ctcaggttat
     4021 cagtttattc atgcagctga tatgctttat tgtgccgagt cccatatccg aatgattaag
     4081 actggagaaa gtggcatgat agttttccgg cttcttacaa aaaacaaccg atggacttgg
     4141 gtccagtcta atgcacgcct gctttataaa aatggaagac cagattatat cattgtaact
     4201 cagagaccac taacagatga ggaaggaaca gagcatttac gaaaacgaaa tacgaagttg
     4261 ccttttatgt ttaccactgg agaagctgtg ttgtatgagg caaccaaccc ttttcctgcc
     4321 ataatggatc ccttaccact aaggactaaa aatggcacta gtggaaaaga ctctgctacc
     4381 acatccactc taagcaagga ctctctcaat cctagttccc tcctggctgc catgatgcaa
     4441 caagatgagt ctatttatct ctatcctgct tcaagtactt caagtactgc accttttgaa
     4501 aacaactttt tcaacgaatc tatgaatgaa tgcagaaatt ggcaagataa tactgcaccg
     4561 atgggaaatg atactatcct gaaacatgag caaattgacc agcctcagga tgtgaactca
     4621 tttgctggag gtcacccagg gctctttcaa gatagtaaaa acagtgactt gtacagcata
     4681 atgaaaaacc taggcattga ttttgaagac atcagacaca tgcagaatga aaaatttttc
     4741 agaaatgatt tttctggtga ggttgacttc agagacattg acttaacgga tgaaatcctg
     4801 acgtatgtcc aagattcttt aagtaagtct cccttcatac cttcagatta tcaacagcaa
     4861 cagtccttgg ctctgaactc aagctgtatg gtacaggaac acctacatct agaacagcaa
     4921 cagcaacatc accaaaagca agtagtagtg gagccacagc aacagctgtg tcagaagatg
     4981 aagcacatgc aagttaatgg catgtttgaa aattggaact ctaaccaatt cgtgcctttc
     5041 aattgtccac agcaagaccc acaacaatat aatgtcttta cagacttaca tgggatcagt
     5101 caagagttcc cctacaaatc tgaaatggat tctatgcctt atacacagaa ctttatttcc
     5161 tgtaatcagc ctgtattacc acaacattcc aaatgtacag agctggacta ccctatgggg
     5221 agttttgaac catccccata ccccactact tctagtttag aagattttgt cacttgttta
     5281 caacttcctg aaaaccaaaa gcatggatta aatccacagt cagccataat aactcctcag
     5341 acatgttatg ctggggccgt gtcgatgtat cagtgccagc cagaacctca gcacacccac
     5401 gtgggtcaga tgcagtacaa tccagtactg ccaggccaac aggcattttt aaacaagttt
     5461 cagaatggag ttttaaatga aacatatcca gctgaattaa ataacataaa taacactcag
     5521 actaccacac atcttcagcc acttcatcat ccgtcagaag ccagaccttt tcctgatttg
     5581 acatccagtg gattcctgta agcggccgcg aaggatctgc gatcgctccg gtgcccgtca
     5641 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     5701 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     5761 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     5821 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc
     5881 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct
     5941 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct
     6001 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct
     6061 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct
     6121 gcgccgttac agatccaagc tgtgaccggc gcctacgcta gatgaccgag tacaagccca
     6181 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
     6241 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
     6301 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     6361 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     6421 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     6481 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     6541 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     6601 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     6661 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     6721 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg agtcgacaat caacctctgg
     6781 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6841 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6901 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6961 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     7021 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     7081 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     7141 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     7201 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     7261 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     7321 agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag accaatgact
     7381 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7441 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac
     7501 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7561 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7621 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7681 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     7741 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7801 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7861 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7921 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7981 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt
     8041 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     8101 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8161 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8221 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8281 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8341 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8401 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8461 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8521 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8581 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8641 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8701 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8761 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8821 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8881 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8941 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9001 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9061 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9121 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9181 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9241 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9301 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9361 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9421 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9481 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9541 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9601 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9661 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9721 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9781 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9841 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9901 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9961 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10021 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10081 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10141 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    10201 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    10261 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    10321 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    10381 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
    10441 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    10501 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    10561 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    10621 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
