LOCUS V036008 10990 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V036008 VERSION V036008 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..5844 /label="ADAR(NM_015841)" /note="ADAR(NM_015841)" /gene="ADAR" CDS 5851..5916 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 5960..6171 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 6184..6452 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 6479..7078 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7085..7673 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7747..7980 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 8052..8173 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 8192..8327 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(8365..8381) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8389..8405 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8413..8443) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8458..8479 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8767..9355) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9526..10386) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10387..10491) /gene="bla" /label="AmpR promoter" primer_bind 10965..10981 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgaatccg cggcaggggt attccctcag cggatactac 2341 acccatccat ttcaaggcta tgagcacaga cagctcaggt accagcagcc tgggccagga 2401 tcttccccca gtagtttcct gcttaagcaa atagaatttc tcaaggggca gctcccagaa 2461 gcaccggtga ttggaaagca gacaccgtca ctgccacctt ccctcccagg actccggcca 2521 aggtttccag tactacttgc ctccagtacc agaggcaggc aagtggacat caggggtgtc 2581 cccaggggcg tgcatctcag aagtcagggg ctccagagag ggttccagca tccttcacca 2641 cgtggcagga gtctgccaca gagaggtgtt gattgccttt cctcacattt ccaggaactg 2701 agtatctacc aagatcagga acaaaggatc ttaaagttcc tggaagagct tggggaaggg 2761 aaggccacca cagcacatga tctgtctggg aaacttggga ctccgaagaa agaaatcaat 2821 cgagttttat actccctggc aaagaagggc aagctacaga aagaggcagg aacaccccct 2881 ttgtggaaaa tcgcggtctc cactcaggct tggaaccagc acagcggagt ggtaagacca 2941 gacggtcata gccaaggagc cccaaactca gacccgagtt tggaaccgga agacagaaac 3001 tccacatctg tctcagaaga tcttcttgag ccttttattg cagtctcagc tcaggcttgg 3061 aaccagcaca gcggagtggt aagaccagac agtcatagcc aaggatcccc aaactcagac 3121 ccaggtttgg aacctgaaga cagcaactcc acatctgcct tggaagatcc tcttgagttt 3181 ttagacatgg ccgagatcaa ggagaaaatc tgcgactatc tcttcaatgt gtctgactcc 3241 tctgccctga atttggctaa aaatattggc cttaccaagg cccgagatat aaatgctgtg 3301 ctaattgaca tggaaaggca gggggatgtc tatagacaag ggacaacccc tcccatatgg 3361 catttgacag acaagaagcg agagaggatg caaatcaaga gaaatacgaa cagtgttcct 3421 gaaaccgctc cagctgcaat ccctgagacc aaaagaaacg cagagttcct cacctgtaat 3481 atacccacat caaatgcctc aaataacatg gtaaccacag aaaaagtgga gaatgggcag 3541 gaacctgtca taaagttaga aaacaggcaa gaggccagac cagaaccagc aagactgaaa 3601 ccacctgttc attacaatgg cccctcaaaa gcagggtatg ttgactttga aaatggccag 3661 tgggccacag atgacatccc agatgacttg aatagtatcc gcgcagcacc aggtgagttt 3721 cgagccatca tggagatgcc ctccttctac agtcatggct tgccacggtg ttcaccctac 3781 aagaaactga cagagtgcca gctgaagaac cccatcagcg ggctgttaga atatgcccag 3841 ttcgctagtc aaacctgtga gttcaacatg atagagcaga gtggaccacc ccatgaacct 3901 cgatttaaat tccaggttgt catcaatggc cgagagtttc ccccagctga agctggaagc 3961 aagaaagtgg ccaagcagga tgcagctatg aaagccatga caattctgct agaggaagcc 4021 aaagccaagg acagtggaaa atcagaagaa tcatcccact attccacaga gaaagaatca 4081 gagaagactg cagagtccca gacccccacc ccttcagcca catccttctt ttctgggaag 4141 agccccgtca ccacactgct tgagtgtatg cacaaattgg ggaactcctg cgaattccgt 4201 ctcctgtcca aagaaggccc tgcccatgaa cccaagttcc aatactgtgt tgcagtggga 4261 gcccaaactt tccccagtgt gagtgctccc agcaagaaag tggcaaagca gatggccgca 4321 gaggaagcca tgaaggccct gcatggggag gcgaccaact ccatggcttc tgataaccag 4381 gtcaggaaga ttggcgagct cgtgagatac ctgaacacca accctgtggg tggccttttg 4441 gagtacgccc gctcccatgg ctttgctgct gaattcaagt tggtcgacca gtccggacct 4501 cctcacgagc ccaagttcgt ttaccaagca aaagttgggg gtcgctggtt cccagccgtc 4561 tgcgcacaca gcaagaagca aggcaagcag gaagcagcag atgcggctct ccgtgtcttg 4621 attggggaga acgagaaggc agaacgcatg ggtttcacag agctccctct cactggcagc 4681 accttccatg accagatagc catgctgagc caccggtgct tcaacactct gactaacagc 4741 ttccagccct ccttgctcgg ccgcaagatt ctggccgcca tcattatgaa aaaagactct 4801 gaggacatgg gtgtcgtcgt cagcttggga acagggaatc gctgtgtgaa aggagattct 4861 ctcagcctaa aaggagaaac tgtcaatgac tgccatgcag aaataatctc ccggagaggc 4921 ttcatcaggt ttctctacag tgagttaatg aaatacaact cccagactgc gaaggatagt 4981 atatttgaac ctgctaaggg aggagaaaag ctccaaataa aaaagactgt gtcattccat 5041 ctgtatatca gcactgctcc gtgtggagat ggcgccctct ttgacaagtc ctgcagcgac 5101 cgtgctatgg aaagcacaga atcccgccac taccctgtct tcgagaatcc caaacaagga 5161 aagctccgca ccaaggtgga gaacggagaa ggcacaatcc ctgtggaatc cagtgacatt 5221 gtgcctacgt gggatggcat tcggctcggg gagagactcc gtaccatgtc ctgtagtgac 5281 aaaatcctac gctggaacgt gctgggcctg caaggggcac tgttgaccca cttcctgcag 5341 cccatttatc tcaaatctgt cacattgggt taccttttca gccaagggca tctgacccgt 5401 gctatttgct gtcgtgtgac aagagatggg agtgcatttg aggatggact acgacatccc 5461 tttattgtca accaccccaa ggttggcaga gtcagcatat atgattccaa aaggcaatcc 5521 gggaagacta aggagacaag cgtcaactgg tgtctggctg atggctatga cctggagatc 5581 ctggacggta ccagaggcac tgtggatggg ccacggaatg aattgtcccg ggtctccaaa 5641 aagaacattt ttcttctatt taagaagctc tgctccttcc gttaccgcag ggatctactg 5701 agactctcct atggtgaggc caagaaagct gcccgtgact acgagacggc caagaactac 5761 ttcaaaaaag gcctgaagga tatgggctat gggaactgga ttagcaaacc ccaggaggaa 5821 aagaactttt atctctgccc agtaggatcc gactacaaag accatgacgg tgattataaa 5881 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat 5941 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 6001 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 6061 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 6121 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt 6181 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 6241 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 6301 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 6361 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 6421 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagat 6481 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg 6541 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg 6601 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 6661 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 6721 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 6781 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 6841 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 6901 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 6961 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 7021 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt 7081 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 7141 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 7201 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 7261 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 7321 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 7381 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 7441 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct 7501 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 7561 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 7621 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac 7681 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 7741 ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg 7801 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 7861 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 7921 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 7981 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 8041 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 8101 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 8161 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc 8221 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 8281 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag 8341 agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 8401 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 8461 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 8521 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 8581 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 8641 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 8701 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 8761 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 8821 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 8881 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 8941 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 9001 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 9061 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 9121 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 9181 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 9241 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 9301 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 9361 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 9421 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 9481 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 9541 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 9601 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 9661 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 9721 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 9781 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 9841 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 9901 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 9961 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 10021 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 10081 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 10141 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 10201 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 10261 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 10321 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 10381 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 10441 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 10501 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 10561 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 10621 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 10681 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 10741 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 10801 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 10861 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 10921 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 10981 tgccaagctg //