LOCUS       V044840                10201 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044840
VERSION     V044840
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5055
                     /label="ADAM19(NM_033274)"
                     /note="ADAM19(NM_033274)"
                     /gene="ADAM19"
     CDS             5062..5127
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5171..5382
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5395..5663
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5690..6289
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6296..6884
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6958..7191
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7263..7384
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7403..7538
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7576..7592)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7600..7616
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7624..7654)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7669..7690
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7978..8566)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8737..9597)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9598..9702)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10176..10192
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgccaggg ggcgcaggcg ccgcccggct ctgcttgctg
     2341 gcgtttgccc tgcagcccct ccggccgcgg gcggcgcggg agcctggatg gacaagagga
     2401 agtgaggaag gcagccccaa gctgcagcat gaacttatca tacctcagtg gaagacttca
     2461 gaaagccccg tgagagaaaa gcatccactc aaagctgagc tcagggtaat ggctgagggg
     2521 cgagaactga tcctggacct ggagaagaat gagcaacttt ttgctccttc ctacacagaa
     2581 acccattata cttcaagtgg taaccctcaa accaccacac ggaaattgga ggatcactgc
     2641 ttttaccacg gcacggtgag ggagacagaa ctgtccagcg tcacgctcag cacttgccga
     2701 ggaattagag gactgattac ggtgagcagc aacctcagct acgtcatcga gcccctccct
     2761 gacagcaagg gccaacacct tatttacaga tctgaacatc tcaagccgcc cccgggaaac
     2821 tgtgggttcg agcactccaa gcccaccacc agggactggg ctcttcagtt tacacaacag
     2881 accaagaagc gacctcgcag gatgaaaagg gaagatttaa actccatgaa gtatgtggag
     2941 ctttacctcg tggctgatta tttagagttt cagaagaatc gacgagacca ggacgccacc
     3001 aaacacaagc tcatagagat cgccaactat gttgataagt tttaccgatc cttgaacatc
     3061 cggattgctc tcgtgggctt ggaagtgtgg acccacggga acatgtgtga agtttcagag
     3121 aatccatatt ctaccctctg gtcctttctc agttggaggc gcaagctgct tgcccagaag
     3181 taccatgaca acgcccaatt aatcacgggc atgtccttcc acggcaccac catcggcctg
     3241 gcccccctca tggccatgtg ctctgtgtac cagtctggag gagtcaacat ggaccactcc
     3301 gagaatgcca ttggcgtggc tgccaccatg gcccacgaga tgggccacaa ctttggcatg
     3361 acccatgatt ctgcagattg ctgctcggcc agtgcggctg atggtgggtg catcatggca
     3421 gctgccactg ggcacccctt tcccaaagtg ttcaatggat gcaacaggag ggagctggac
     3481 aggtatctgc agtcaggtgg tggaatgtgt ctctccaaca tgccagacac caggatgttg
     3541 tatggaggcc ggaggtgtgg gaacgggtat ctggaagatg gggaagagtg tgactgtgga
     3601 gaagaagagg aatgtaacaa cccctgctgc aatgcctcta attgtaccct gaggccgggg
     3661 gcggagtgtg ctcacggctc ctgctgccac cagtgtaagc tgttggctcc tgggaccctg
     3721 tgccgcgagc aggccaggca gtgtgacctc ccggagttct gtacgggcaa gtctccccac
     3781 tgccctacca acttctacca gatggatggt accccctgtg agggcggcca ggcctactgc
     3841 tacaacggca tgtgcctcac ctaccaggag cagtgccagc agctgtgggg acccggagcc
     3901 cgacctgccc ctgacctctg cttcgagaag gtgaatgtgg caggagacac ctttggaaac
     3961 tgtggaaagg acatgaatgg tgaacacagg aagtgcaaca tgagagatgc gaagtgtggg
     4021 aagatccagt gtcagagctc tgaggcccgg cccctggagt ccaacgcggt gcccattgac
     4081 accactatca tcatgaatgg gaggcagatc cagtgccggg gcacccacgt ctaccgaggt
     4141 cctgaggagg agggtgacat gctggaccca gggctggtga tgactggaac caagtgtggc
     4201 tacaaccata tttgctttga ggggcagtgc aggaacacct ccttctttga aactgaaggc
     4261 tgtgggaaga agtgcaatgg ccatggggtc tgtaacaaca accagaactg ccactgcctg
     4321 ccgggctggg ccccgccctt ctgcaacaca ccgggccacg ggggcagtat cgacagtggg
     4381 cctatgcccc ctgagagtgt gggtcctgtg gtagctggag tgttggtggc catcttggtg
     4441 ctggcggtcc tcatgctgat gtactactgc tgcagacaga acaacaaact aggccaactc
     4501 aagccctcag ctctcccttc caagctgagg caacagttca gttgtccctt cagggtttct
     4561 cagaacagcg ggactggtca tgccaaccca actttcaagc tgcagacgcc ccagggcaag
     4621 cgaaaggtga tcaacactcc ggaaatcctg cggaagccct cccagcctcc tccccggccc
     4681 cctccagatt atctgcgtgg tgggtcccca cctgcaccac tgccagctca cctgagcagg
     4741 gctgctagga actccccagg gcccgggtct caaatagaga ggacggagtc gtccaggagg
     4801 cctcctccaa gccggccaat tccccccgca ccaaattgca tcgtttccca ggacttctcc
     4861 aggcctcggc cgccccagaa ggcactcccg gcaaacccag tgccaggccg caggagcctc
     4921 cccaggccag gaggtgcatc cccactgcgg ccccctggtg ctggccctca gcagtcccgg
     4981 cctctggcag cacttgcccc aaagtttcca gaatacagat cacagagggc tggagggatg
     5041 attagctcga aaatcggatc cgactacaaa gaccatgacg gtgattataa agatcatgac
     5101 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt
     5161 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     5221 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     5281 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     5341 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     5401 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     5461 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     5521 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     5581 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     5641 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga tgaccgagta
     5701 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
     5761 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
     5821 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     5881 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     5941 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     6001 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6061 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6121 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     6181 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6241 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca
     6301 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6361 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     6421 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     6481 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6541 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6601 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6661 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     6721 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6781 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6841 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac
     6901 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6961 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct
     7021 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7081 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7141 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     7201 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     7261 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7321 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7381 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc
     7441 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7501 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc
     7561 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7621 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7681 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7741 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7801 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7861 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7921 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7981 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8041 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8101 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8161 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8221 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8281 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8341 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8401 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8461 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8521 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8581 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8641 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8701 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8761 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8821 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8881 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8941 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9001 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9061 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9121 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9181 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9241 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9301 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9361 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9421 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9481 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9541 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9601 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9661 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9721 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     9781 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     9841 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     9901 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     9961 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    10021 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    10081 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    10141 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    10201 g
//
