LOCUS       V038066                10242 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038066
VERSION     V038066
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4261
                     /label="ACADVL(NM_000018)"
                     /note="ACADVL(NM_000018)"
                     /gene="ACADVL"
     CDS             4268..4357
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        4401..4612
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4625..4893
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4922..4931
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4952..5614
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5684..5737
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5738..6337
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6338..6925
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6999..7232
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7304..7425
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7444..7579
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7617..7633)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7641..7657
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7665..7695)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7710..7731
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8019..8607)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8778..9638)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9639..9743)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10217..10233
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgc aggcggctcg gatggccgcg agcttggggc ggcagctgct
     2341 gaggctcggg ggcggaagct cgcggctcac ggcgctcctg gggcagcccc ggcccggccc
     2401 tgcccggcgg ccctatgccg ggggtgccgc tcagctggct ctggacaagt cagattccca
     2461 cccctctgac gctctgacca ggaaaaaacc ggccaaggcg gaatctaagt cctttgctgt
     2521 gggaatgttc aaaggccagc tcaccacaga tcaggtgttc ccatacccgt ccgtgctcaa
     2581 cgaagagcag acacagtttc ttaaagagct ggtggagcct gtgtcccgtt tcttcgagga
     2641 agtgaacgat cccgccaaga atgacgctct ggagatggtg gaggagacca cttggcaggg
     2701 cctcaaggag ctgggggcct ttggtctgca agtgcccagt gagctgggtg gtgtgggcct
     2761 ttgcaacacc cagtacgccc gtttggtgga gatcgtgggc atgcatgacc ttggcgtggg
     2821 cattaccctg ggggcccatc agagcatcgg tttcaaaggc atcctgctct ttggcacaaa
     2881 ggcccagaaa gaaaaatacc tccccaagct ggcatctggg gagactgtgg ccgctttctg
     2941 tctaaccgag ccctcaagcg ggtcagatgc agcctccatc cgaacctctg ctgtgcccag
     3001 cccctgtgga aaatactata ccctcaatgg aagcaagctt tggatcagta atgggggcct
     3061 agcagacatc ttcacggtct ttgccaagac accagttaca gatccagcca caggagccgt
     3121 gaaggagaag atcacagctt ttgtggtgga gaggggcttc gggggcatta cccatgggcc
     3181 ccctgagaag aagatgggca tcaaggcttc aaacacagca gaggtgttct ttgatggagt
     3241 acgggtgcca tcggagaacg tgctgggtga ggttgggagt ggcttcaagg ttgccatgca
     3301 catcctcaac aatggaaggt ttggcatggc tgcggccctg gcaggtacca tgagaggcat
     3361 cattgctaag gcggtagatc atgccactaa tcgtacccag tttggggaga aaattcacaa
     3421 ctttgggctg atccaggaga agctggcacg gatggttatg ctgcagtatg taactgagtc
     3481 catggcttac atggtgagtg ctaacatgga ccagggagcc acggacttcc agatagaggc
     3541 cgccatcagc aaaatctttg gctcggaggc agcctggaag gtgacagatg aatgcatcca
     3601 aatcatgggg ggtatgggct tcatgaagga acctggagta gagcgtgtgc tccgagatct
     3661 tcgcatcttc cggatctttg aggggacaaa tgacattctt cggctgtttg tggctctgca
     3721 gggctgtatg gacaaaggaa aggagctctc tgggcttggc agtgctctaa agaatccctt
     3781 tgggaatgct ggcctcctgc taggagaggc aggcaaacag ctgaggcggc gggcagggct
     3841 gggcagcggc ctgagtctca gcggacttgt ccacccggag ttgagtcgga gtggcgagct
     3901 ggcagtacgg gctctggagc agtttgccac tgtggtggag gccaagctga taaaacacaa
     3961 gaaggggatt gtcaatgaac agtttctgct gcagcggctg gcagacgggg ccatcgacct
     4021 ctatgccatg gtggtggttc tctcgagggc ctcaagatcc ctgagtgagg gccaccccac
     4081 ggcccagcat gagaaaatgc tctgtgacac ctggtgtatc gaggctgcag ctcggatccg
     4141 agagggcatg gccgccctgc agtctgaccc ctggcagcaa gagctctacc gcaacttcaa
     4201 aagcatctcc aaggccttgg tggagcgggg tggtgtggtc accagcaacc cacttggctt
     4261 cggatccgaa caaaaactaa tatcagagga agatttggaa cagaaactaa tcagtgaaga
     4321 agatttagag caaaagttaa tttccgagga ggacttatag cggccgcgaa ggatctgcga
     4381 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     4441 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     4501 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     4561 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     4621 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     4681 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     4741 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     4801 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     4861 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga
     4921 cgccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac
     4981 cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca
     5041 gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct
     5101 gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga
     5161 gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta
     5221 cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat
     5281 cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa
     5341 gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt
     5401 gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga
     5461 cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat
     5521 gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta
     5581 ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc
     5641 tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct
     5701 tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt
     5761 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     5821 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     5881 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     5941 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6001 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6061 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6121 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6181 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     6241 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     6301 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat
     6361 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     6421 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     6481 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     6541 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6601 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6661 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6721 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     6781 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6841 cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg
     6901 gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     6961 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7021 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7081 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7141 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7201 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     7261 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     7321 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7381 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     7441 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7501 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7561 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca
     7621 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7681 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7741 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7801 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7861 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7921 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7981 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8041 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8101 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8161 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8221 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8281 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8341 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8401 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     8461 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8521 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8581 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8641 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8701 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8761 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8821 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8881 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8941 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9001 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9061 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9121 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9181 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9241 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9301 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9361 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9421 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9481 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9541 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9601 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9661 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9721 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     9781 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     9841 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     9901 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     9961 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
    10021 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
    10081 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    10141 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    10201 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
