LOCUS       V037929                10351 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037929
VERSION     V037929
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5205
                     /label="AARS1(NM_001605)"
                     /note="AARS1(NM_001605)"
                     /gene="AARS1"
     CDS             5212..5277
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5321..5532
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5545..5813
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5840..6439
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6446..7034
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7108..7341
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7413..7534
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7553..7688
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7726..7742)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7750..7766
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7774..7804)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7819..7840
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8128..8716)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8887..9747)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9748..9852)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10326..10342
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggactct actctaacag caagtgaaat ccggcagcga
     2341 tttatagatt tcttcaagag gaacgagcat acgtatgttc actcgtctgc caccatccca
     2401 ttggatgacc ccactttgct ctttgccaat gcaggcatga accagtttaa acccattttc
     2461 ctgaacacaa ttgacccatc tcaccccatg gcaaagctga gcagagctgc caatacccag
     2521 aagtgcatcc gggctggggg caaacataat gacctggacg atgtgggcaa ggatgtctat
     2581 catcacacct tcttcgagat gctgggctct tggtcttttg gagattactt taaggaattg
     2641 gcatgtaaga tggctctgga actcctcacc caagagtttg gcattcccat tgaaagactt
     2701 tatgttactt actttggcgg ggatgaagca gctggcttag aagcagatct ggaatgcaaa
     2761 cagatctggc aaaatttggg gctggatgac accaaaatcc tcccaggcaa catgaaggat
     2821 aacttctggg agatgggtga cacgggcccc tgtggtcctt gcagtgagat ccactacgac
     2881 cggattggtg gtcgggacgc cgcacatctt gtcaaccagg acgaccctaa tgtgctggag
     2941 atctggaacc ttgtgttcat ccagtataac agggaagctg atggcattct gaaacctctt
     3001 cccaagaaaa gcattgacac agggatgggc ctggaacgac tggtatctgt gctgcagaat
     3061 aagatgtcca actatgacac tgaccttttt gtcccttact ttgaagccat tcagaagggc
     3121 acaggtgccc gaccatacac tgggaaagtt ggtgctgagg atgccgatgg gattgacatg
     3181 gcctaccggg tgctggctga ccacgctcgg accatcactg tggcactggc tgatggtggc
     3241 cggcctgaca acacagggcg tggatatgtg ttgagacgga ttctccgccg agctgtccga
     3301 tacgcccatg aaaagctcaa tgccagcagg ggcttctttg ctacgttagt ggatgttgtc
     3361 gtccagtccc tgggagatgc atttcctgag ctgaagaagg acccagacat ggtgaaggac
     3421 atcattaatg aagaagaggt gcagtttctc aagactctca gcagagggcg tcgcatcctg
     3481 gacaggaaaa ttcagagcct gggagacagc aagaccattc ccggagacac tgcttggctc
     3541 ctctatgaca cctatgggtt tccagtggat ctgactggac tgattgctga agagaagggc
     3601 ctggtggtag acatggatgg ctttgaagag gagaggaaac tggcccagct gaaatcacag
     3661 ggcaagggag ctggtgggga agacctcatt atgctggaca tttacgctat cgaagagctc
     3721 cgggcacggg gtctggaggt cacagatgat tccccaaagt acaattacca tttggactcc
     3781 agtggtagct atgtatttga gaacacagtg gctacggtga tggctctgcg cagggagaag
     3841 atgttcgtgg aagaggtgtc cacaggccag gagtgtggag tggtgctgga caagacctgt
     3901 ttctatgctg agcaaggagg ccagatctat gacgaaggct acctggtgaa ggtggatgac
     3961 agcagtgaag ataaaacaga gtttacagtg aagaatgctc aggtccgagg agggtatgtg
     4021 ctacacattg gaaccatcta cggtgacctg aaagtggggg atcaggtctg gctgtttatt
     4081 gatgagcccc gacgaagacc catcatgagc aaccacacag ctacgcacat tctgaacttc
     4141 gccctgcgct cagtgcttgg ggaagctgac cagaaaggct cattggttgc tcctgaccgc
     4201 ctcagatttg actttactgc caagggagcc atgtccaccc aacagatcaa gaaggctgaa
     4261 gagattgcta atgagatgat tgaggcagcc aaggccgtct atacccagga ttgccccctg
     4321 gcagcagcga aagccatcca gggcctacgg gctgtgtttg atgagaccta tcctgaccct
     4381 gtgcgagtcg tctccattgg ggtcccggtg tccgagttgc tggatgaccc ctctgggcct
     4441 gctggctccc tgacttctgt tgagttctgt gggggaacgc acctgcggaa ctcgagtcat
     4501 gcaggagctt ttgtgatcgt gacggaagaa gccattgcca agggtatccg gaggattgtg
     4561 gctgtcacag gtgccgaggc ccagaaggcc ctcaggaaag cagagagctt gaagaaatgt
     4621 ctctctgtca tggaagccaa agtgaaggct cagactgctc caaacaagga tgtgcagagg
     4681 gagatcgctg accttggaga ggccctggcc actgcagtca tcccccagtg gcagaaggat
     4741 gaattgcggg agactctcaa atccctaaag aaggtcatgg atgacttgga ccgagccagc
     4801 aaagccgatg tccagaaacg agtgttagag aagacgaagc agttcatcga cagcaacccc
     4861 aaccagcctc ttgtcatcct ggagatggag agcggcgcct cagccaaggc cctgaatgaa
     4921 gccttgaagc tcttcaagat gcactcccct cagacttctg ccatgctctt cacggtggac
     4981 aatgaggctg gcaagatcac gtgcctgtgt caagttcccc agaatgcagc caatcggggc
     5041 ttaaaagcca gcgagtgggt gcagcaggtg tcaggcttga tggacggtaa aggtggtggc
     5101 aaggatgtgt ctgcacaggc cacaggcaag aacgttggct gcctgcagga ggcgctgcag
     5161 ctggccactt ccttcgccca gctgcgcctc ggggatgtaa agaacggatc cgactacaaa
     5221 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtag
     5281 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     5341 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     5401 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     5461 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     5521 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     5581 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     5641 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     5701 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     5761 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     5821 tgaccggcgc ctacgctaga tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     5881 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     5941 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     6001 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     6061 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     6121 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     6181 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     6241 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     6301 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     6361 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     6421 ccgcaagccc ggtgcctgag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     6481 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6541 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6601 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6661 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6721 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6781 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6841 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6901 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6961 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7021 ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta
     7081 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag
     7141 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7201 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7261 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7321 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     7381 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     7441 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7501 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     7561 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7621 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7681 tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc
     7741 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7801 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7861 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7921 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7981 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8041 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8101 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8161 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8221 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8281 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8341 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8401 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8461 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8521 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     8581 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8641 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8701 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8761 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8821 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8881 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8941 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9001 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9061 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9121 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9181 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9241 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9301 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9361 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9421 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9481 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9541 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9601 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9661 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9721 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9781 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9841 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     9901 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     9961 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
    10021 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
    10081 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
    10141 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
    10201 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    10261 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
    10321 acgttgtaaa acgacggcca gtgccaagct g
//
