LOCUS       .                       9120 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    458..583
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1804..1920
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2115..2146
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             2179..3606
                     /label="tdTomato"
                     /note="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
     CDS             3853..4563
                     /label="mTagBFP2"
                     /note="enhanced monomeric blue fluorescent protein (Subach
                     et al., 2011)"
     promoter        4574..4785
                     /label="EF-1α core promoter"
                     /note="core promoter for human elongation factor EF-1α"
     CDS             4810..5205
                     /label="BSD"
                     /note="blasticidin S deaminase"
     misc_feature    5215..5803
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5877..6110
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6182..6316
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6322..6457
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(6495..6511)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6519..6535)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6543..6573)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6588..6609)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6897..7485)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7659..8516)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(8517..8621)
                     /label="AmpR promoter"
     primer_bind     9095..9111
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgc tcgagtggat ccaaaccgaa actaacgtta
     2041 taccgaaacc aacgttaaac cgaaactaac gttataccga aaccaagaat tcagatctgc
     2101 agaagcttag acactagagg gtatataatg gaagctcgac ttccagcttg gcaatccggt
     2161 actgttggta aagccaccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc
     2221 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     2281 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     2341 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
     2401 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
     2461 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
     2521 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
     2581 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
     2641 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
     2701 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
     2761 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     2821 tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcatcgg cagcaccggc
     2881 agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc
     2941 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     3001 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     3061 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     3121 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     3181 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     3241 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     3301 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     3361 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     3421 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     3481 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     3541 gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg
     3601 tacaagtgag ccacgcggcc gcattatgcc cagtacatga ccttatggga ctttcctact
     3661 tggcagtaca tctacgtatt agtcatcgct attaccatgc tcgagttagt ttcactttcc
     3721 ctagtttcac tttccctagt ttcactttcc ctagtttcac tttcccagat ctgcagaagc
     3781 ttagacacta gagggtatat aatggaagct cgacttccag cttggcaatc cggtactgtt
     3841 ggtaaagcca ccatggtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag
     3901 ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc
     3961 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc
     4021 ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac
     4081 acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga
     4141 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac
     4201 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg
     4261 atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc
     4321 ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca
     4381 aacgccaaga ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc
     4441 tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag
     4501 cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt
     4561 aattgacgtc gacgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     4621 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     4681 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     4741 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaacc
     4801 ggtgccacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg
     4861 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct
     4921 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca
     4981 gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc
     5041 gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt
     5101 ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca
     5161 gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagt cgacaatcaa
     5221 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5281 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5341 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5401 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5461 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     5521 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     5581 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     5641 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     5701 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     5761 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     5821 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5881 agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg
     5941 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6001 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6061 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     6121 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     6181 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6241 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6301 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg
     6361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6421 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca
     6481 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6541 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6601 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6661 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6721 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6781 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6841 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6901 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6961 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7021 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7081 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7141 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7201 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7261 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7321 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7381 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7441 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7501 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7561 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7621 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7681 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7741 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7801 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7861 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7921 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7981 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8041 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8101 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8161 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8221 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8281 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8341 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8401 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8461 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8521 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8581 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8641 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     8701 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     8761 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     8821 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     8881 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     8941 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     9001 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     9061 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
