LOCUS       V014147                10107 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014147
VERSION     V014147
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    241..474
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             659..703
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             852..893
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1001..1118
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    1163..1183
                     /label="attB4"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     promoter        1215..1455
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        1465..1494
                     /label="RfxCas13d DR30"
                     /note="5' processed 30-base direct repeat for an RfxCas13d
                     gRNA (Konermann et al., 2018)"
     promoter        1595..2007
                     /label="cTnT"
                     /note="Chicken cardiac troponin T promoter"
     protein_bind    2064..2088
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2134..2154
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2173..3201
                     /label="Cre"
                     /note="site-specific recombinase"
     CDS             3220..3267
                     /label="nucleoplasmin NLS"
                     /note="bipartite nuclear localization signal from
                     nucleoplasmin"
     protein_bind    complement(3302..3326)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3360..3948
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             4473..4653
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4685..4909
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4955..5383
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5397..5726
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        5774..5821
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5840..6211
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    6344..6477
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(6562..6592)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6607..6628)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6916..7504)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7678..8535)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8536..8640)
                     /label="AmpR promoter"
     enhancer        8906..9285
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9286..9488
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    9730..9855
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
ORIGIN
        1 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
       61 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
      121 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
      181 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
      241 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
      301 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      361 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      421 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      481 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      541 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      601 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      661 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      721 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      781 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      841 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      901 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
      961 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1021 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1081 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1141 gagatccagt ttggttaatt aacaactttg tatagaaaag ttggctccga atttctcgag
     1201 gaattcggca ggaagagggc ctatttccca tgattccttc atatttgcat atacgataca
     1261 aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa
     1321 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     1381 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     1441 tcttgtggaa aggacgaaac accgaacccc taccaactgg tcggggtttg aaactgagac
     1501 catcggtctc atttttttaa gcttatagta atcaattacg gggtcattag ttcatagccc
     1561 atatatggag ttccgcgggc cccccctcga ggtcgggata aaagcagtct gggctttcac
     1621 atgacagcat ctggggctgc ggcagagggt cgggtccgaa gcgctgcctt atcagcgtcc
     1681 ccagccctgg gaggtgacag ctggctggct tgtgtcagcc cctcgggcac tcacgtatct
     1741 ccgtccgacg ggtttaaaat agcaaaactc tgaggccaca caatagcttg ggcttatatg
     1801 ggctcctgtg ggggaagggg gagcacggag ggggccgggg ccgctgctgc caaaatagca
     1861 gctcacaagt gttgcattcc tctctgggcg ccgggcacat tcctgctggc tctgcccgcc
     1921 ccggggtggg cgccgggggg accttaaagc ctctgccccc caaggagccc ttcccagaca
     1981 gccgccggca cccaccgctc cgtgggacga tccccgaggt ttagtgaacc gtcagatccc
     2041 cgggtaactg atcataattc gacccaagtt tgtacaaaaa agcaggctga ttaccggaga
     2101 attccaattg gcggccgcac cggtgccacc atgccaaaga agaagcggaa ggtcggtggc
     2161 ggctcacccg ggatgtccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc
     2221 gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg
     2281 ttttctgagc atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc
     2341 aagttgaata accggaaatg gtttcccgca gaacctgaag atgttcgcga ttatcttcta
     2401 tatcttcagg cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac
     2461 atgcttcatc gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt
     2521 atgcggcgga tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg
     2581 ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg aaaatagcga tcgctgccag
     2641 gatatacgta atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa
     2701 attgccagga tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat
     2761 attggcagaa cgaaaacgct ggttagcacc gcaggtgtag agaaggcact tagcctgggg
     2821 gtaactaaac tggtcgagcg atggatttcc gtctctggtg tagctgatga tccgaataac
     2881 tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc catctgccac cagccagcta
     2941 tcaactcgcg ccctggaagg gatttttgaa gcaactcatc gattgattta cggcgctaag
     3001 gatgactctg gtcagagata cctggcctgg tctggacaca gtgcccgtgt cggagccgcg
     3061 cgagatatgg cccgcgctgg agtttcaata ccggagatca tgcaagctgg tggctggacc
     3121 aatgtaaata ttgtcatgaa ctatatccgt aacctggata gtgaaacagg ggcaatggtg
     3181 cgcctgctgg aagatggcga tactagtggt ggcggctcaa agcgtcctgc tgctactaag
     3241 aaagctggtc aagctaagaa aaagaaatct agatagtgac tcgagactag ataactgatc
     3301 tacccagctt tcttgtacaa agtggtgaat tccggcaatt cgatatcaag cttatcgata
     3361 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3421 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3481 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3541 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3601 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3661 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3721 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     3781 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3841 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3901 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     3961 gacctcgagt cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     4021 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     4081 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4141 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     4201 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga
     4261 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg
     4321 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga
     4381 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc
     4441 acatggcccg agagctgcat ccggactgta ctgggtgtct ctggttagac cagatctgag
     4501 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4561 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4621 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc
     4681 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     4741 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     4801 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     4861 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     4921 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     4981 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5041 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5101 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5161 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5221 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5281 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5341 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     5401 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5461 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     5521 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     5581 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5641 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     5701 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     5761 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     5821 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
     5881 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
     5941 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
     6001 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
     6061 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
     6121 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
     6181 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
     6241 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6301 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     6361 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6421 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     6481 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6541 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6601 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6661 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6721 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6781 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6841 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6901 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6961 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7021 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7081 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7141 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7201 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7261 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7321 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7381 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7441 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7501 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7561 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7621 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7681 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7741 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7801 tgcaatgata ccgcgtgacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7861 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7921 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7981 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8041 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8101 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8161 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8221 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8281 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8341 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8401 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8461 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8521 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8581 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8641 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     8701 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     8761 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     8821 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     8881 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     8941 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     9001 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     9061 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     9121 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     9181 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     9241 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     9301 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     9361 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     9421 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     9481 agcagcgcgt tttgcctgta ctgggtgtct ctggttagac cagatctgag cctgggagct
     9541 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     9601 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     9661 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     9721 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     9781 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     9841 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     9901 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     9961 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
    10021 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
    10081 acagtagcaa ccctctattg tgtgcat
//
