LOCUS       V014145                10173 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014145
VERSION     V014145
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10173)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    241..474
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             659..703
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             852..893
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1001..1118
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    1163..1183
                     /label="attB4"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     promoter        1215..1455
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        1465..1494
                     /label="RfxCas13d DR30"
                     /note="5' processed 30-base direct repeat for an RfxCas13d
                     gRNA (Konermann et al., 2018)"
     promoter        1880..2083
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    2130..2154
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2200..2220
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2239..3267
                     /label="Cre"
                     /note="site-specific recombinase"
     CDS             3286..3333
                     /label="nucleoplasmin NLS"
                     /note="bipartite nuclear localization signal from
                     nucleoplasmin"
     protein_bind    complement(3368..3392)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3426..4014
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    4751..4975
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5021..5449
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5463..5792
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        5840..5887
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5906..6277
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    6410..6543
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(6628..6658)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6673..6694)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6982..7570)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7744..8601)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8602..8706)
                     /label="AmpR promoter"
     enhancer        8972..9351
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             9569..9749
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9796..9921
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
ORIGIN
        1 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
       61 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
      121 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
      181 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
      241 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
      301 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      361 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      421 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      481 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      541 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      601 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      661 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      721 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      781 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      841 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      901 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
      961 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1021 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1081 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1141 gagatccagt ttggttaatt aacaactttg tatagaaaag ttggctccga atttctcgag
     1201 gaattcggca ggaagagggc ctatttccca tgattccttc atatttgcat atacgataca
     1261 aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa
     1321 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     1381 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     1441 tcttgtggaa aggacgaaac accgaacccc taccaactgg tcggggtttg aaactgagac
     1501 catcggtctc atttttttaa gcttatagta atcaattacg gggtcattag ttcatagccc
     1561 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1621 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1681 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1741 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     1801 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     1861 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     1921 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     1981 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2041 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
     2101 gatccccggg taactgatca taattcgacc caagtttgta caaaaaagca ggctgattac
     2161 cggagaattc caattggcgg ccgcaccggt gccaccatgc caaagaagaa gcggaaggtc
     2221 ggtggcggct cacccgggat gtccaattta ctgaccgtac accaaaattt gcctgcatta
     2281 ccggtcgatg caacgagtga tgaggttcgc aagaacctga tggacatgtt cagggatcgc
     2341 caggcgtttt ctgagcatac ctggaaaatg cttctgtccg tttgccggtc gtgggcggca
     2401 tggtgcaagt tgaataaccg gaaatggttt cccgcagaac ctgaagatgt tcgcgattat
     2461 cttctatatc ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca tttgggccag
     2521 ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca
     2581 ctggttatgc ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct
     2641 ctagcgttcg aacgcactga tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc
     2701 tgccaggata tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata
     2761 gccgaaattg ccaggatcag ggttaaagat atctcacgta ctgacggtgg gagaatgtta
     2821 atccatattg gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa ggcacttagc
     2881 ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtagc tgatgatccg
     2941 aataactacc tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc
     3001 cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt gatttacggc
     3061 gctaaggatg actctggtca gagatacctg gcctggtctg gacacagtgc ccgtgtcgga
     3121 gccgcgcgag atatggcccg cgctggagtt tcaataccgg agatcatgca agctggtggc
     3181 tggaccaatg taaatattgt catgaactat atccgtaacc tggatagtga aacaggggca
     3241 atggtgcgcc tgctggaaga tggcgatact agtggtggcg gctcaaagcg tcctgctgct
     3301 actaagaaag ctggtcaagc taagaaaaag aaatctagat agtgactcga gactagataa
     3361 ctgatctacc cagctttctt gtacaaagtg gtgaattccg gcaattcgat atcaagctta
     3421 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3481 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3541 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     3601 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3661 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3721 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3781 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     3841 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3901 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3961 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     4021 accgtcgacc tcgagtccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     4081 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     4141 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     4201 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     4261 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     4321 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     4381 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     4441 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     4501 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtgtctctgg ttagaccaga
     4561 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     4621 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     4681 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga
     4741 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     4801 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     4861 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     4921 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     4981 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     5041 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5101 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5161 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5221 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5281 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5341 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5401 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     5461 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5521 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     5581 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     5641 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5701 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     5761 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     5821 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     5881 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     5941 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     6001 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     6061 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     6121 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     6181 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     6241 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     6301 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     6361 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     6421 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6481 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6541 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6601 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6661 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6721 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6781 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6841 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6901 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6961 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7021 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7081 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7141 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7201 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7261 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7321 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7381 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7441 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7501 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7561 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7621 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7681 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7741 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7801 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7861 cagtgctgca atgataccgc gtgacccacg ctcaccggct ccagatttat cagcaataaa
     7921 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7981 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8041 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8101 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8161 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8221 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8281 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8341 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8401 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8461 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8521 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8581 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8641 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8701 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     8761 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     8821 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     8881 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     8941 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     9001 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     9061 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     9121 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     9181 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     9241 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     9301 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     9361 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     9421 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     9481 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     9541 tatataagca gcgcgttttg cctgtactgg gtgtctctgg ttagaccaga tctgagcctg
     9601 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     9661 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     9721 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     9781 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     9841 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     9901 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     9961 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
    10021 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
    10081 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
    10141 tataatacag tagcaaccct ctattgtgtg cat
//
