LOCUS       V043939                12503 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043939
VERSION     V043939
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     polyA_signal    216..350
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     promoter        398..502
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             503..1363
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      1534..2119
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    2407..2428
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        2443..2473
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2481..2497
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     2505..2521
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     LTR             2650..3283
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    3490..4078
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     CDS             complement(4092..4691)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     CDS             complement(4701..4754)
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             complement(4824..5486)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     regulatory      5507..5516
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     promoter        complement(5540..5869)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             complement(5880..6626)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of
                     doxycycline"
                     /transl_table=1
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG*"
                     /label="Tet-On® 3G"
     promoter        complement(6645..7155)
                     /note="human phosphoglycerate kinase 1 promoter"
                     /label="hPGK promoter"
     promoter        7172..7539
                     /note="3rd-generation Tet-responsive promoter that can be
                     activated by binding of Tet-On® 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription
                     factors"
                     /label="TRE3GS promoter"
     CDS             7552..9933
                     /label="UFL1(NM_015323)"
                     /note="UFL1(NM_015323)"
                     /gene="UFL1"
     CDS             9940..10005
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    10183..10317
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     misc_feature    10361..10476
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     CDS             complement(10739..10783)
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    10968..11201
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     misc_feature    11698..11823
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     LTR             11870..12503
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
ORIGIN
        1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg
       61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg
      121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt
      181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg
      241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga
      361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct
     1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc
     2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg
     2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac
     2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag
     2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga
     3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca
     3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta
     3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct
     3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga
     3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt
     3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt
     3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg
     3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat
     3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa
     3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga
     3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga
     3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca
     3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag
     3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag
     3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca
     3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac
     3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag
     4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt
     4081 tccagacgcg ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc
     4141 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc
     4201 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc
     4261 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg
     4321 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg
     4381 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc
     4441 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac
     4501 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc
     4561 tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg
     4621 gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg
     4681 tactcggtca tggtcgcgtc agggccggga ttctcctcca cgtcaccgca tgttagaaga
     4741 cttcctctgc cctcgcgaga tccggtggag ccgggtccgg cggtgccgtc cacggcagaa
     4801 ttggacgact gagcgcggga tctggcgaag gcgatggggg tcttgaaggc gtgctggtac
     4861 tccacgatgc ccagctcggt gttgctgtgc agctcctcca cgcggcggaa ggcgaacatg
     4921 gggcccccgt tctgcaggat gctggggtgg atggcgctct tgaagtgcat gtggctgtcc
     4981 accacgaagc tgtagtagcc gccgtcgcgc aggctgaagg tgcgggcgaa gctgcccacc
     5041 agcacgttat cgcccatggg gtgcaggtgc tccacggtgg cgttgctgcg gatgatcttg
     5101 tcggtgaaga tcacgctgtc ctcggggaag ccggtgccca ccaccttgaa gtcgccgatc
     5161 acgcggccgg cctcgtagcg gtagctgaag ctcacgtgca gcacgccgcc gtcctcgtac
     5221 ttctcgatgc gggtgttggt gtagccgccg ttgttgatgg cgtgcaggaa ggggttctcg
     5281 tagccgctgg ggtaggtgcc gaagtggtag aagccgtagc ccatcacgtg gctcagcagg
     5341 taggggctga aggtcagggc gcctttggtg ctcttcatct tgttggtcat gcggccctgc
     5401 ttgggggtgc cctctccgcc gcccaccagc tcgaactcca cgccgttcag ggtgccggtg
     5461 atgcggcact cgatctccat ggcgggcagg ccgctctcgt cgctctccat ggtggcgtct
     5521 agcgtaggcg ccggtcacat ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     5581 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     5641 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     5701 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     5761 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     5821 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttcgcgcgtt
     5881 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg
     5941 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg
     6001 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg
     6061 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga
     6121 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc
     6181 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg
     6241 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac
     6301 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga
     6361 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg
     6421 gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc
     6481 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt
     6541 ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc agagtttatg
     6601 actttgctct tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg
     6661 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg
     6721 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc
     6781 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc
     6841 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg
     6901 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga
     6961 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg
     7021 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc
     7081 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg
     7141 cgcaacccca accccgtgga attatcacct cgagtttact ccctatcagt gatagagaac
     7201 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca
     7261 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt
     7321 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac
     7381 gtatatccag tttactccct atcagtgata gagaacgtat aagctttgct tatgtaaacc
     7441 agggcgccta taaaagagtg ctgatttttt gagtaaactt caattccaca acacttttgt
     7501 cttataccaa ctttccgtac cacttcctac cctcgtaaag aattcgccac catggcggac
     7561 gcctgggaag agattaggcg gttggcggcc gacttccagc gggcgcagtt cgccgaggcc
     7621 acgcagaggt tgtccgagcg gaactgcatt gagattgtta ataaattgat tgctcagaaa
     7681 cagctagaag tagttcatac actcgatgga aaggaatata ttactccagc ccaaattagt
     7741 aaagaaatga gagatgagct acatgtccga ggtggtcgag taaacattgt tgatctacaa
     7801 caggtaatta atgtggacct gattcatatt gaaaatagaa ttggtgacat tattaaatca
     7861 gaaaagcatg ttcagttagt gttgggacaa ctgatagatg agaattattt ggatcggttg
     7921 gcagaagagg tcaatgataa attgcaagaa agtggtcagg tcaccatatc agaactgtgt
     7981 aaaacttatg atcttcctgg gaactttctg acacaggcac taactcagcg acttggtaga
     8041 attatcagtg gacatattga tcttgataat agaggagtaa tttttacgga agcttttgta
     8101 gctcgacata aagcacgtat ccgtggacta ttcagtgcta ttacccggcc tacagctgtg
     8161 aattctttga tttcaaaata tggatttcag gagcagcttc tttactctgt gcttgaggaa
     8221 cttgttaata gcggacgctt acgaggcact gtggttggtg ggagacagga taaagctgtg
     8281 tttgtccctg acatctactc caggacacag agtacttggg tggattcctt tttcaggcag
     8341 aatggctatc tagaatttga tgctttgtcc agacttggaa tcccagatgc tgtaagctac
     8401 ataaagaaaa gatataagac tacacaactc ttgtttttga aagcagcttg tgttggtcaa
     8461 ggacttgtgg atcaagtgga agcatcagta gaagaagcca tcagctctgg aacatgggtt
     8521 gatattgcac ctctgctacc cacttcttta tcagttgaag atgctgccat attgcttcag
     8581 caggtgatga gggcattcag caaacaggcc tcaactgtag tctttagcga cactgttgta
     8641 gtcagtgaaa aatttataaa tgactgtaca gaactgttcc gtgagctgat gcaccagaaa
     8701 gctgaaaagg aaatgaaaaa taatcctgtg catttaatca ctgaagaaga tctgaaacaa
     8761 atctccactt tagaaagcgt tagtacaagt aaaaaggata aaaaagatga gcgaagaagg
     8821 aaagcaacag agggcagtgg aagcatgaga ggaggaggtg ggggcaatgc cagagagtac
     8881 aaaattaaaa aagtcaagaa gaaaggaaga aaagatgatg atagtgatga tgaatctcaa
     8941 tcatcccaca ctggaaagaa gaagccagag atcagtttta tgttccagga tgagattgaa
     9001 gattttttaa gaaaacacat acaagatgcc cctgaggagt ttatttcgga acttgctgag
     9061 tacttaataa aacctcttaa taaaacttat ctcgaggtgg tacgttcagt attcatgtct
     9121 tcaacaactt ctgcttctgg gacgggcaga aaacgcacaa tcaaggactt gcaagaagaa
     9181 gtttcaaacc tgtacaataa cattaggtta tttgaaaaag ggatgaagtt ttttgcagat
     9241 gacacacagg ctgctcttac caaacacttg ctgaagtcag tgtgtactga tatcactaac
     9301 ctcattttca acttcttagc ttcggattta atgatggcag tagacgatcc tgcagccatt
     9361 acaagtgaaa taagaaagaa aattttaagt aaattatcag aagaaaccaa agtagctctt
     9421 acaaaactcc ataactctct gaatgaaaag agcatagaag actttatttc ttgtctggat
     9481 tctgcagcag aagcttgtga tattatggtg aaaaggggag acaaaaaaag ggaaagacag
     9541 atactgttcc aacatcgaca agcactggct gaacagctaa aggtcacaga agaccctgct
     9601 cttattctgc acctcacatc agtcctgttg tttcagtttt caacccacag catgctccat
     9661 gcacctggaa gatgtgtccc acagatcatt gcttttctta atagtaaaat tccagaggat
     9721 cagcatgctc ttttggtaaa gtatcaaggt ttggttgtaa agcagctagt cagtcaaagt
     9781 aagaagactg ggcagggaga ttatcccttg aataatgaat tagacaaaga acaagaagat
     9841 gttgccagta ctactcgtaa agagcttcaa gaactttctt catccattaa agaccttgtt
     9901 ctcaaatcta ggaaatcatc tgtgacggaa gagggatccg actacaaaga ccatgacggt
     9961 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagag accacctccc
    10021 ctgcgagcta agctggacag ccaatgacgg gtaagagagt gacatttttc actaacctaa
    10081 gacaggaggg ccgtcagagc tactgcctaa tccaaagacg ggtaaaagtg ataaaaatgt
    10141 atcactccaa cctaagacag gcgcagcttc cgagggattt gagatccaga catgataaga
    10201 tacattgatg agtttggaca aaccaaaact agaatgcagt gaaaaaaatg ccttatttgt
    10261 gaaatttgtg atgctattgc cttatttgta accattataa gctgcaataa acaagttaag
    10321 tcgataaact ggatctctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat
    10381 ttgtttttgt agttctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc
    10441 tgcactgtac cccccaatcc ccccttttct tttaaaggcg ataccgtcga gatccgttca
    10501 ctaatcgaat ggatctgtct ctgtctctct ctccaccttc ttcttctatt ccttcgggcc
    10561 tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc
    10621 taactctatt cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta
    10681 ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca
    10741 aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg
    10801 attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca
    10861 tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag
    10921 gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat
    10981 cctttaggta tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg
    11041 tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca
    11101 ctataccaga caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag
    11161 tgcttcctgc tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt
    11221 ctctctgcac cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta
    11281 ctactttata tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc
    11341 tgaagatcag cggccggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc
    11401 tatcttgtct aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag
    11461 ggttgctact gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg
    11521 tagctgtccc agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc
    11581 gaatcgttct agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc
    11641 ccctggcctt aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac
    11701 gctctcgcac ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac
    11761 cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga
    11821 gagctcctct ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt
    11881 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
    11941 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
    12001 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca
    12061 aaaagcagct gcttatatgc aggatctgag ggctcgccac tccccagtcc cgcccaggcc
    12121 acgcctccct ggaaagtccc cagcggaaag tcccttgtag caagctcgat atcagcagtt
    12181 cttgaagtac tccggatgca gctctcgggc cacgtgatga aatgctaggc ggctgtcaaa
    12241 cctccactct aacacttctc tctccgggtc atccatccca tgcaggctca cagggtgtaa
    12301 caagctggtg ttctctcctt tattggcctc ttctacctta tctggctcaa ctggtactag
    12361 cttgtagcac catccaaagg tcagtggata tctgacccct ggccctggtg tgtagttctg
    12421 ctaatcaggg aagtagcctt gtgtgtggta gatccacaga tcaaggatat cttgtcttct
    12481 ttgggagtga attagccctt cca
//
