LOCUS       V040651                11393 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040651
VERSION     V040651
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     polyA_signal    216..350
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     promoter        398..502
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             503..1363
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      1534..2119
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    2407..2428
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        2443..2473
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2481..2497
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     2505..2521
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     LTR             2650..3283
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    3490..4078
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     CDS             complement(4092..4691)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     CDS             complement(4701..4754)
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             complement(4824..5486)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     regulatory      5507..5516
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     promoter        complement(5540..5869)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             complement(5880..6626)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of
                     doxycycline"
                     /transl_table=1
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG*"
                     /label="Tet-On® 3G"
     promoter        complement(6645..7155)
                     /note="human phosphoglycerate kinase 1 promoter"
                     /label="hPGK promoter"
     promoter        7172..7539
                     /note="3rd-generation Tet-responsive promoter that can be
                     activated by binding of Tet-On® 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription
                     factors"
                     /label="TRE3GS promoter"
     CDS             7552..8823
                     /label="STAMBP(NM_001353967)"
                     /note="STAMBP(NM_001353967)"
                     /gene="STAMBP"
     CDS             8830..8895
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    9073..9207
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     misc_feature    9251..9366
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     CDS             complement(9629..9673)
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    9858..10091
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     misc_feature    10588..10713
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     LTR             10760..11393
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
ORIGIN
        1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg
       61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg
      121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt
      181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg
      241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga
      361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct
     1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc
     2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg
     2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac
     2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag
     2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga
     3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca
     3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta
     3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct
     3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga
     3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt
     3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt
     3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg
     3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat
     3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa
     3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga
     3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga
     3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca
     3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag
     3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag
     3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca
     3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac
     3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag
     4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt
     4081 tccagacgcg ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc
     4141 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc
     4201 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc
     4261 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg
     4321 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg
     4381 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc
     4441 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac
     4501 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc
     4561 tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg
     4621 gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg
     4681 tactcggtca tggtcgcgtc agggccggga ttctcctcca cgtcaccgca tgttagaaga
     4741 cttcctctgc cctcgcgaga tccggtggag ccgggtccgg cggtgccgtc cacggcagaa
     4801 ttggacgact gagcgcggga tctggcgaag gcgatggggg tcttgaaggc gtgctggtac
     4861 tccacgatgc ccagctcggt gttgctgtgc agctcctcca cgcggcggaa ggcgaacatg
     4921 gggcccccgt tctgcaggat gctggggtgg atggcgctct tgaagtgcat gtggctgtcc
     4981 accacgaagc tgtagtagcc gccgtcgcgc aggctgaagg tgcgggcgaa gctgcccacc
     5041 agcacgttat cgcccatggg gtgcaggtgc tccacggtgg cgttgctgcg gatgatcttg
     5101 tcggtgaaga tcacgctgtc ctcggggaag ccggtgccca ccaccttgaa gtcgccgatc
     5161 acgcggccgg cctcgtagcg gtagctgaag ctcacgtgca gcacgccgcc gtcctcgtac
     5221 ttctcgatgc gggtgttggt gtagccgccg ttgttgatgg cgtgcaggaa ggggttctcg
     5281 tagccgctgg ggtaggtgcc gaagtggtag aagccgtagc ccatcacgtg gctcagcagg
     5341 taggggctga aggtcagggc gcctttggtg ctcttcatct tgttggtcat gcggccctgc
     5401 ttgggggtgc cctctccgcc gcccaccagc tcgaactcca cgccgttcag ggtgccggtg
     5461 atgcggcact cgatctccat ggcgggcagg ccgctctcgt cgctctccat ggtggcgtct
     5521 agcgtaggcg ccggtcacat ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     5581 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     5641 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     5701 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     5761 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     5821 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttcgcgcgtt
     5881 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg
     5941 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg
     6001 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg
     6061 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga
     6121 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc
     6181 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg
     6241 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac
     6301 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga
     6361 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg
     6421 gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc
     6481 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt
     6541 ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc agagtttatg
     6601 actttgctct tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg
     6661 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg
     6721 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc
     6781 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc
     6841 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg
     6901 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga
     6961 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg
     7021 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc
     7081 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg
     7141 cgcaacccca accccgtgga attatcacct cgagtttact ccctatcagt gatagagaac
     7201 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca
     7261 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt
     7321 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac
     7381 gtatatccag tttactccct atcagtgata gagaacgtat aagctttgct tatgtaaacc
     7441 agggcgccta taaaagagtg ctgatttttt gagtaaactt caattccaca acacttttgt
     7501 cttataccaa ctttccgtac cacttcctac cctcgtaaag aattcgccac catgtctgac
     7561 catggagatg tgagcctccc gcccgaagac cgggtgaggg ctctctccca gctgggtagt
     7621 gcggtagagg tgaatgaaga cattccaccc cgtcggtact tccgctctgg agttgagatt
     7681 atccgaatgg catccattta ctctgaggaa ggcaacattg aacatgcctt catcctctat
     7741 aacaagtata tcacgctctt tattgagaaa ctaccaaaac atcgagatta caaatctgct
     7801 gtcattcctg aaaagaaaga cacagtaaag aaattaaagg agattgcatt tcccaaagca
     7861 gaagagctga aggcagagct gttaaaacga tataccaaag aatatacaga atataatgaa
     7921 gaaaagaaga aggaagcaga ggaattggcc cggaacatgg ccatccagca agagctggaa
     7981 aaggaaaaac agagggtagc acaacagaag cagcagcaat tggaacagga acagttccat
     8041 gccttcgagg agatgatccg gaaccaggag ctagaaaaag agcgactgaa aattgtacag
     8101 gagtttggga aggtagaccc tggcctaggt ggcccgctag tgcctgactt ggagaagccc
     8161 tccttagatg tgttccccac cttaacagtc tcatccatac agccttcaga ctgtcacaca
     8221 actgtaaggc cagctaagcc acctgtggtg gacaggtcct tgaaacctgg agcactgagc
     8281 aactcagaaa gtattcccac aatcgatgga ttgcgccatg tggtggtgcc tgggcggctg
     8341 tgcccacagt ttctccagtt agccagtgcc aacactgccc ggggagtgga gacatgtgga
     8401 attctctgtg gaaaactgat gaggaatgaa tttaccatta cccatgttct catccccaag
     8461 caaagtgctg ggtctgatta ctgcaacaca gagaacgaag aagaactttt cctcatacag
     8521 gatcagcagg gcctcatcac actgggctgg attcatactc accccacaca gaccgcgttt
     8581 ctctccagtg tcgacctaca cactcactgc tcttaccaga tgatgttgcc agagtcagta
     8641 gccattgttt gctcccccaa gttccaggaa actggattct ttaaactaac tgaccatgga
     8701 ctagaggaga tttcttcctg tcgccagaaa ggatttcatc cacacagcaa ggatccacct
     8761 ctgttctgta gctgcagcca cgtgactgtt gtggacagag cagtgaccat cacagacctt
     8821 cgaggatccg actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
     8881 gatgacgatg acaagtagag accacctccc ctgcgagcta agctggacag ccaatgacgg
     8941 gtaagagagt gacatttttc actaacctaa gacaggaggg ccgtcagagc tactgcctaa
     9001 tccaaagacg ggtaaaagtg ataaaaatgt atcactccaa cctaagacag gcgcagcttc
     9061 cgagggattt gagatccaga catgataaga tacattgatg agtttggaca aaccaaaact
     9121 agaatgcagt gaaaaaaatg ccttatttgt gaaatttgtg atgctattgc cttatttgta
     9181 accattataa gctgcaataa acaagttaag tcgataaact ggatctctgc tgtccctgta
     9241 ataaacccga aaattttgaa tttttgtaat ttgtttttgt agttctttag tttgtatgtc
     9301 tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct
     9361 tttaaaggcg ataccgtcga gatccgttca ctaatcgaat ggatctgtct ctgtctctct
     9421 ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg
     9481 tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa
     9541 aaactattct taaacctacc aagcctccta ctatcattat gaataatttt atataccaca
     9601 gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt
     9661 cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg
     9721 tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca
     9781 gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag
     9841 agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc
     9901 ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa
     9961 tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta
    10021 ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga
    10081 acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg
    10141 tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc
    10201 aattgtccct catatctcct cctccaggtc tgaagatcag cggccggccg cttgctgtgc
    10261 ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt
    10321 tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag
    10381 ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc
    10441 tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc ttgcccatac
    10501 tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg
    10561 cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc
    10621 tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg
    10681 cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa
    10741 gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat
    10801 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
    10861 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
    10921 tggtctaacc agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag
    10981 ggctcgccac tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag
    11041 tcccttgtag caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc
    11101 cacgtgatga aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc
    11161 atccatccca tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc
    11221 ttctacctta tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata
    11281 tctgacccct ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta
    11341 gatccacaga tcaaggatat cttgtcttct ttgggagtga attagccctt cca
//
