LOCUS       V014837                10550 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014837
VERSION     V014837
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(216..350)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        398..502
                     /label="AmpR promoter"
     CDS             503..1360
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1534..2119
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2407..2428
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2443..2473
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     LTR             complement(2650..3283)
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     misc_feature    complement(3490..4078)
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(4095..5117)
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(5166..5495)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             complement(5509..6252)
                     /label="Tet-On(R) 3G"
                     /note="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of
                     doxycycline"
     promoter        complement(6271..6781)
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     promoter        6798..7165
                     /label="TRE3GS promoter"
                     /note="3rd-generation Tet-responsive promoter that can be
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription
                     factors"
     CDS             7195..7218
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             7219..8046
                     /gene="ptpB"
                     /label="Triple specificity protein phosphatase PtpB"
                     /note="Triple specificity protein phosphatase PtpB from
                     Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
                     Accession#: I6WXK4"
     polyA_signal    complement(8230..8364)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(8407..8524)
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             complement(8596..8637)
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     CDS             complement(8786..8830)
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     misc_feature    complement(9015..9248)
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     misc_feature    complement(9745..9870)
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     LTR             complement(9917..10550)
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg
       61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg
      121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt
      181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg
      241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga
      361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct
     1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc
     2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg
     2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac
     2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag
     2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga
     3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca
     3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta
     3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct
     3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga
     3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt
     3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt
     3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg
     3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat
     3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa
     3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga
     3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga
     3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca
     3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag
     3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag
     3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca
     3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac
     3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag
     4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt
     4081 tccagacgcg tctattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg
     4141 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta
     4201 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
     4261 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
     4321 atatacgccc ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc
     4381 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat
     4441 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc
     4501 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc
     4561 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc
     4621 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat
     4681 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga
     4741 tcggccgcag cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg
     4801 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg
     4861 ctctcgctga attccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag
     4921 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca
     4981 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc
     5041 atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg
     5101 agttcaggct ttttcatcac gtgctgatca gatccgaaaa tggatataca agctcccggg
     5161 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     5221 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     5281 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     5341 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     5401 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     5461 gagcctgggg actttccaca ccctaactga cacacattcg cgcgtttacc cggggagcat
     5521 gtcaaggtca aaatcgtcaa gagcgtcagc aggcagcata tcaaggtcaa agtcgtcaag
     5581 ggcatcggct gggagcatgt ctaagtcaaa atcgtcaagg gcgtcggtcg gcccgccgct
     5641 ttcgcacttt agctgtttct ccaggccaca tatgattagt tccaggccga aaaggaaggc
     5701 aggttcggct ccctgccggt cgaacagctc aattgcttgt ttcagaagtg ggggcataga
     5761 atcggtggta ggtgtctctc tttcctcttt tgctacttga tgctcctgtt cctccaatac
     5821 gcagcccagt gtaaagtggc ccacggcgga cagagcgtac agtgcgttct ccagggagaa
     5881 gccttgctga cacaggaacg cgagctgatt ttccagggtt tcgtactgtt tctctgttgg
     5941 gcgggtgccg agatgcactt tagccccgtc gcgatgtgag aggagagcac agcggtatga
     6001 cttggcgttg ttccgcagaa agtcttgcca tgactcgcct tccagggggc aggagtgggt
     6061 atgatgcctg tccagcatct cgattggcag ggcatcgagc agggcccgct tgttcttcac
     6121 gtgccagtac agggtaggct gctcaactcc cagcttttga gcgagtttcc ttgtcgtcag
     6181 gccttcgata ccgactccat tgagtaattc cagagcagag tttatgactt tgctcttgtc
     6241 cagtctagac atggtaattc gatgatcccc ctggggagag aggtcggtga ttcggtcaac
     6301 gagggagccg actgccgacg tgcgctccgg aggcttgcag aatgcggaac accgcgcggg
     6361 caggaacagg gcccacacta ccgccccaca ccccgcctcc cgcaccgccc cttcccggcc
     6421 gctgctctcg gcgcgccctg ctgagcagcc gctattggcc acagcccatc gcggtcggcg
     6481 cgctgccatt gctccctggc gctgtccgtc tgcgagggta ctagtgagac gtgcggcttc
     6541 cgtttgtcac gtccggcacg ccgcgaaccg caaggaacct tcccgactta ggggcggagc
     6601 aggaagcgtc gccggggggc ccacaagggt agcggcgaag atccgggtga cgctgcgaac
     6661 ggacgtgaag aatgtgcgag acccagggtc ggcgccgctg cgtttcccgg aaccacgccc
     6721 agagcagccg cgtccctgcg caaacccagg gctgccttgg aaaaggcgca accccaaccc
     6781 cgtggaatta tcacctcgag tttactccct atcagtgata gagaacgtat gaagagttta
     6841 ctccctatca gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt
     6901 ataaggagtt tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt
     6961 gatagagaac gtatctacag tttactccct atcagtgata gagaacgtat atccagttta
     7021 ctccctatca gtgatagaga acgtataagc tttgcttatg taaaccaggg cgcctataaa
     7081 agagtgctga ttttttgagt aaacttcaat tccacaacac ttttgtctta taccaacttt
     7141 ccgtaccact tcctaccctc gtaaagaatt cccgtataca ccggtgccac catggattac
     7201 aaggacgacg atgacaagat ggctgtccgt gaactgccgg gcgcgtggaa ctttcgtgac
     7261 gtcgccgaca ccgcaaccgc attgcggccg gggcggctgt tccggtccag cgagctgagc
     7321 cgcctcgacg acgccggccg ggcaacgctg cgccggctgg ggatcaccga cgttgccgac
     7381 ctgcggtcgt cccgggaggt tgcccgccgc ggtccaggac gggttccgga cggcatcgac
     7441 gtccacctgc tgccgttccc cgacctcgcc gatgatgacg ccgacgactc agcgccgcac
     7501 gaaaccgcat tcaagaggct gctaaccaat gacgggtcca acggcgagtc cggcgaatcc
     7561 agccagtcga taaatgacgc ggccacccgc tacatgaccg acgagtatcg ccaattccca
     7621 acgcgcaatg gagcacagcg cgcgctacat cgtgtcgtca cactgcttgc cgccggacgc
     7681 ccggtgctca cccactgctt cgcgggtaag gatcgcaccg gcttcgtggt cgcgctggtg
     7741 cttgaagcgg tcggcctgga ccgcgacgtc atcgtcgccg actacctgcg cagcaacgac
     7801 tccgtgccac aactgcgggc ccggatctcc gagatgatcc agcagcgttt cgacaccgaa
     7861 ctggcacccg aggtggtgac gttcaccaag gcccggctgt ccgacggggt cctgggtgtc
     7921 cgcgcggagt acctggccgc cgcacgccag accattgacg agacctacgg atcgctgggc
     7981 ggctacctgc gcgacgccgg tatcagccag gccacagtca accggatgcg cggggtgctg
     8041 ctcggatgag gatccagacc acctcccctg cgagctaagc tggacagcca atgacgggta
     8101 agagagtgac atttttcact aacctaagac aggagggccg tcagagctac tgcctaatcc
     8161 aaagacgggt aaaagtgata aaaatgtatc actccaacct aagacaggcg cagcttccga
     8221 gggatttgag atccagacat gataagatac attgatgagt ttggacaaac caaaactaga
     8281 atgcagtgaa aaaaatgcct tatttgtgaa atttgtgatg ctattgcctt atttgtaacc
     8341 attataagct gcaataaaca agttaagtcg ataaactgga tctctgctgt ccctgtaata
     8401 aacccgaaaa ttttgaattt ttgtaatttg tttttgtagt tctttagttt gtatgtctgt
     8461 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt
     8521 aaaggcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc
     8581 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
     8641 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
     8701 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
     8761 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt
     8821 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt
     8881 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag
     8941 atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc
     9001 aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg
     9061 cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag
     9121 ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg
     9181 tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca
     9241 aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg
     9301 gtgctactcc taatggttca atttttacta ctttatattt atataattca cttctccaat
     9361 tgtccctcat atctcctcct ccaggtctga agatcagcgg ccggccgctt gctgtgcggt
     9421 ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat
     9481 ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc
     9541 ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga
     9601 tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat
     9661 atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga
     9721 tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc
     9781 gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc
     9841 ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc
     9901 cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct gagggatctc
     9961 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
    10021 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
    10081 tctaaccaga gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc
    10141 tcgccactcc ccagtcccgc ccaggccacg cctccctgga aagtccccag cggaaagtcc
    10201 cttgtagcaa gctcgatatc agcagttctt gaagtactcc ggatgcagct ctcgggccac
    10261 gtgatgaaat gctaggcggc tgtcaaacct ccactctaac acttctctct ccgggtcatc
    10321 catcccatgc aggctcacag ggtgtaacaa gctggtgttc tctcctttat tggcctcttc
    10381 taccttatct ggctcaactg gtactagctt gtagcaccat ccaaaggtca gtggatatct
    10441 gacccctggc cctggtgtgt agttctgcta atcagggaag tagccttgtg tgtggtagat
    10501 ccacagatca aggatatctt gtcttctttg ggagtgaatt agcccttcca
//
