LOCUS       V014220                 9692 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014220
VERSION     V014220
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        complement(2528..2895)
                     /label="TRE3GS promoter"
                     /note="3rd-generation Tet-responsive promoter that can be
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription
                     factors"
     promoter        2912..3422
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             3441..4184
                     /label="Tet-On(R) 3G"
                     /note="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of
                     doxycycline"
     promoter        4198..4527
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4576..5598
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     misc_feature    5615..6203
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6410..7043
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7172..7188)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7196..7212)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7220..7250)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7265..7286)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7574..8159)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8333..9190)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9191..9295)
                     /label="AmpR promoter"
     polyA_signal    9343..9477
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat
     2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg
     2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg
     2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca
     2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat
     2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc cgccggcacc ggtgtatacg
     2521 ggaattcttt acgagggtag gaagtggtac ggaaagttgg tataagacaa aagtgttgtg
     2581 gaattgaagt ttactcaaaa aatcagcact cttttatagg cgccctggtt tacataagca
     2641 aagcttatac gttctctatc actgataggg agtaaactgg atatacgttc tctatcactg
     2701 atagggagta aactgtagat acgttctcta tcactgatag ggagtaaact ggtcatacgt
     2761 tctctatcac tgatagggag taaactcctt atacgttctc tatcactgat agggagtaaa
     2821 gtctgcatac gttctctatc actgataggg agtaaactct tcatacgttc tctatcactg
     2881 atagggagta aactcgaggt gataattcca cggggttggg gttgcgcctt ttccaaggca
     2941 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
     3001 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
     3061 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
     3121 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
     3181 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
     3241 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
     3301 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
     3361 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
     3421 agggggatca tcgaattacc atgtctagac tggacaagag caaagtcata aactctgctc
     3481 tggaattact caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc
     3541 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg
     3601 ccctgccaat cgagatgctg gacaggcatc atacccactc ctgccccctg gaaggcgagt
     3661 catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc
     3721 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg
     3781 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc
     3841 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag
     3901 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg aaacaagcaa
     3961 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca
     4021 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg
     4081 attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc
     4141 ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaaacg cgcgaatgtg
     4201 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4261 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4321 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4381 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4441 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4501 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     4561 tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     4621 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     4681 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     4741 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     4801 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
     4861 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     4921 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
     4981 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     5041 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     5101 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     5161 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     5221 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     5281 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     5341 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     5401 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     5461 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     5521 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     5581 cgtccgaggg caaaggaata gacgcgtctg gaacaatcaa cctctggatt acaaaatttg
     5641 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5701 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     5761 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     5821 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5881 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     5941 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     6001 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     6061 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6121 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     6181 ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat
     6241 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     6301 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     6361 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta
     6421 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     6481 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     6541 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     6601 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     6661 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     6721 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     6781 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     6841 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6901 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6961 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7021 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     7081 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt
     7141 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7201 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7261 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7321 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7381 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7441 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7501 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7561 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7621 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7681 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7741 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7801 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7861 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7921 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7981 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8041 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8101 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8161 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8221 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8281 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8341 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8401 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8461 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8521 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8581 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8641 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8701 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8761 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8821 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8881 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8941 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9001 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9061 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9121 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9181 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9241 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9301 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
     9361 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9421 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     9481 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     9541 gccaattacc tagtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     9601 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt
     9661 atttgttaaa tatgtactac aaacttagta gt
//
