LOCUS       Exported               10086 bp DNA     circular SYN 28-JUL-2025
DEFINITION  Exported.
ACCESSION   V015051
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10086)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10086)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(168..302)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        350..454
                     /label=AmpR promoter
     CDS             455..1312
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1486..2074
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2362..2383
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2398..2428
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     LTR             complement(2605..3238)
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     misc_feature    complement(3446..4034)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(4051..4842)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     promoter        complement(4859..5069)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             complement(5119..5865)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(5884..6394)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     promoter        6411..6778
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    6419..6437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6455..6473
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6491..6509
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6527..6545
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6563..6581
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6599..6617
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6635..6653
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      6784..6793
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6790..7506
                     /label=EGFP
                     /note="enhanced GFP"
     CDS             7525..7590
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7567..7590
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     polyA_signal    7766..7900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    7944..8059
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(8132..8173)
                     /label=Protein Tat
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     CDS             complement(8322..8366)
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     misc_feature    complement(8551..8784)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    complement(9281..9406)
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     LTR             complement(9453..10086)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaatga aaataattca
       61 gaggaatcac aggtttagag taaatgaaac cacaggtaat tggcagtggt aatagggtat
      121 ggggtgggaa gtttgggatg attttggtta gcttgagtta tccagttgat ccagacatga
      181 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      241 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      301 ttgatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      361 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      421 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      481 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
      541 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      601 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      661 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
      721 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
      781 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
      841 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
      901 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
      961 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1021 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1081 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1141 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1201 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1261 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     1321 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1381 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1441 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1501 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1561 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1621 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1681 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1741 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1801 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1861 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1921 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1981 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2041 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2101 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2161 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2221 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     2281 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     2341 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     2401 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     2461 aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacg gtaaaacgct
     2521 agcaatgtca aggcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa
     2581 taataagatg acatgaacta ctactgctag agattttcca cactgactaa aagggtctga
     2641 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     2701 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     2761 agatctggtc taaccagaga gacccagtac aggcaaaaag cagctgctta tatgcaggat
     2821 ctgagggctc gccactcccc agtcccgccc aggccacgcc tccctggaaa gtccccagcg
     2881 gaaagtccct tgtagcaagc tcgatatcag cagttcttga agtactccgg atgcagctct
     2941 cgggccacgt gatgaaatgc taggcggctg tcaaacctcc actctaacac ttctctctcc
     3001 gggtcatcca tcccatgcag gctcacaggg tgtaacaagc tggtgttctc tcctttattg
     3061 gcctcttcta ccttatctgg ctcaactggt actagcttgt agcaccatcc aaaggtcagt
     3121 ggatatctga cccctggccc tggtgtgtag ttctgctaat cagggaagta gccttgtgtg
     3181 tggtagatcc acagatcaag gatatcttgt cttcgttggg agtgaattag cccttccagt
     3241 cccctctttt cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt
     3301 aaaggtacct gaggtgtgac tggaaaaacc cacctcctcc tcctcttgtg cttctagcca
     3361 ggcacaatca gcattggtag ctgctgtatt gctacttgtg attgctccat gtttttctag
     3421 gtctcgactg cagaattaat tccaggcggg gaggcggccc aaagggagat ccgactcgtc
     3481 tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc gcgggaagga
     3541 aggtccgctg gattgagggc cgaagggacg tagcagaagg acgtcccgcg cagaatccag
     3601 gtggcaacac aggcgagcag ccatggaaag gacgtcagct tccccgacaa caccacggaa
     3661 ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg caaggcaggc ggcgatgagt
     3721 tccgccgtgg caatagggag ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg
     3781 gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag tgcacaccac gccacgttgc
     3841 ctgacaacgg gccacaactc ctcataaaga gacagcaacc aggatttata caaggaggag
     3901 aaaatgaaag ccatacggga agcaatagca tgatacaaag gcattaaagc agcgtatcca
     3961 catagcgtaa aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa
     4021 tccagaggtt gattgttcca gacgcgttca gaagaactcg tcaagaaggc gatagaaggc
     4081 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
     4141 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
     4201 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
     4261 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
     4321 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
     4381 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
     4441 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
     4501 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
     4561 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
     4621 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc
     4681 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
     4741 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
     4801 agcggccgga gaacctgcgt gcaatccatc ttgttcagcc atggtcgcgt tttgcaaaag
     4861 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
     4921 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     4981 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     5041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     5101 tgacacacat tcgcgcgttt acccggggag catgtcaagg tcaaaatcgt caagagcgtc
     5161 agcaggcagc atatcaaggt caaagtcgtc aagggcatcg gctgggagca tgtctaagtc
     5221 aaaatcgtca agggcgtcgg tcggcccgcc gctttcgcac tttagctgtt tctccaggcc
     5281 acatatgatt agttccaggc cgaaaaggaa ggcaggttcg gctccctgcc ggtcgaacag
     5341 ctcaattgct tgtttcagaa gtgggggcat agaatcggtg gtaggtgtct ctctttcctc
     5401 ttttgctact tgatgctcct gttcctccaa tacgcagccc agtgtaaagt ggcccacggc
     5461 ggacagagcg tacagtgcgt tctccaggga gaagccttgc tgacacagga acgcgagctg
     5521 attttccagg gtttcgtact gtttctctgt tgggcgggtg ccgagatgca ctttagcccc
     5581 gtcgcgatgt gagaggagag cacagcggta tgacttggcg ttgttccgca gaaagtcttg
     5641 ccatgactcg ccttccaggg ggcaggagtg ggtatgatgc ctgtccagca tctcgattgg
     5701 cagggcatcg agcagggccc gcttgttctt cacgtgccag tacagggtag gctgctcaac
     5761 tcccagcttt tgagcgagtt tccttgtcgt caggccttcg ataccgactc cattgagtaa
     5821 ttccagagca gagtttatga ctttgctctt gtccagtcta gacatggtaa ttcgatgatc
     5881 cccctgggga gagaggtcgg tgattcggtc aacgagggag ccgactgccg acgtgcgctc
     5941 cggaggcttg cagaatgcgg aacaccgcgc gggcaggaac agggcccaca ctaccgcccc
     6001 acaccccgcc tcccgcaccg ccccttcccg gccgctgctc tcggcgcgcc ctgctgagca
     6061 gccgctattg gccacagccc atcgcggtcg gcgcgctgcc attgctccct ggcgctgtcc
     6121 gtctgcgagg gtactagtga gacgtgcggc ttccgtttgt cacgtccggc acgccgcgaa
     6181 ccgcaaggaa ccttcccgac ttaggggcgg agcaggaagc gtcgccgggg ggcccacaag
     6241 ggtagcggcg aagatccggg tgacgctgcg aacggacgtg aagaatgtgc gagacccagg
     6301 gtcggcgccg ctgcgtttcc cggaaccacg cccagagcag ccgcgtccct gcgcaaaccc
     6361 agggctgcct tggaaaaggc gcaaccccaa ccccgtggaa ttatcacctc gagtttactc
     6421 cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag agaacgtatg
     6481 cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc tatcagtgat
     6541 agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta cagtttactc
     6601 cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag agaacgtata
     6661 agctttgctt atgtaaacca gggcgcctat aaaagagtgc tgattttttg agtaaacttc
     6721 aattccacaa cacttttgtc ttataccaac tttccgtacc acttcctacc ctcgtaaatc
     6781 agtgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     6841 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     6901 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     6961 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     7021 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     7081 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     7141 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     7201 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     7261 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     7321 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     7381 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     7441 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     7501 tacaaggaat tcaccggtgg atccgactac aaagaccatg acggtgatta taaagatcat
     7561 gacatcgact acaaggatga cgatgacaag tagaccacct cccctgcgag ctaagctgga
     7621 cagccaatga cgggtaagag agtgacattt ttcactaacc taagacagga gggccgtcag
     7681 agctactgcc taatccaaag acgggtaaaa gtgataaaaa tgtatcactc caacctaaga
     7741 caggcgcagc ttccgaggga tttgagatcc agacatgata agatacattg atgagtttgg
     7801 acaaaccaaa actagaatgc agtgaaaaaa atgccttatt tgtgaaattt gtgatgctat
     7861 tgccttattt gtaaccatta taagctgcaa taaacaagtt aagtcgataa actggatctc
     7921 tgctgtccct gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt
     7981 tagtttgtat gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa
     8041 tccccccttt tcttttaaag gcgataccgt cgagatccgt tcactaatcg aatggatctg
     8101 tctctgtctc tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg
     8161 gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata
     8221 gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat
     8281 tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct
     8341 aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag
     8401 tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc
     8461 caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa
     8521 atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc
     8581 acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg caacagatgc
     8641 tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc agacaataat
     8701 tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc tgctgctccc
     8761 aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg caccactctt
     8821 ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt atatttatat
     8881 aattcacttc tccaattgtc cctcatatct cctcctccag gtctgaagat cagcggccgg
     8941 ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt
     9001 ccttggtgtc ttttatctct atcctttgat gcacacaata gagggttgct actgtattat
     9061 ataatgatct aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt
     9121 gtctacagcc ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc
     9181 tgcttgccca tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa
     9241 ttttttccca tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc
     9301 tctccttcta gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc
     9361 ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc
     9421 ctttcgcttt caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa
     9481 gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag
     9541 cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc
     9601 ccaggctcag atctggtcta accagagaga cccagtacag gcaaaaagca gctgcttata
     9661 tgcaggatct gagggctcgc cactccccag tcccgcccag gccacgcctc cctggaaagt
     9721 ccccagcgga aagtcccttg tagcaagctc gatatcagca gttcttgaag tactccggat
     9781 gcagctctcg ggccacgtga tgaaatgcta ggcggctgtc aaacctccac tctaacactt
     9841 ctctctccgg gtcatccatc ccatgcaggc tcacagggtg taacaagctg gtgttctctc
     9901 ctttattggc ctcttctacc ttatctggct caactggtac tagcttgtag caccatccaa
     9961 aggtcagtgg atatctgacc cctggccctg gtgtgtagtt ctgctaatca gggaagtagc
    10021 cttgtgtgtg gtagatccac agatcaagga tatcttgtct tctttgggag tgaattagcc
    10081 cttcca
//
