LOCUS       V042276                11643 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042276
VERSION     V042276
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     promoter        2337..3519
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
                     /label="EF-1α promoter"
     regulatory      3542..3551
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             3551..3616
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             3632..6361
                     /label="MYRFL(NM_182530)"
                     /note="MYRFL(NM_182530)"
                     /gene="MYRFL"
     misc_feature    6393..6979
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             6999..7598
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7612..8200
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8407..9040
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9168..9184)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9192..9208
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9216..9246)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9261..9282
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9570..10158)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10329..11189)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11190..11294)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11342..11476
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtacgaatt
     3541 cgccaccatg gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     3601 ggatgacgat gacaagggtg gaggcggatc aatggatgtg gtaggcgaaa atgaggccct
     3661 gcagcagttc tttgaagctc agggagccaa tggtactctg gagaacccag ccctggacac
     3721 aagtctgttg gaggaatttc tgggcaatga ctttgatttg ggggccttgc aacgccagct
     3781 cccagacacc ccgccctatt ctgcatccga ctcatgctct cctccgcagg tcaaaggtgc
     3841 atgctaccca accctgaggc ccacagctgg gaggactcca gctccttttc tccaccccac
     3901 agctgctcct gcgatgccgc ccatgcaccc gctgcagagc acctctggaa tgggcgactc
     3961 ctgccaaatc cacggaggct ttcacagctg ccactcaaac gccagtcatc ttgccacccc
     4021 cctggaccaa tccgtgtcct cccatctggg gataggttgt tcttaccctc agcagcctct
     4081 gtgtcacagc cctggagcct cattgcctcc aaccaagaag agaaagtgca cgcaggcact
     4141 ggaggactcc ggggaatgcc gagtgtgggc ctgccactgc agaccgatga caagtaggag
     4201 tcgcagcagt gaagtccagg accctgacag tgagggacag aacagaatgc ctacagacca
     4261 gtgctctcct gctctgaagt ggcaaccatg ccatagtgtt ccttggcaca gcttattaaa
     4321 cagtcattat gaaaaactac ctgatgtggg ctatcgagtt gtaacagaca aaggatttaa
     4381 tttttcacca gcagatgaag cttttgtttg ccaaaagaag aatcatttcc agataaccat
     4441 tcacatccaa gtttggggaa gtccaaaatt tgttgaaacc gagatgggcc taaagccaat
     4501 agaaatgttt tacttgaaag tttttggcac taaggtggaa gctaccaatc aaataattgc
     4561 tattgaacag tcccaagcag ataggagcaa aaagattttc aatcctgtta aaattgacct
     4621 actggctgac caggtcacca aagtaacact gggacgatta cacttcagcg aaaccacagc
     4681 aaataatatg agaaaaaagg gaaaaccaaa tccagaccag agatacttca tgttggtggt
     4741 tggactgtat gctgctaacc aagaccagtt ctatctgttg tctgcccaca tctctgaaag
     4801 gatcattgta agggcctcta accctgggca gtttgaaaat gacagtgatg cattgtggca
     4861 gcgaggacaa gttccagaat ctattgtctg tcacggtcga gtaggaatca acacagatgc
     4921 gccggacgaa gccctggttg tctgtggcaa catgaaagtg atggggacca tcatgcatcc
     4981 ctctgacagc cgggcaaagc agaatatcca ggaggttgac acgaatgaac agctgaaaag
     5041 aatagcccaa atgagaattg ttgaatatga ctacaaacct gaatttgcat ctgcaatggg
     5101 aataaacact gcccatcaaa caggaatgat tgcccaggag gtgcaagaaa tcctgcccag
     5161 agcagtaaga gaggttggtg atgtcacctg cggaaacgga gagaccttgg agaacttcct
     5221 catggtggat aaggaccaga tctttatgga aaatgtaggt gcagtgaagc aactgtgcaa
     5281 actaactaac aaccttgagg aaagaataga agagttagaa atatggaaca gaaagctggc
     5341 ccggctaaag cggctcagta gttggaagtc atcagccagt gaagcaagca caatcagcaa
     5401 atctagcaga gccgttagtg catcttctcc aagaagggcc gttcataaaa aaaacaacaa
     5461 ggtttatttt tcaggaaaaa gacaggcgtg tcctaattgg gttttccaga ccttggttat
     5521 aactctgatt gctgtgatgg cattttgtgc tttgacgata gttgccctct atatacttag
     5581 cttaaaagat caagaccgac gtgtcccaaa tctccctcca agcaatatca caagctcaca
     5641 ggagccagct ctgctgccca cagcctcctc ctcagcacct aatacatccc tggtaaccac
     5701 accggcctcc ttacaagtac ctgaaattac tttctgtgaa atcctgccgt gtcaggagac
     5761 ttattgctgc cccatccggg gaatgaaaga agtctcttca agtcctgtgc aaagacaatc
     5821 tgaggagaag gaattccatc agaggcgatg gtcagaagac aaaagcaaat cagttttggc
     5881 aagaaatgca ctcagcggcc ctgactggga gagtgactgg attgatacaa ccatcagttc
     5941 tattcagatt atggaaatcc agcaaataat agatcatcag tattgcattc aaagcctcca
     6001 gtgcggatct ggaaattata attacaatat tcctgttaat aaacacacac ccaccaatgt
     6061 caagttctct ctggaaataa acacaacaga gccattgata gtcttccagt gcaaattcac
     6121 ccttggaaat atatgtttcc atagtaaaag gggaaccaaa gggctggaaa gccacagaga
     6181 aatctcccag gagatgacac agggatatca gcacatttgg agcctccctg tagccccatt
     6241 ttctgacagc atgttccatt tccgtgtagc tgcaccggat ttagctgact gttcaactga
     6301 tccttatttt gctgggatat tcttcaccga ttacttcttt tacttctatc gacgctgtgc
     6361 ctaaactagt tctagagcgg ccgcggatcc cgcccctctc cctccccccc ccctaacgtt
     6421 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     6481 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     6541 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     6601 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     6661 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     6721 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     6781 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     6841 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     6901 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     6961 tgataagctt gccacaaccc acaaggagac gaccttccat gaccgagtac aagcccacgg
     7021 tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg
     7081 ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg
     7141 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     7201 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     7261 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     7321 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     7381 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     7441 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     7501 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     7561 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctagac gcgtctggaa caatcaacct
     7621 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     7681 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     7741 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     7801 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     7861 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7921 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7981 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     8041 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     8101 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     8161 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc
     8221 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     8281 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     8341 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga
     8401 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     8461 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
     8521 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
     8581 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
     8641 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
     8701 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
     8761 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
     8821 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
     8881 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8941 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     9001 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     9061 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat
     9121 tgctagcgtt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     9181 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     9241 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     9301 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9361 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9421 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9481 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9541 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9601 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9661 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9721 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9781 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9841 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9901 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9961 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    10021 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    10081 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    10141 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10201 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10261 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10321 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10381 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10441 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10501 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10561 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10621 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10681 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10741 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10801 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10861 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10921 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10981 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    11041 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    11101 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11161 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11221 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11281 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
    11341 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    11401 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    11461 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    11521 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
    11581 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
    11641 agt
//
