LOCUS V024896 11525 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024896 VERSION V024896 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" promoter 2337..3519 /note="strong constitutive promoter for human elongation factor EF-1α" /label="EF-1α promoter" CDS 3551..3568 /codon_start=1 /product="6xHis affinity tag" /transl_table=1 /translation="HHHHHH" /label="6xHis" CDS 3569..6184 /label="GRM2(NM_000839)" /note="GRM2(NM_000839)" /gene="GRM2" CDS 6191..6256 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 6275..6861 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES2" CDS 6881..7480 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7494..8082 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 8289..8922 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(9050..9066) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 9074..9090 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(9098..9128) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 9143..9164 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9452..10040) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(10211..11071) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(11072..11176) /gene="bla" /label="AmpR promoter" polyA_signal 11224..11358 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtacgaatt 3541 cgccaccatg caccatcacc atcaccatat gggatcgctg cttgcgctcc tggcactgct 3601 gctgctgtgg ggtgctgtgg ctgagggccc agccaagaag gtgctgaccc tggagggaga 3661 cttggtgctg ggtgggctgt tcccagtgca ccagaagggc ggcccagcag aggactgtgg 3721 tcctgtcaat gagcaccgtg gcatccagcg cctggaggcc atgctttttg cactggaccg 3781 catcaaccgt gacccgcacc tgctgcctgg cgtgcgcctg ggtgcacaca tcctcgacag 3841 ttgctccaag gacacacatg cgctggagca ggcactggac tttgtgcgtg cctcactcag 3901 ccgtggtgct gatggctcac gccacatctg ccccgacggc tcttatgcga cccatggtga 3961 tgctcccact gccatcactg gtgttattgg cggttcctac agtgatgtct ccatccaggt 4021 ggccaacctc ttgaggctat ttcagatccc acagattagc tacgcctcta ccagtgccaa 4081 gctgagtgac aagtcccgct atgactactt tgcccgcaca gtgcctcctg acttcttcca 4141 agccaaggcc atggctgaga ttctccgctt cttcaactgg acctatgtgt ccactgtggc 4201 gtctgagggc gactatggcg agacaggcat tgaggccttt gagctagagg ctcgtgcccg 4261 caacatctgt gtggccacct cggagaaagt gggccgtgcc atgagccgcg cggcctttga 4321 gggtgtggtg cgagccctgc tgcagaagcc cagtgcccgc gtggctgtcc tgttcacccg 4381 ttctgaggat gcccgggagc tgcttgctgc cagccagcgc ctcaatgcca gcttcacctg 4441 ggtggccagt gatggttggg gggccctgga gagtgtggtg gcaggcagtg agggggctgc 4501 tgagggtgct atcaccatcg agctggcctc ctaccccatc agtgactttg cctcctactt 4561 ccagagcctg gacccttgga acaacagccg gaacccctgg ttccgtgaat tctgggagca 4621 gaggttccgc tgcagcttcc ggcagcgaga ctgcgcagcc cactctctcc gggctgtgcc 4681 ctttgagcag gagtccaaga tcatgtttgt ggtcaatgca gtgtacgcca tggcccatgc 4741 gctccacaac atgcaccgtg ccctctgccc caacaccacc cggctctgtg acgcgatgcg 4801 gccagttaac gggcgccgcc tctacaagga ctttgtgctc aacgtcaagt ttgatgcccc 4861 ctttcgccca gctgacaccc acaatgaggt ccgctttgac cgctttggtg atggtattgg 4921 ccgctacaac atcttcacct atctgcgtgc aggcagtggg cgctatcgct accagaaggt 4981 gggctactgg gcagaaggct tgactctgga caccagcctc atcccatggg cctcaccctc 5041 agccggcccc ctgcccgcct ctcgctgcag tgagccctgc ctccagaatg aggtgaagag 5101 tgtgcagccg ggcgaagtct gctgctggct ctgcattccg tgccagccct atgagtaccg 5161 attggacgaa ttcacttgcg ctgattgtgg cctgggctac tggcccaatg ccagcctgac 5221 tggctgcttc gaactgcccc aggagtacat ccgctggggc gatgcctggg ctgtgggacc 5281 tgtcaccatc gcctgcctcg gtgccctggc caccctcttt gtgctgggtg tctttgtgcg 5341 gcacaatgcc acaccagtgg tcaaggcctc aggtcgggag ctctgctaca tcctgctggg 5401 tggtgtcttc ctctgctact gcatgacctt catcttcatt gccaagccat ccacggcagt 5461 gtgtacctta cggcgtcttg gtttgggcac tgccttctct gtctgctact cagccctgct 5521 caccaagacc aaccgcattg cacgcatctt cggtggggcc cgggagggtg cccagcggcc 5581 acgcttcatc agtcctgcct cacaggtggc catctgcctg gcacttatct cgggccagct 5641 gctcatcgtg gtcgcctggc tggtggtgga ggcaccgggc acaggcaagg agacagcccc 5701 cgaacggcgg gaggtggtga cactgcgctg caaccaccgc gatgcaagta tgttgggctc 5761 gctggcctac aatgtgctcc tcatcgcgct ctgcacgctt tatgccttca agactcgcaa 5821 gtgccccgaa aacttcaacg aggccaagtt cattggcttc accatgtaca ccacctgcat 5881 catctggctg gcattcctgc ccatcttcta tgtcacctcc agtgactacc gggtacagac 5941 caccaccatg tgcgtgtcag tcagcctcag cggctccgtg gtgcttggct gcctctttgc 6001 gcccaagctg cacatcatcc tcttccagcc gcagaagaac gtggttagcc accgggcacc 6061 caccagccgc tttggcagtg ctgctgccag ggccagctcc agccttggcc aagggtctgg 6121 ctcccagttt gtccccactg tttgcaatgg ccgtgaggtg gtggactcga caacgtcatc 6181 gctttctaga gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa 6241 ggatgacgat gacaagtagc ggccgcggat cccgcccctc tccctccccc ccccctaacg 6301 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca 6361 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga 6421 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga 6481 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca 6541 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag 6601 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa 6661 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac 6721 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga 6781 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac 6841 gatgataagc ttgccacaac ccacaaggag acgaccttcc atgaccgagt acaagcccac 6901 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt 6961 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac 7021 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 7081 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 7141 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 7201 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 7261 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 7321 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 7381 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 7441 accgcgcacc tggtgcatga cccgcaagcc cggtgcctag acgcgtctgg aacaatcaac 7501 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 7561 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 7621 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 7681 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 7741 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 7801 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 7861 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg 7921 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 7981 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 8041 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag 8101 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga 8161 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt 8221 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 8281 gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat 8341 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag 8401 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga 8461 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga 8521 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt 8581 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg 8641 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc 8701 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc 8761 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 8821 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 8881 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 8941 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac 9001 attgctagcg tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 9061 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 9121 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 9181 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 9241 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 9301 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 9361 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 9421 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 9481 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 9541 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 9601 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 9661 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 9721 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 9781 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 9841 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 9901 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 9961 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 10021 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 10081 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 10141 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 10201 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 10261 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 10321 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 10381 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 10441 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 10501 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 10561 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 10621 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 10681 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 10741 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 10801 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 10861 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 10921 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 10981 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 11041 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 11101 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 11161 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag 11221 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 11281 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 11341 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc 11401 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat 11461 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta 11521 gtagt //