LOCUS       V042152                12153 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042152
VERSION     V042152
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     promoter        2337..3519
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
                     /label="EF-1α promoter"
     CDS             3548..6871
                     /label="Adnp(NM_001310086)"
                     /note="Adnp(NM_001310086)"
                     /gene="Adnp"
     misc_feature    6903..7489
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             7509..8108
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8122..8710
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8917..9550
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9678..9694)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9702..9718
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9726..9756)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9771..9792
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10080..10668)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10839..11699)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11700..11804)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11852..11986
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtacgaatt
     3541 cgccaccatg ttccaacttc ctgtcaacaa tcttggcagt ttaagaaaag cccggaaaac
     3601 tgtgaaaaaa atacttagtg acattgggtt ggaatactgt aaagaacata tagaagattt
     3661 taaacagttt gaacctaatg acttttattt gaaaaacact acatgggagg atgtaggact
     3721 gtgggaccct tctcttacga aaaatcagga ctatcggaca aaaccttttt gctgcagtgc
     3781 ttgtccgttt tcctcaaaat tcttctctgc ctacaaaagt catttccgga atgtccatag
     3841 tgaagacttt gaaaatagga ttctccttaa ctgcccttac tgtaccttca atgcagataa
     3901 aaagactttg gaaacacaca ttaaaatatt tcatgctcca aactccagcg caccaagtag
     3961 cagcctcagc actttcaaag ataaaaacaa aaacgatggc cttaaaccta agcaggctga
     4021 caatgtagag caagccgtgt attactgcaa gaagtgcact taccgagacc ctctctacga
     4081 gatcgtcagg aagcacatct acagggaaca ttttcaacac gtggcagcac cctacatagc
     4141 aaaagcagga gaaaaatcac tcaatggtgc agtctccctg ggcacaaatg cccgagagga
     4201 gtgtaacatc cactgcaagc gatgcctttt catgcccaag tcctatgaag ctttggtaca
     4261 gcatgtcatt gaggaccatg aacggatagg ctatcaggtc actgccatga tcggacacac
     4321 aaatgttgta gttccccgag ccaagccctt gatgctgata gctcccaaac ctcaagacaa
     4381 aaagggcatg ggactcccac cacgaatcag ctcccttgct tctggaaatg tccggtcgtt
     4441 gccatcacag cagatggtaa accgattgtc aataccaaag cccaacttaa attcaacggg
     4501 agtcaacatg atgtccaatg ttcacctgca gcaaaacaac tatggagtca aatctgtggg
     4561 ccagagctat ggtgttggcc agtcagtgag gctgggacta ggtggcaatg ctccagtttc
     4621 catccctcaa cagtctcagt ccgtgaaaca gttacttcca agtgggaatg ggaggtcttt
     4681 tgggctaggt gctgagcaga ggcccccagc agcagccagg tactccctgc agactgccaa
     4741 cacctctcta cccccaggcc aagtgaagtc tccctctgtg tctcagtcac aggcatctag
     4801 agtattaggt cagtccagtt ctaaacctcc accagccgcc acaggccctc ctccaagcaa
     4861 ccactgtgcc actcagaagt ggaaaatctg tacaatctgt aacgagcttt tccctgagaa
     4921 tgtctatagc gttcacttcg aaaaggagca taaagctgag aaagtcccag ccgtagctaa
     4981 ctacattatg aaaatacaca attttactag caaatgcctc tactgtaatc gctatttgcc
     5041 tacagatacc ctactcaacc atatgttaat tcatggtctg tcttgtccgt attgccgttc
     5101 caccttcaat gatgtagaga agatggcagc acacatgcga atggttcata ttgatgaaga
     5161 gatggggcct aaaacggatt ctactttgag ctttgatttg acattgcaac agggcagtca
     5221 caccaacatt catctcctgg tgaccacata caacctgagg gatgccccgg ctgaatcagt
     5281 tgcttaccat gcccaaaata atgccccagt tcctccaaag ccacaaccaa aagttcagga
     5341 aaaagcagat gtcccggtta aaagttcacc tcaagctgca gtgccctata aaaaagatgt
     5401 tgggaagacc ctttgccctc tttgcttttc aatactaaaa ggacccatat ctgatgcact
     5461 tgcacatcat ttacgagaaa gacaccaagt tattcagaca gttcatccgg ttgagaaaaa
     5521 gctaacttac aaatgtatcc attgccttgg tgtgtatact agcaacatga cagcctcaac
     5581 catcactctg catctagtcc actgcagggg tgttggaaaa acccagaatg gccaggacaa
     5641 gacaaacgca ccttctcggc tcaatcagtc tccaggcctg gcccctgtga agcgcacgta
     5701 tgagcagatg gagtttccac tgctaaaaaa gcggaagctg gaggaggatg ctgattcccc
     5761 tagctgcttt gaagagaagc cagaagagcc tgttgtttta gctttagacc ccaagggtca
     5821 tgaagatgat tcttatgagg ctaggaaaag ctttctcaca aagtacttca acaaacagcc
     5881 ctatcccacc aggagagaaa ttgagaagtt agctgccagt ctatggctat ggaagagtga
     5941 cattgcctcc catttcagta acaagaggaa gaagtgtgtc cgcgactgtg aaaagtacaa
     6001 gcctggtgtg ctgctaggtt ttaacatgaa agaattaaat aaagtcaaac acgagatgga
     6061 ttttgatgct gagtggctgt ttgaaaatca cgatgagaaa gactcaagag tcaatgctag
     6121 caagactgtt gacaaaaagc ataaccttgg gaaagaagat gatagcttct cagatagttt
     6181 tgaacatttg gaagaagaat ccaatggaag cgggagtcct tttgaccctg tctttgaagt
     6241 tgagcctaaa attcccagtg ataatttaga ggagcctgta ccgaaggtta ttccggaagg
     6301 tgctttggaa tctgagaagc tagaccaaaa agaggaggag gaggaggagg aggaggagga
     6361 tggttcaaaa tatgaaacta tccatttgac tgaggaacca gccaaattaa tgcatgatgc
     6421 ctctgatagt gaggtagacc aagatgatgt agttgagtgg aaagatggtg cttcaccatc
     6481 tgagagtggg cctggttccc aacaaatctc agactttgag gataatacat gtgaaatgaa
     6541 accaggaacc tggtctgatg agtcttccca gagtgaagat gcaaggagca gtaagccagc
     6601 tgccaaaaaa aaggctacag tgcaagatga cacagagcag ttaaaatgga agaatagttc
     6661 ctatggaaaa gttgaagggt tttggtccaa ggaccagtca cagtgggaaa atgcatctga
     6721 gaatgcagag cgcttaccaa acccacagat tgagtggcag aatagcacaa ttgacagtga
     6781 ggacggggag cagtttgaca gcatgactga cggagttgct gatcccatgc atggcagctt
     6841 aactggagtg aagctgagca gccagcaagc ctgaactagt tctagagcgg ccgcggatcc
     6901 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     6961 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     7021 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     7081 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     7141 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     7201 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     7261 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     7321 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     7381 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     7441 ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc acaaggagac
     7501 gaccttccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
     7561 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     7621 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     7681 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     7741 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     7801 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     7861 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     7921 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     7981 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     8041 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     8101 gtgcctagac gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
     8161 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     8221 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     8281 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     8341 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     8401 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8461 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     8521 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     8581 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8641 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8701 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa
     8761 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     8821 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     8881 tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac
     8941 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     9001 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     9061 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     9121 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     9181 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     9241 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     9301 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     9361 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     9421 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     9481 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     9541 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     9601 tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac ctctagctag
     9661 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9721 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     9781 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     9841 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9901 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9961 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    10021 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    10081 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    10141 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    10201 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    10261 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    10321 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    10381 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    10441 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    10501 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    10561 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    10621 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    10681 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    10741 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    10801 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    10861 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    10921 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    10981 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    11041 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    11101 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    11161 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    11221 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    11281 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    11341 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    11401 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    11461 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    11521 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    11581 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    11641 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    11701 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    11761 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    11821 gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta
    11881 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    11941 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
    12001 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
    12061 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
    12121 tatttgttaa atatgtacta caaacttagt agt
//
