LOCUS       V014759                10697 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014759
VERSION     V014759
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2338..3519
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             5354..5419
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     misc_feature    5447..6033
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             6053..6649
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6666..7254
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7461..8094
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8270..8300)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8315..8336)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8624..9212)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9386..10243)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10244..10348)
                     /label="AmpR promoter"
     polyA_signal    10396..10530
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtacgaatt
     3541 cgccaccatg acccctggaa ctcagagccc cttcttcctg ctgctgatcc tgaccgtgct
     3601 gaccgtcgtg acaggcagcg gccacacaaa ttctacacct ggcggcgaga aagagacaag
     3661 cgccacacag agaagcagca tgcccatcag caccaagaac gccgtgtcca tgagcagcag
     3721 actgtccagc cactctcctg tgtctggcag cagcacaacc cagggccaag atgtgacact
     3781 ggccctggct atggaacctg ccacaggctc tgctacaaca ctgggccaca atgtgactag
     3841 cgcccctgat acaagcgccg ctccaggatc tacaggacct cctgccagag tggtcacaag
     3901 cgccccagac acatctgctg cccctggttc tactggacca ccagccaggg ttgtgacatc
     3961 tgccccagat accagtgccg cacctggaag tacaggccct ccagctagag tcgtgacctc
     4021 cgctcctgat acttctgccg ctcctggtag caccggtcca ccagcaagag tggttaccag
     4081 cgctcccgat acctcagctg cacctggcag cactggaccg cctgctagag ttgttacctc
     4141 tgcacccgac acaagcgcag ccccaggcag taccggtcct cctgcacgtg tcgttacaag
     4201 tgcccctgac actagtgcag ctcccggaag cactgggcct ccagcaaggg tcgtcactag
     4261 tgctcccgac acttctgcag caccaggctc aaccgggcct cctgcaagag ttgtgacttc
     4321 agctccagac acctccgctg ctccagggtc aacagggcca ccagctcgtg tggttacttc
     4381 agcccctggc acatcagctg ctcccggctc tacagctcca cctggatcta ctgctcctcc
     4441 tgctcacgat gtgacctctg ccagcgattc tgcctctggc tctgcctcta cactggtgca
     4501 cagcaccaca tctgccagag ccacaacaac ccctgcctcc aagagcaccc ctttcagcat
     4561 tcctagccac cacagcgaca cccctaccac actggcctct cactccacca agacagatgc
     4621 cagcagcacc caccactcta ccgtgccacc tttcaccagc tccaaccaca gcacaagccc
     4681 acagctgagc ctgggcgtct cattcttctt tctgtccttc cacatcagca acctgcagtt
     4741 caacagcagc ctggaagatc ccagcaccaa ctactaccag cagctgcagc gggatatcag
     4801 cgagctgttc ctgcagatct acaagcaggg cgacttcctg ggcctgagca acatcatgtt
     4861 cagacccggc tctgtggtgg tgcagagcac actggtgttc agagaaggca ccaccaacgt
     4921 gcacgacgtg gaaacccagt tcaaccagag aaagaccgag gccgccagcc ggtacaatct
     4981 gaccatcagc gatatctccg tgcgggacgt gccctttcca ttctctgctc aaacaggcgc
     5041 tggcgtgcca ggatggggaa ttgctctgct ggtgctcgtg tgtgtgctgg tggtgctggc
     5101 catcgtgtac tttattgccc tggccgtgtg ccagtgccgg cagaagaatt acagacagct
     5161 ggacatcttc cccgctcggg acgcctacca tcctatgagc gagtacccca cctatcacac
     5221 ccacggcaga tatgttcccg caggcggcac caatagaagc ccctacgaag aagtgtccgc
     5281 cggcaacggt ggaagcagcc tgagctatac aaatcctgcc gtggccgcca ccagcgccaa
     5341 tctgcgcggc cgcgactaca aagaccatga cggtgattat aaagatcatg acatcgacta
     5401 caaggatgac gatgacaagt aggcctgcga tatccagcgg atcccgcccc tctccctccc
     5461 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     5521 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     5581 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     5641 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     5701 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     5761 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     5821 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     5881 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     5941 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     6001 tttgaaaaac acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga
     6061 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     6121 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     6181 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6241 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6301 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6361 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6421 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6481 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6541 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6601 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct agacgcgtct
     6661 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6721 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6781 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6841 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6901 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6961 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7021 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     7081 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7141 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7201 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     7261 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     7321 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     7381 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7441 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct
     7501 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     7561 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     7621 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     7681 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     7741 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     7801 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     7861 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     7921 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7981 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8041 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     8101 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8161 agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat
     8221 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8281 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8341 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8401 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8461 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8521 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8581 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8641 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8701 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8761 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8821 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8881 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8941 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9001 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9061 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9121 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9181 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9241 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9301 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9361 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9421 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9481 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9541 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9601 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9661 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9721 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9781 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9841 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9901 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9961 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10021 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10081 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10141 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10201 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10261 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10321 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    10381 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    10441 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10501 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    10561 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    10621 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    10681 actacaaact tagtagt
//
