LOCUS       V014810                10502 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014810
VERSION     V014810
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2338..3519
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3548..5149
                     /gene="CD276"
                     /label="CD276 antigen"
                     /note="CD276 antigen from Homo sapiens. Accession#: Q5ZPR3"
     CDS             5159..5224
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     misc_feature    5252..5838
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5858..6454
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6471..7059
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7266..7899
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8075..8105)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8120..8141)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8429..9017)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9191..10048)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10049..10153)
                     /label="AmpR promoter"
     polyA_signal    10201..10335
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtacgaatt
     3541 cgccaccatg ctgcatagaa gaggcagccc tggcatggga gtgcatgttg gagctgctct
     3601 tggagccctg tggttctgtt tgacaggcgc cctggaagtg caggtcccag aggatcctgt
     3661 ggtggccctt gtgggaacag acgccacact gagatgcagc ttcagccctg agcctggctt
     3721 tagtctggcc cagctgaacc tgatctggca gctgaccgat accaagcagc tggtgcacag
     3781 cttcaccgag ggcagagatc agggaagcgc ctacgccaat agaacagccc tgttcctgga
     3841 tctgctggcc cagggaaatg ccagcctgag actgcagaga gtcagagttg ccgacgaggg
     3901 cagctttacc tgcttcgtgt ccatcagaga cttcggctct gccgccgtgt ctctgcaagt
     3961 ggccgctcct tacagcaagc ccagcatgac cctggaacct aacaaggacc tgaggcctgg
     4021 cgacaccgtg accatcacat gcagcagcta cagaggctac cctgaggccg aagtgttctg
     4081 gcaggatgga caaggcgctc ctctgaccgg caatgtgacc acaagccaga tggccaatga
     4141 gcagggcctg ttcgatgtgc acagcgtgct gagagtggtg ctgggcgcca atggcacata
     4201 ctcttgcctc gtgcggaacc ccgttctgca gcaagatgcc cacggcagca tcaccatcac
     4261 accccagaga agtcctactg gcgccgtcga agtgcaagtg cctgaagatc cagtggtcgc
     4321 tctcgtgggc accgatgcaa ccctgagatg ctcttttagc cccgagccag gattcagcct
     4381 ggctcagctc aatctcattt ggcagctcac agacacaaaa cagctggtcc actcctttac
     4441 agaggggcgc gatcagggat ctgcctatgc caacaggacc gctctgtttc tggacctcct
     4501 cgctcagggc aacgcctctc tgagactcca aagagtgcgg gtcgccgacg aaggctcctt
     4561 cacatgcttc gtcagcatcc gcgatttcgg cagtgctgct gtcagcctgc aagtcgctgc
     4621 cccttactct aagccctcca tgacactcga gcccaacaag gatctcagac ccggcgatac
     4681 agtgaccatt acctgctcta gctaccgggg ctatcccgaa gctgaggttt tctggcaaga
     4741 cggccaaggg gcacccctga ctggaaatgt gacaacctct cagatggcta acgaacaggg
     4801 actctttgac gtgcactccg tgctgagggt tgtcctggga gctaacggca cctacagctg
     4861 tcttgtgcgc aaccctgtgc tgcaacagga cgcccatggc agcgtgacaa tcacaggcca
     4921 gcctatgacc tttccacctg aggctctgtg ggtcacagtg ggcctgtctg tgtgtctggt
     4981 ggctctgctg gtggcactgg ccttcgtgtg ttggcggaag atcaagcaga gctgcgagga
     5041 agaaaacgct ggcgccgaag atcaggatgg cgaaggcgag ggaagcaaga cagcactgca
     5101 gcctctgaag cacagcgaca gcaaagagga cgacggacaa gaactggccc gcggccgcga
     5161 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
     5221 caagtaggcc tgcgatatcc agcggatccc gcccctctcc ctcccccccc cctaacgtta
     5281 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     5341 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     5401 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     5461 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     5521 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     5581 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     5641 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     5701 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     5761 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     5821 gataagcttg ccacaaccca caaggagacg accttccatg accgagtaca agcccacggt
     5881 gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc
     5941 cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga
     6001 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     6061 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6121 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6181 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6241 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6301 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     6361 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     6421 gcgcacctgg tgcatgaccc gcaagcccgg tgcctagacg cgtctggaac aatcaacctc
     6481 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6541 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6601 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6661 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6721 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6781 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6841 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     6901 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6961 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7021 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
     7081 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     7141 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     7201 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag
     7261 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     7321 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     7381 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     7441 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     7501 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     7561 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     7621 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     7681 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     7741 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7801 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7861 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     7921 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
     7981 gctagcgttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     8041 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8101 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8161 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8221 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8281 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8341 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8401 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8461 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8521 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8581 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8641 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8701 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8761 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8821 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8881 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8941 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9001 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9061 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9121 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9181 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9241 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9301 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9361 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9421 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9481 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9541 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9601 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9661 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9721 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9781 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9841 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9901 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9961 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10021 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10081 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10141 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
    10201 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    10261 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    10321 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    10381 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    10441 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    10501 gt
//
