LOCUS       V014747                10034 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014747
VERSION     V014747
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2338..3519
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             4691..4756
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     misc_feature    4784..5370
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5390..5986
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6003..6591
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6798..7431
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7607..7637)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7652..7673)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7961..8549)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8723..9580)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9581..9685)
                     /label="AmpR promoter"
     polyA_signal    9733..9867
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtacgaatt
     3541 cgccaccatg acactgctgt ggctgactct gctgctggtg gccacacctt gcctgctgag
     3601 gaccaaagag gaccccaacg ctcccatccg gaacctgcgg atgaaggaaa aagcccagca
     3661 gctgatgtgg gacctgaacc ggaatgtgac cgacgtggaa tgcatcaagg gcaccgacta
     3721 cagcatgccc gccatgaaca acagctactg ccagttcggc gccatcagcc tgtgcgaagt
     3781 gaccaactac accgtcagag tggcctctcc acctttcagc acctggattc tgttccccga
     3841 gaacagcggc acacctagag ccggcgctga gaatctgaca tgctgggtgc acgacgtgga
     3901 ctttctgagc tgttcttggg tcgtgggacc agccgctcct gccgatgttc agtacgacct
     3961 gtacctgaac aaccccaact ctcacgagca gtaccggtgc ctgcactaca agacagatgc
     4021 cagaggcacc cagatcggct gcagattcga tgatatcgcc cctctgagca ggggcagcca
     4081 gtctagccac attctcgtgc ggggaagatc tgccgccgtg tctatccctt gcaccgacaa
     4141 gttcgtgttc ttcagccaga tcgagcggct gacccctcct aatatgaccg gcgagtgcaa
     4201 cgagacacac agcttcatgc actggaagat gaagtcccac ttcaaccgga agttcagata
     4261 cgagctgcgg atccagaaac ggatgcagcc agtgcggaca gagcaagtgc gggacaccac
     4321 aagcttccag ctgcctaatc ctggcaccta cacagtgcag atccgggcca gagaaaccgt
     4381 gtacgagttc ctgagcgcct ggtctacccc tcagagattc gagtgcgacc aagaggaagg
     4441 cgcctctagc agagcttgga gaacctctct gctgatcgcc ctgggaacac tgctggctct
     4501 gctgtgcgtg ttcctgatct gtcggagata cctggtcatg cagcggctgt tccctcggat
     4561 ccctcacatg aaggacccca tcggcgatac cttccagcag gacaagctgg ttgtgtggga
     4621 ggccggaaaa gccggcctgg aagaatgtct ggtgtccgag gtgcaggtcg tggaaaagac
     4681 acgcggccgc gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     4741 ggatgacgat gacaagtagg cctgcgatat ccagcggatc ccgcccctct ccctcccccc
     4801 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     4861 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     4921 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     4981 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     5041 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     5101 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     5161 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     5221 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     5281 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     5341 gaaaaacacg atgataagct tgccacaacc cacaaggaga cgaccttcca tgaccgagta
     5401 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
     5461 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
     5521 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     5581 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     5641 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     5701 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     5761 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     5821 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     5881 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     5941 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctaga cgcgtctgga
     6001 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6061 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6121 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6181 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6241 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6301 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6361 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     6421 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6481 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     6541 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta
     6601 attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     6661 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     6721 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6781 aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     6841 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     6901 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     6961 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     7021 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     7081 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     7141 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     7201 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     7261 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7321 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7381 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     7441 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     7501 ggccttgaca ttgctagcgt ttaccgtcga cctctagcta gagcttggcg taatcatggt
     7561 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7621 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7681 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7741 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7801 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7861 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7921 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7981 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8041 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8101 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8161 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8221 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8281 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8341 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8401 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8461 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8521 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8581 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8641 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8701 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8761 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8821 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8881 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8941 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9001 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9061 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9121 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9181 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9241 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9301 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9361 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9421 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9481 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9541 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9601 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9661 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     9721 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9781 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9841 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
     9901 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
     9961 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    10021 acaaacttag tagt
//
