LOCUS       V014798                 9246 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014798
VERSION     V014798
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2807..3499
                     /label="TurboRFP"
                     /note="red fluorescent protein from Entacmaea quadricolor"
     CDS             3527..3553
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             3557..3583
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             3587..3613
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     promoter        3655..4154
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4175..5197
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     misc_feature    5214..5802
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6009..6642
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6771..6787)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6795..6811)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6819..6849)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6864..6885)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7173..7761)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7935..8792)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8793..8897)
                     /label="AmpR promoter"
     polyA_signal    8945..9079
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctaccggtc gccaccatga gcgagctgat
     2821 caaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa
     2881 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga agatcaaggt
     2941 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg
     3001 cagcaaagcc ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc
     3061 tgagggcttc acatgggaga gaatcaccac atacgaagac gggggcgtgc tgaccgctac
     3121 ccaggacacc agcttccaga acggctgcat catctacaac gtcaagatca acggggtgaa
     3181 cttcccatcc aacggccctg tgatgcagaa gaaaacacgc ggctgggagg ccaacaccga
     3241 gatgctgtac cccgctgacg gcggcctgag aggccacagc cagatggccc tgaagctcgt
     3301 gggcgggggc tacctgcact gctccttcaa gaccacatac agatccaaga aacccgctaa
     3361 gaacctcaag atgcccggct tccacttcgt ggaccacaga ctggaaagaa tcaaggaggc
     3421 cgacaaagag acctacgtcg agcagcacga gatggctgtg gccaagtact gcgacctccc
     3481 tagcaaactg gggcacagag cggccgctca gagtgggccc ctcgagtacc catacgacgt
     3541 acctgattat gcaacttatc cttatgatgt acctgattat gctagctatc cttatgatgt
     3601 cccagactac gcatagggat ccaccggatc tagataactg atcataattc taccgggtag
     3661 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     3721 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     3781 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     3841 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     3901 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     3961 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     4021 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     4081 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     4141 ctccgggcct ttcgacctgc agcccaagct taccatgaaa aagcctgaac tcaccgcgac
     4201 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     4261 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     4321 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     4381 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     4441 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     4501 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     4561 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     4621 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     4681 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     4741 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     4801 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     4861 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     4921 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     4981 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     5041 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     5101 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     5161 aaaccgacgc cccagcactc gtccgagggc aaaggaatag ccgcgtctgg aacaatcaac
     5221 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     5281 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     5341 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     5401 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     5461 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     5521 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     5581 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     5641 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     5701 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     5761 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag
     5821 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     5881 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     5941 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6001 gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     6061 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     6121 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     6181 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     6241 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     6301 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     6361 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     6421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     6481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     6661 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac
     6721 attgctagcg ttttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     6781 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6841 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6901 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6961 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7021 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7081 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7141 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7201 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7261 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7321 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7381 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7441 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7501 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7561 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7621 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7681 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7741 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7801 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7861 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7921 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7981 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8041 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8101 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8161 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8221 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8281 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8341 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8401 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8461 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8521 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8581 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8641 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8701 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8761 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8821 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8881 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     8941 gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9001 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9061 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     9121 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
     9181 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
     9241 agtagt
//
