LOCUS       V014851                10564 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014851
VERSION     V014851
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10564)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2807..3514
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             3563..5212
                     /label="luciferase"
                     /note="firefly luciferase"
     CDS             5303..5326
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     promoter        5399..5898
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5919..6515
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6532..7120
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7327..7960
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8137..8167)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8182..8203)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8491..9079)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9253..10110)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10111..10215)
                     /label="AmpR promoter"
     polyA_signal    10263..10397
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctaccggtc gccaccatgg tgagcaaggg
     2821 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     2881 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     2941 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     3001 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     3061 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     3121 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     3181 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     3241 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     3301 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     3361 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     3421 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     3481 ccactccacc ggcggcatgg acgagctgta caagtccgga ctcagatctc gaggaggtgg
     3541 aggtagtgga ggaggcggat caatggaaga cgccaaaaac ataaagaaag gcccggcgcc
     3601 attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata
     3661 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta
     3721 cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
     3781 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
     3841 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
     3901 attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt
     3961 gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga
     4021 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
     4081 tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc
     4141 actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag
     4201 aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
     4261 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
     4321 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt
     4381 tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc
     4441 cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc
     4501 ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct
     4561 gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc
     4621 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt
     4681 tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag
     4741 aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga
     4801 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt
     4861 catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga
     4921 attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc
     4981 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat
     5041 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg
     5101 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag
     5161 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgggtggggg
     5221 cggttcaggt ggcggtggat ccgcggccgc atgcacgcgt gactacaaag accatgacgg
     5281 tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtagg aattctgcag
     5341 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata attctaccgg
     5401 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     5461 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     5521 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     5581 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     5641 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     5701 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     5761 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     5821 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     5881 tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg
     5941 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     6001 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     6061 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     6121 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     6181 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     6241 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     6301 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     6361 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     6421 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     6481 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct
     6541 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6601 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6661 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6721 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6781 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6841 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6901 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     6961 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7021 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7081 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc
     7141 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     7201 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     7261 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga
     7321 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     7381 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
     7441 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
     7501 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
     7561 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
     7621 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
     7681 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
     7741 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
     7801 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7861 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7921 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     7981 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat
     8041 tgctagcgtt ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     8101 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8161 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8221 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8281 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8341 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8401 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8461 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8521 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8581 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8641 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8701 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8761 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8821 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8881 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8941 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9001 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9061 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9121 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9181 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9241 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9301 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9361 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9421 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9481 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9541 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9601 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9661 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9721 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9781 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9841 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9901 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9961 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10021 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10081 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10141 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10201 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
    10261 tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    10321 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    10381 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
    10441 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
    10501 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    10561 tagt
//
