LOCUS       V029837                 8257 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029837
VERSION     V029837
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     promoter        2206..2520
                     /note="Tet-responsive promoter&nbsp;PTight, consisting of
                     seven tet operator&nbsp;sequences followed by the minimal
                     CMV promoter"
                     /label="tight TRE promoter"
     CDS             2548..4161
                     /label="RBBP5(NM_005057)"
                     /note="RBBP5(NM_005057)"
                     /gene="RBBP5"
     CDS             complement(4645..4656)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /transl_table=1
                     /translation="IEGR"
                     /label="Factor Xa site"
     LTR             4975..5608
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(5737..5753)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5761..5777
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5785..5815)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5830..5851
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6139..6724)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6895..7755)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7756..7860)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    7908..8042
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
     2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2521 tggagaagga tccgcggccg cgccaccatg aacctcgagt tgctggagtc ctttgggcag
     2581 aactatccag aggaagctga tggaactttg gattgtatca gcatggcttt gacttgcacc
     2641 tttaacaggt ggggcacact gcttgcagtt ggctgtaatg atggccgaat tgtcatctgg
     2701 gatttcttga caagaggcat tgctaaaata attagtgcac acatccatcc agtgtgttct
     2761 ttatgctgga gccgagatgg tcataaactc gtgagtgctt ccactgataa catagtgtca
     2821 cagtgggatg ttctttcagg cgactgtgac cagaggtttc gattcccttc acccatctta
     2881 aaagtccaat atcatccacg agatcagaac aaggttctcg tgtgtcccat gaaatctgct
     2941 cctgtcatgt tgaccctttc agattccaaa catgttgttc tgccggtgga cgatgactcc
     3001 gatttgaacg ttgtggcatc ttttgatagg cgaggggaat atatttatac gggaaacgca
     3061 aaaggcaaga ttttggtcct aaaaacagat tctcaggatc ttgttgcttc cttcagagtg
     3121 acaactggaa caagcaatac cacagccatt aagtcaattg agtttgcccg gaaggggagt
     3181 tgctttttaa ttaacacggc agatcgaata atcagagttt atgatggcag agaaatctta
     3241 acatgtggaa gagatggaga gcctgaacct atgcagaaat tgcaggattt ggtgaatagg
     3301 accccatgga agaaatgttg tttctctggg gatggggaat acatcgtggc aggttctgcc
     3361 cggcagcatg ccctgtacat ctgggagaag agcattggca acctggtgaa gattctccat
     3421 gggacgagag gagaactcct cttggatgta gcttggcatc ctgttcgacc catcatagca
     3481 tccatttcca gtggagtggt atctatctgg gcacagaatc aagtagaaaa ctggagtgca
     3541 tttgcaccag acttcaaaga attggatgaa aatgtagaat acgaagaaag ggaatcagag
     3601 tttgatattg aagatgaaga taagagtgag cctgagcaga caggggctga tgctgcagaa
     3661 gatgaggaag tggatgtcac cagcgtggac cctattgctg ccttctgtag cagtgatgaa
     3721 gagctggaag attcaaaggc tctattgtat ttacccattg cccctgaggt agaagaccca
     3781 gaagaaaatc cttacggccc cccaccggat gcagtccaaa cctccttgat ggatgaaggg
     3841 gctagttcag agaagaagag gcagtcctca gcagatgggt cccagccacc taagaagaaa
     3901 cccaaaacaa ccaatataga acttcaagga gtaccaaatg atgaagtcca tccactactg
     3961 ggtgtgaagg gggatggcaa atccaagaag aagcaagcag gccggcctaa aggatcaaaa
     4021 ggtaaagaga aagattctcc atttaaaccg aaactctaca aaggggacag aggtttacct
     4081 ctggaaggat cagcgaaggg taaagtgcag gcggaactca gccagccctt gacagcagga
     4141 ggagcaatct cagaactgtt atgaacgcgt gaattccaac ctctggatta caaaatttgt
     4201 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4261 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4321 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4381 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4441 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4501 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4561 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     4621 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4681 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4741 tccctttggg ccgcctcccc gcctggaagt ttaaacctgc agtcgagacc tagaaaaaca
     4801 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     4861 acaagaggag gaggaggtgg gtttttccag tcacacctca ggtaccttta agaccaatga
     4921 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctgctggaag
     4981 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     5041 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     5101 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     5161 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     5221 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     5281 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     5341 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     5401 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     5461 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5521 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5581 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     5641 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgtttt
     5701 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5761 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5821 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5881 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5941 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6001 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6061 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6121 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6181 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6241 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6301 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6361 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6421 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6481 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6541 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6601 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6661 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6721 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     6781 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     6841 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     6901 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     6961 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7021 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7081 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7141 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7201 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7261 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7321 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7381 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7441 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7501 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7561 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7621 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     7681 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     7741 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     7801 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     7861 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg
     7921 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7981 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     8041 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
     8101 ccactgccaa ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt
     8161 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt ttttaaagaa
     8221 attgtatttg ttaaatatgt actacaaact tagtagt
//
