LOCUS       V025745                10308 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025745
VERSION     V025745
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..4938
                     /label="ZRANB1(NM_017580)"
                     /note="ZRANB1(NM_017580)"
                     /gene="ZRANB1"
     CDS             4945..4974
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     misc_feature    5011..5597
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             5617..6216
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6230..6818
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7025..7658
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7786..7802)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7810..7826
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7834..7864)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7879..7900
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8188..8776)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8947..9807)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9808..9912)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9960..10094
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgtca
     2821 gaacgtggaa ttaagtgggc ttgtgaatat tgtacgtatg aaaactggcc atctgcaatc
     2881 aagtgtacta tgtgtcgtgc ccaaagacct agtggaacaa ttattacaga agatccattt
     2941 aaaagtggtt caagtgatgt tggtagagat tgggatcctt ccagcaccga aggaggaagt
     3001 agtcctttga tatgtccaga ctctagtgca agaccaaggg tgaaatcttc gtatagcatg
     3061 gaaaatgcaa ataagtggtc atgccacatg tgtacatatt tgaactggcc aagagcaatc
     3121 agatgtaccc agtgcttatc ccaacgtagg accaggagtc ctacagaatc tcctcagtcc
     3181 tcaggatctg gctcaagacc agttgctttt tctgttgatc cttgtgagga atacaatgat
     3241 agaaataaac tgaacactag gacacagcac tggacttgct ctgtttgcac atatgaaaac
     3301 tgggccaagg ctaaaagatg tgttgtttgt gatcatccca gacctaataa cattgaagca
     3361 atagaattgg cagagactga agaggcttct tcaataataa atgagcaaga cagagctcga
     3421 tggaggggaa gttgcagtag tggtaatagc caaaggagat cacctcctgc tacgaagcgg
     3481 gactctgaag tgaaaatgga ttttcagagg attgaattgg ctggtgctgt gggaagcaag
     3541 gaggaacttg aagtagactt taaaaaacta aagcaaatta aaaacaggat gaaaaagact
     3601 gattggctct tcctcaatgc ttgtgtgggg gttgtagaag gtgatttagc tgccatagaa
     3661 gcatacaagt catcaggagg agacattgca cgtcagctca ccgcagatga agtacgcttg
     3721 ctgaatcgtc cttctgcctt tgatgttggc tatactcttg tacacttggc tatacgtttt
     3781 cagaggcagg atatgctagc aatattgctt acagaggtgt ctcaacaagc agcaaagtgt
     3841 attccagcaa tggtgtgtcc tgaactgaca gaacaaatcc ggagagagat agctgcctct
     3901 cttcatcaga gaaaggggga ttttgcttgc tattttctga ctgaccttgt aacatttaca
     3961 ttgccagcag atattgaaga tttgccccca acagtccaag aaaaattatt tgatgaggtg
     4021 cttgatagag acgttcaaaa agaattagaa gaagaatctc caattattaa ctggtccttg
     4081 gaattggcta cacgtttgga cagtcgactg tatgcacttt ggaaccggac tgcaggagac
     4141 tgcctacttg attcagttct acaagctacc tggggcatct atgacaagga ctcagtgctt
     4201 cggaaagccc tgcatgacag cctgcatgac tgttcacatt ggttttacac acgctggaaa
     4261 gattgggaat catggtattc tcagagcttt ggtttacatt tttccttgag agaagaacag
     4321 tggcaagaag actgggcatt tatactctct cttgctagtc agcctggagc aagcttggag
     4381 cagacgcaca tttttgtact ggcacatatt cttagacgac caattatagt ttatggagta
     4441 aaatattaca agagtttccg gggagaaact ttaggatata ctcggtttca aggtgtttat
     4501 ctgcctttgt tgtgggaaca gagtttttgt tggaaaagtc cgattgctct gggttatacg
     4561 aggggccact tctctgcttt ggttgccatg gaaaatgatg gctatggcaa ccgaggtgct
     4621 ggtgctaatc tcaataccga tgatgatgtc accatcacat ttttgcctct ggttgacagt
     4681 gaaaggaagc tactccatgt gcacttcctt tctgctcagg agctaggtaa tgaggaacag
     4741 caagaaaaac tgctcaggga gtggctggac tgctgtgtga cggagggggg agttctggtt
     4801 gccatgcaga agagttctcg gcggcgaaat caccccctgg tcactcagat ggtagaaaaa
     4861 tggcttgacc gctaccgaca gatccggccg tgtacatccc tgtctgatgg agaggaagat
     4921 gaggatgatg aagatgaact cgaggaacaa aaactcatct cagaagagga tctgtgatcg
     4981 agactagttc tagagcggcc gcggatcccg cccctctccc tccccccccc ctaacgttac
     5041 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     5101 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     5161 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     5221 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     5281 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     5341 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     5401 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     5461 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     5521 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     5581 ataagcttgc cacaacccac aaggagacga ccttccatga ccgagtacaa gcccacggtg
     5641 cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc
     5701 gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag
     5761 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     5821 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     5881 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     5941 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     6001 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     6061 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     6121 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     6181 cgcacctggt gcatgacccg caagcccggt gcctagacgc gtctggaaca atcaacctct
     6241 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6301 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6361 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6421 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6481 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6541 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6601 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     6661 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6721 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6781 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga
     6841 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     6901 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     6961 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg
     7021 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     7081 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     7141 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     7201 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     7261 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     7321 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     7381 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     7441 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     7501 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7561 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7621 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7681 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg
     7741 ctagcgttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     7801 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7861 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7921 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7981 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8041 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8101 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8161 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8221 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8281 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8341 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8401 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8461 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8521 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8581 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8641 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8701 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8761 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8821 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8881 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8941 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9001 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9061 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9121 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9181 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9241 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9301 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9361 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9421 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9481 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9541 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9601 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9661 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9721 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9781 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9841 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9901 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca
     9961 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10021 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10081 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    10141 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    10201 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    10261 tttttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
