LOCUS       V026353                12402 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026353
VERSION     V026353
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..7032
                     /label="USP6(NM_001304284)"
                     /note="USP6(NM_001304284)"
                     /gene="USP6"
     CDS             7039..7068
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     misc_feature    7105..7691
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             7711..8310
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8324..8912
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9119..9752
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9880..9896)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9904..9920
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9928..9958)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9973..9994
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10282..10870)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11041..11901)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11902..12006)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    12054..12188
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggac
     2821 atggtagaga atgcagatag tttgcaggca caggagcgga aggacatact tatgaagtat
     2881 gacaagggac accgagctgg gctgccagag gacaaggggc ctgagcccgt tggaatcaac
     2941 agcagcattg atcgttttgg cattttgcat gagacggagc tgcctcctgt gactgcacgg
     3001 gaggcgaaga aaattcggcg ggagatgaca cgaacgagca agtggatgga aatgctggga
     3061 gaatgggaga catataagca cagtagcaaa ctcatagatc gagtgtacaa gggaattccc
     3121 atgaacatcc ggggcccggt gtggtcagtc ctcctgaaca ttcaggaaat caagttgaaa
     3181 aaccccggaa gataccagat catgaaggag aggggcaaga ggtcatctga acacatccac
     3241 cacatcgacc tggacgtgag gacgactctc cggaaccatg tcttctttag ggatcgatat
     3301 ggagccaagc agagggaact attctacatc ctcctggcct attcggagta taacccggag
     3361 gtgggctact gcagggacct gagccacatc accgccttgt tcctccttta tctgcctgag
     3421 gaggacgcat tctgggcact ggtgcagctg ctggccagtg agaggcactc cctgccagga
     3481 ttccacagcc caaatggtgg gacagtccag gggctccaag accaacagga gcatgtggta
     3541 cccaagtcac aacccaagac catgtggcat caggacaagg aaggtctatg cgggcagtgt
     3601 gcctcgttag gctgccttct ccggaacctg attgacggga tctctctcgg gctcaccctg
     3661 cgcctgtggg acgtgtattt ggtggaagga gaacaggtgt tgatgccaat aaccagcatt
     3721 gctcttaagg ttcagcagaa gcgcctcatg aagacatcca ggtgtggcct gtgggcacgt
     3781 ctgcggaacc aattcttcga tacctgggcc atgaacgatg acaccgtgct caagcatctt
     3841 agggcctcta cgaagaaact aacaaggaag caaggggacc tgccaccccc agccaaacgc
     3901 gagcaagggt ccttggcacc caggcctgtg ccggcttcac gtggtgggaa gaccctctgc
     3961 aaggggtata ggcaggcccc tccaggccca ccagcccagt tccagcggcc catttgctca
     4021 gcttccccgc catgggcatc tcgtttttcc acgccctgtc ctggtggggc tgtccgggaa
     4081 gacacgtacc ctgtgggcac tcagggtgtg cccagcctgg ccctggctca gggaggacct
     4141 cagggttcct ggagattcct ggagtggaag tcaatgcccc ggctcccaac ggacctggat
     4201 atagggggcc cttggttccc ccattatgat tttgaatgga gctgctgggt ccgtgccata
     4261 tcccaggagg accagctggc cacctgctgg caggctgaac actgcggaga ggttcacaac
     4321 aaagatatga gttggcctga ggagatgtct tttacagcaa atagtagtaa aatagataga
     4381 caaaaggttc ccacagaaaa gggagccaca ggtctaagca acctgggaaa cacatgcttc
     4441 atgaactcaa gcatccagtg cgttagtaac acacagccac tgacacagta ttttatctca
     4501 gggagacatc tttatgaact caacaggaca aatcccattg gtatgaaggg gcatatggct
     4561 aaatgctatg gtgatttagt gcaggaactc tggagtggaa ctcagaagag tgttgcccca
     4621 ttaaagcttc ggcggaccat agcaaaatat gctcccaagt ttgatgggtt tcagcaacaa
     4681 gactcccaag aacttctggc ttttctcttg gatggtcttc atgaagatct caaccgagtc
     4741 catgaaaagc catatgtgga actgaaggac agtgatggcc gaccagactg ggaagtagct
     4801 gcagaggcct gggacaacca tctaagaaga aatagatcaa ttattgtgga tttgttccat
     4861 gggcagctaa gatctcaagt caaatgcaag acatgtgggc atataagtgt ccgatttgac
     4921 cctttcaatt ttttgtcttt gccactacca atggacagtt acatggactt agaaataaca
     4981 gtgattaagt tagatggtac tacccctgta cggtatggac taagactgaa tatggatgaa
     5041 aagtacacag gtttaaaaaa acagctgagg gatctctgtg gacttaattc agaacaaatc
     5101 ctactagcag aagtacatga ttccaacata aagaactttc ctcaggataa ccaaaaagta
     5161 caactctcag tgagcggatt tttgtgtgca tttgaaattc ctgtcccttc atctccaatt
     5221 tcagcttcta gtccaacaca aatagatttc tcctcttcac catctacaaa tggaatgttc
     5281 accctaacta ccaatgggga cctacccaaa ccaatattca tccccaatgg aatgccaaac
     5341 actgttgtgc catgtggaac tgagaagaac ttcacaaatg gaatggttaa tggtcacatg
     5401 ccatctcttc ctgacagccc ctttacaggt tacatcattg cagtccaccg aaaaatgatg
     5461 aggacagaac tgtatttcct gtcacctcag gagaatcgcc ccagcctctt tggaatgcca
     5521 ttgattgttc catgcactgt gcatacccgg aagaaagacc tatatgatgc ggtttggatt
     5581 caagtatcct ggttagcaag accactccca cctcaggaag ctagtattca tgcccaggat
     5641 cgtgataact gtatgggcta tcaatatcca ttcactctac gagttgtgca gaaagatggg
     5701 aactcctgtg cttggtgccc acagtataga ttttgcagag gctgtaaaat tgattgtggg
     5761 gaagacagag ctttcattgg aaatgcctat attgctgtgg attggcaccc cacagccctt
     5821 caccttcgct atcaaacatc ccaggaaagg gttgtagata agcatgagag tgtggagcag
     5881 agtcggcgag cgcaagccga gcccatcaac ctggacagct gtctccgtgc tttcaccagt
     5941 gaggaagagc taggggaaag tgagatgtac tactgttcca agtgtaagac ccactgctta
     6001 gcaacaaaga agctggatct ctggaggctt ccacccttcc tgattattca ccttaagcga
     6061 tttcaatttg taaatgatca gtggataaaa tcacagaaaa ttgtcagatt tcttcgggaa
     6121 agttttgatc cgagtgcttt tttggtacca cgagacccgg ccctctgcca gcataaacca
     6181 ctcacacccc agggggatga gctctccaag cccaggattc tggcaagaga ggtgaagaaa
     6241 gtggatgcgc agagttcggc tggaaaagag gacatgctcc taagcaaaag cccatcctca
     6301 ctcagcgcta acatcagcag cagcccaaaa ggttctcctt cttcatcaag aaaaagtgga
     6361 accagctgtc cctccagcaa aaacagcagc cctaatagca gcccacggac tttggggagg
     6421 agcaaaggga ggctccggct gccccagatt ggcagcaaaa ataagccgtc aagtagtaag
     6481 aagaacttgg atgccagcaa agagaatggg gctgggcaga tctgtgagct ggctgacgcc
     6541 ttgagccgag ggcatatgcg ggggggcagc caaccagagc tggtcactcc tcaggaccat
     6601 gaggtagctt tggccaatgg attcctttat gagcatgaag catgtggcaa tggctgtggc
     6661 gatggctaca gcaatggtca gcttggaaac cacagtgaag aagacagcac tgatgaccaa
     6721 agagaagaca ctcatattaa gcctatttat aatctatatg caatttcatg ccattcagga
     6781 attctgagtg ggggccatta catcacttat gccaaaaacc caaactgcaa gtggtactgt
     6841 tataatgaca gcagctgtga ggaacttcac cctgatgaaa ttgacaccga ctctgcctac
     6901 attcttttct atgagcagca ggggatagac tacgcacaat ttctgccaaa gattgatggc
     6961 aaaaagatgg cagacacaag cagtacggat gaagactctg agtctgatta cgaaaagtac
     7021 tctatgttac agctcgagga acaaaaactc atctcagaag aggatctgtg atcgagacta
     7081 gttctagagc ggccgcggat cccgcccctc tccctccccc ccccctaacg ttactggccg
     7141 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     7201 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     7261 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     7321 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     7381 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     7441 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     7501 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     7561 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     7621 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc
     7681 ttgccacaac ccacaaggag acgaccttcc atgaccgagt acaagcccac ggtgcgcctc
     7741 gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac
     7801 cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa
     7861 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     7921 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     7981 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     8041 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     8101 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     8161 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     8221 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     8281 tggtgcatga cccgcaagcc cggtgcctag acgcgtctgg aacaatcaac ctctggatta
     8341 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     8401 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     8461 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     8521 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     8581 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     8641 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     8701 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     8761 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     8821 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     8881 gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta
     8941 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     9001 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     9061 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg
     9121 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     9181 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     9241 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
     9301 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     9361 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
     9421 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
     9481 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     9541 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     9601 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9661 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9721 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     9781 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg
     9841 tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     9901 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     9961 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    10021 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    10081 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    10141 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    10201 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    10261 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    10321 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    10381 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    10441 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    10501 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    10561 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    10621 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    10681 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    10741 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    10801 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10861 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10921 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10981 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    11041 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    11101 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    11161 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    11221 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    11281 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    11341 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    11401 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    11461 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    11521 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    11581 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    11641 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    11701 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    11761 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    11821 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    11881 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11941 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    12001 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt
    12061 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    12121 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    12181 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
    12241 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
    12301 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttttta
    12361 aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
