LOCUS       V022512                11119 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022512
VERSION     V022512
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2809..2818
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2818..2847
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2854..5796
                     /label="USP15(NM_001252078)"
                     /note="USP15(NM_001252078)"
                     /gene="USP15"
     misc_feature    5822..6408
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             6428..7027
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7041..7629
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7836..8469
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8597..8613)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8621..8637
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8645..8675)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8690..8711
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8999..9587)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9758..10618)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10619..10723)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10771..10905
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggaa
     2821 caaaaactca tctcagaaga ggatctgctc gagatggcgg aaggcggagc ggcggatctg
     2881 gacacccagc ggtctgacat cgcgacgctg ctcaaaacct cgctccggaa aggggacacc
     2941 tggtacctag tcgatagtcg ctggttcaaa cagtggaaaa aatatgttgg ctttgacagt
     3001 tgggacaaat accagatggg agatcaaaat gtgtatcctg gacccattga taactctgga
     3061 cttctcaaag atggtgatgc ccagtcactt aaggaacacc ttattgatga attggattac
     3121 atactgttgc caactgaagg ttggaataaa cttgtcagct ggtacacatt gatggaaggt
     3181 caagagccaa tagcacgaaa ggtggttgaa cagggtatgt ttgtaaagca ctgcaaagta
     3241 gaagtatatc tcacagaatt gaagctatgt gaaaatggaa acatgaataa tgttgtaact
     3301 cgaagattta gcaaagctga cacaatagat acaattgaaa aggaaataag aaaaatcttc
     3361 agtattccag atgaaaagga gaccagattg tggaacaaat acatgagtaa cacatttgaa
     3421 ccactgaata aaccagacag caccattcag gatgctggtt tataccaagg acaggtatta
     3481 gtgatagaac agaaaaatga agatggaaca tggccaaggg gtccttctac tcctaagtcc
     3541 ccaggtgcat ccaatttttc aactttacca aagatctctc cttcatctct atcaaataat
     3601 tataacaaca tgaacaacag aaatgtgaaa aactcaaatt actgtcttcc atcatatacc
     3661 gcttataaga actatgatta ttcggaacct ggaagaaaca atgaacagcc aggcctctgt
     3721 ggcctaagta acttgggaaa tacgtgtttc atgaactcag ctattcagtg tttgagcaac
     3781 acacctccac ttactgagta tttcctcaat gataagtatc aagaagaact gaattttgac
     3841 aatcccttag gaatgagagg tgaaatagct aaatcttatg ccgaactgat caagcaaatg
     3901 tggtctggaa agtttagcta cgtcacccca agagccttta agacacaggt aggacgtttt
     3961 gcacctcagt tctctggata tcagcagcaa gactgtcaag aactgttagc tttcctatta
     4021 gatggattac atgaggattt gaatagaatt aggaaaaaac catatataca attaaaagat
     4081 gcagatggaa ggccagataa ggtggttgcc gaagaagcct gggaaaacca tttaaaacga
     4141 aatgattcta tcatagtaga tatatttcat ggccttttca aatcaacttt agtttgtcct
     4201 gagtgtgcta agatttcagt aacatttgat cctttttgtt acttgacact tccattgccc
     4261 atgaaaaaag aacgcacctt ggaagtttac ttagttagaa tggatccact taccaaacct
     4321 atgcagtaca aagtggttgt ccccaaaatt ggaaacatat tagatctttg tacagcattg
     4381 tctgctttgt caggaatacc tgcagataag atgatagtta ctgatatata caatcataga
     4441 tttcacagaa tattcgctat ggatgaaaac cttagtagta ttatggaacg ggatgatatt
     4501 tatgtgtttg aaattaacat caataggaca gaagatacag agcacgtgat tattcctgtt
     4561 tgcctaagag aaaaattcag acactcgagt tatacccacc atactggttc ttcacttttt
     4621 ggtcagccct ttcttatggc tgtaccacga aacaatactg aagacaaact ttataatctc
     4681 ctgctcttga gaatgtgccg atatgtcaaa atatctactg aaactgaaga aactgaagga
     4741 tccctacact gctgtaagga ccaaaatatt aatgggaatg gcccaaatgg catacatgaa
     4801 gaaggctcac caagtgaaat ggaaacagat gagccagatg atgaatccag ccaggatcaa
     4861 gaacttccct cagagaatga aaacagtcag tctgaagatt cagttggagg agataatgat
     4921 tctgaaaatg gattatgtac tgaggatact tgcaaaggtc aactcacggg acacaaaaaa
     4981 cgattgttta cattccagtt caacaactta ggcaatactg atatcaacta catcaaagat
     5041 gataccaggc atataagatt tgatgatagg cagcttaggc tagatgaaag atcttttctt
     5101 gctttggatt gggatcctga tttgaaaaaa agatattttg atgaaaatgc tgctgaggac
     5161 tttgaaaaac atgaaagtgt ggagtataaa cctcctaaaa aaccctttgt gaaattaaaa
     5221 gattgcattg aactttttac aacaaaagaa aagctaggtg ctgaagatcc ctggtattgt
     5281 ccgaattgta aagaacatca gcaagccaca aagaaattgg atttatggtc cctgcctcca
     5341 gtacttgtag tacatctcaa gcgattttct tacagtcgat acatgagaga caagttggat
     5401 accttagttg attttcctat caatgacttg gatatgtcgg aattcttaat taatccaaat
     5461 gcaggtcctt gccgctataa tctgattgct gtttccaacc actatggagg gatgggagga
     5521 ggacactata ctgcttttgc aaaaaataaa gatgatggaa aatggtacta ttttgatgac
     5581 agtagtgtct ccactgcatc tgaagaccaa attgtgtcca aagcagcata tgtactcttc
     5641 taccagagac aagacacttt cagtggaact ggcttttttc ctcttgaccg agaaactaaa
     5701 ggtgcttcag ctgccactgg catcccatta gaaagtgatg aagatagcaa tgataatgac
     5761 aatgatatag aaaatgaaaa ctgtatgcac actaactaat ctagagcggc cgcggatccc
     5821 gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     5881 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     5941 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     6001 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     6061 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     6121 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     6181 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     6241 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     6301 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     6361 gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaccca caaggagacg
     6421 accttccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     6481 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     6541 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     6601 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     6661 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     6721 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     6781 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     6841 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     6901 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6961 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     7021 tgcctagacg cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg
     7081 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     7141 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     7201 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7261 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7321 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7381 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     7441 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7501 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7561 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7621 cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag
     7681 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     7741 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     7801 agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg
     7861 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     7921 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     7981 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     8041 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     8101 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     8161 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     8221 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     8281 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8341 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8401 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8461 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     8521 gaatatcaga gagtgagagg ccttgacatt gctagcgttt accgtcgacc tctagctaga
     8581 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8641 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8701 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8761 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8821 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8881 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8941 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     9001 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     9061 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     9121 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     9181 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9241 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9301 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9361 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9421 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9481 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9541 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9601 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9661 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9721 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9781 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9841 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9901 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9961 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10021 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10081 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    10141 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    10201 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10261 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10321 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10381 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10441 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10501 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10561 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10621 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10681 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10741 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
    10801 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    10861 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    10921 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    10981 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    11041 atttgttaaa tatgtactac aaacttagta gtttttaaag aaattgtatt tgttaaatat
    11101 gtactacaaa cttagtagt
//
