LOCUS V026877 11122 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026877 VERSION V026877 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2818..2844 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2845..5787 /label="USP15(NM_001252078)" /note="USP15(NM_001252078)" /gene="USP15" misc_feature 5825..6411 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES2" CDS 6431..7030 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7044..7632 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7839..8472 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(8600..8616) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8624..8640 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8648..8678) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8693..8714 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9002..9590) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9761..10621) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10622..10726) /gene="bla" /label="AmpR promoter" polyA_signal 10774..10908 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgtac 2821 ccatacgatg ttccagatta cgctatggcg gaaggcggag cggcggatct ggacacccag 2881 cggtctgaca tcgcgacgct gctcaaaacc tcgctccgga aaggggacac ctggtaccta 2941 gtcgatagtc gctggttcaa acagtggaaa aaatatgttg gctttgacag ttgggacaaa 3001 taccagatgg gagatcaaaa tgtgtatcct ggacccattg ataactctgg acttctcaaa 3061 gatggtgatg cccagtcact taaggaacac cttattgatg aattggatta catactgttg 3121 ccaactgaag gttggaataa acttgtcagc tggtacacat tgatggaagg tcaagagcca 3181 atagcacgaa aggtggttga acagggtatg tttgtaaagc actgcaaagt agaagtatat 3241 ctcacagaat tgaagctatg tgaaaatgga aacatgaata atgttgtaac tcgaagattt 3301 agcaaagctg acacaataga tacaattgaa aaggaaataa gaaaaatctt cagtattcca 3361 gatgaaaagg agaccagatt gtggaacaaa tacatgagta acacatttga accactgaat 3421 aaaccagaca gcaccattca ggatgctggt ttataccaag gacaggtatt agtgatagaa 3481 cagaaaaatg aagatggaac atggccaagg ggtccttcta ctcctaagtc cccaggtgca 3541 tccaattttt caactttacc aaagatctct ccttcatctc tatcaaataa ttataacaac 3601 atgaacaaca gaaatgtgaa aaactcaaat tactgtcttc catcatatac cgcttataag 3661 aactatgatt attcggaacc tggaagaaac aatgaacagc caggcctctg tggcctaagt 3721 aacttgggaa atacgtgttt catgaactca gctattcagt gtttgagcaa cacacctcca 3781 cttactgagt atttcctcaa tgataagtat caagaagaac tgaattttga caatccctta 3841 ggaatgagag gtgaaatagc taaatcttat gccgaactga tcaagcaaat gtggtctgga 3901 aagtttagct acgtcacccc aagagccttt aagacacagg taggacgttt tgcacctcag 3961 ttctctggat atcagcagca agactgtcaa gaactgttag ctttcctatt agatggatta 4021 catgaggatt tgaatagaat taggaaaaaa ccatatatac aattaaaaga tgcagatgga 4081 aggccagata aggtggttgc cgaagaagcc tgggaaaacc atttaaaacg aaatgattct 4141 atcatagtag atatatttca tggccttttc aaatcaactt tagtttgtcc tgagtgtgct 4201 aagatttcag taacatttga tcctttttgt tacttgacac ttccattgcc catgaaaaaa 4261 gaacgcacct tggaagttta cttagttaga atggatccac ttaccaaacc tatgcagtac 4321 aaagtggttg tccccaaaat tggaaacata ttagatcttt gtacagcatt gtctgctttg 4381 tcaggaatac ctgcagataa gatgatagtt actgatatat acaatcatag atttcacaga 4441 atattcgcta tggatgaaaa ccttagtagt attatggaac gggatgatat ttatgtgttt 4501 gaaattaaca tcaataggac agaagataca gagcacgtga ttattcctgt ttgcctaaga 4561 gaaaaattca gacactcgag ttatacccac catactggtt cttcactttt tggtcagccc 4621 tttcttatgg ctgtaccacg aaacaatact gaagacaaac tttataatct cctgctcttg 4681 agaatgtgcc gatatgtcaa aatatctact gaaactgaag aaactgaagg atccctacac 4741 tgctgtaagg accaaaatat taatgggaat ggcccaaatg gcatacatga agaaggctca 4801 ccaagtgaaa tggaaacaga tgagccagat gatgaatcca gccaggatca agaacttccc 4861 tcagagaatg aaaacagtca gtctgaagat tcagttggag gagataatga ttctgaaaat 4921 ggattatgta ctgaggatac ttgcaaaggt caactcacgg gacacaaaaa acgattgttt 4981 acattccagt tcaacaactt aggcaatact gatatcaact acatcaaaga tgataccagg 5041 catataagat ttgatgatag gcagcttagg ctagatgaaa gatcttttct tgctttggat 5101 tgggatcctg atttgaaaaa aagatatttt gatgaaaatg ctgctgagga ctttgaaaaa 5161 catgaaagtg tggagtataa acctcctaaa aaaccctttg tgaaattaaa agattgcatt 5221 gaacttttta caacaaaaga aaagctaggt gctgaagatc cctggtattg tccgaattgt 5281 aaagaacatc agcaagccac aaagaaattg gatttatggt ccctgcctcc agtacttgta 5341 gtacatctca agcgattttc ttacagtcga tacatgagag acaagttgga taccttagtt 5401 gattttccta tcaatgactt ggatatgtcg gaattcttaa ttaatccaaa tgcaggtcct 5461 tgccgctata atctgattgc tgtttccaac cactatggag ggatgggagg aggacactat 5521 actgcttttg caaaaaataa agatgatgga aaatggtact attttgatga cagtagtgtc 5581 tccactgcat ctgaagacca aattgtgtcc aaagcagcat atgtactctt ctaccagaga 5641 caagacactt tcagtggaac tggctttttt cctcttgacc gagaaactaa aggtgcttca 5701 gctgccactg gcatcccatt agaaagtgat gaagatagca atgataatga caatgatata 5761 gaaaatgaaa actgtatgca cactaactaa ctcgagacta gttctagagc ggccgcggat 5821 cccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc 5881 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg 5941 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca 6001 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa 6061 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt 6121 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt 6181 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca 6241 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc 6301 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc 6361 acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac ccacaaggag 6421 acgaccttcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 6481 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 6541 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 6601 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 6661 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 6721 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg 6781 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 6841 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 6901 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 6961 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc 7021 cggtgcctag acgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga 7081 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 7141 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 7201 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 7261 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 7321 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 7381 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 7441 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 7501 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 7561 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 7621 ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac 7681 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga 7741 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc 7801 tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca 7861 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta 7921 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa 7981 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt 8041 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag 8101 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa 8161 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg 8221 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt 8281 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 8341 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 8401 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 8461 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 8521 aatgaatatc agagagtgag aggccttgac attgctagcg tttaccgtcg acctctagct 8581 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 8641 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 8701 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 8761 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 8821 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 8881 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 8941 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 9001 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 9061 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 9121 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 9181 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 9241 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 9301 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 9361 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 9421 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 9481 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 9541 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 9601 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 9661 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 9721 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 9781 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 9841 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 9901 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 9961 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 10021 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 10081 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 10141 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 10201 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 10261 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 10321 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 10381 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 10441 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 10501 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 10561 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 10621 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 10681 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 10741 aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt 10801 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 10861 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact 10921 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac 10981 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat 11041 tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt atttgttaaa 11101 tatgtactac aaacttagta gt //