LOCUS V026347 9216 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026347 VERSION V026347 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2815..3846 /label="TRIM44(NM_017583)" /note="TRIM44(NM_017583)" /gene="TRIM44" CDS 3853..3882 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" misc_feature 3919..4505 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES2" CDS 4525..5124 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5138..5726 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5933..6566 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(6694..6710) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6718..6734 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6742..6772) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6787..6808 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7096..7684) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7855..8715) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8716..8820) /gene="bla" /label="AmpR promoter" polyA_signal 8868..9002 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggcc 2821 tctggagtgg gcgcggcctt cgaggaactg cctcacgacg gcacgtgtga cgagtgcgag 2881 cccgacgagg ctccgggggc cgaggaagtg tgccgagaat gcggcttctg ctactgccgc 2941 cgccatgccg aggcgcacag gcagaagttc ctcagtcacc atctggccga atacgtccac 3001 ggctcccagg cctggacccc gccagctgac ggagaggggg cggggaagga agaagcggag 3061 gtcaaggtgg agcaggagag ggagatagaa agcgaggcag gggaagagag tgagtcggag 3121 gaagagagcg agtcagagga agagagcgag acagaggaag agagtgagga tgagagcgat 3181 gaggagagtg aagaagacag cgaggaagaa atggaggatg agcaagaaag cgaggccgaa 3241 gaagacaacc aagaagaagg ggaatccgag gcggagggag aaactgaggc agaaagtgaa 3301 tttgacccag aaatagaaat ggaagcagag agagtggcca agaggaagtg tccggaccat 3361 gggcttgatt tgagtaccta ttgccaggaa gataggcagc tcatctgtgt cctgtgtcca 3421 gtcattgggg ctcaccaggg ccaccaactc tccaccctag acgaagcctt tgaagaatta 3481 agaagcaaag actcaggtgg actgaaggcc gctatgatcg aattggtgga aaggttgaag 3541 ttcaagagct cagaccctaa agtaactcgg gaccaaatga agatgtttat acagcaggaa 3601 tttaagaaag ttcagaaagt gattgctgat gaggagcaga aggcccttca tctagtggac 3661 atccaagagg caatggccac agctcatgtg actgagatac tggcagacat ccaatcccac 3721 atggataggt tgatgactca gatggcccaa gccaaggaac aacttgatac ctctaatgaa 3781 tcagctgagc caaaggcaga gggcgatgag gaaggaccca gtggtgccag tgaagaagag 3841 gacacactcg aggaacaaaa actcatctca gaagaggatc tgtgatcgag actagttcta 3901 gagcggccgc ggatcccgcc cctctccctc ccccccccct aacgttactg gccgaagccg 3961 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt 4021 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct 4081 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct 4141 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc 4201 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc 4261 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc 4321 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc 4381 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct 4441 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca 4501 caacccacaa ggagacgacc ttccatgacc gagtacaagc ccacggtgcg cctcgccacc 4561 cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 4621 acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 4681 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 4741 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 4801 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 4861 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 4921 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 4981 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 5041 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 5101 atgacccgca agcccggtgc ctagacgcgt ctggaacaat caacctctgg attacaaaat 5161 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 5221 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 5281 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 5341 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 5401 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 5461 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 5521 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 5581 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 5641 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg 5701 gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa 5761 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa 5821 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg 5881 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg 5941 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc 6001 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt 6061 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga 6121 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa 6181 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat 6241 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac 6301 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata 6361 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga 6421 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 6481 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 6541 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 6601 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc 6661 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 6721 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 6781 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 6841 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 6901 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 6961 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 7021 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 7081 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 7141 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 7201 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 7261 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 7321 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 7381 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 7441 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 7501 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 7561 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7621 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 7681 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 7741 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 7801 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 7861 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 7921 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 7981 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 8041 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 8101 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8161 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 8221 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 8281 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 8341 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 8401 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 8461 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 8521 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 8581 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 8641 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 8701 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 8761 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 8821 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg 8881 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 8941 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 9001 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta 9061 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt 9121 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt tttaaagaaa 9181 ttgtatttgt taaatatgta ctacaaactt agtagt //