LOCUS       V018619                10036 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018619
VERSION     V018619
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2809..4719
                     /label="Prmt5(NM_001108867)"
                     /note="Prmt5(NM_001108867)"
                     /gene="Prmt5"
     misc_feature    4739..5325
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             5345..5944
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5958..6546
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6753..7386
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7514..7530)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7538..7554
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7562..7592)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7607..7628
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7916..8504)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8675..9535)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9536..9640)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9688..9822
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcat ggcggcgatg
     2821 gcagtcggag gtgctggtgg cagccgcgtg tccagcggga gggacctgaa ttgcgtccct
     2881 gaaatagctg acacactggg tgctgtggcc aagcaggggt ttgatttcct ctgcatgcct
     2941 gtcttccacc cgcgtttcaa gagggagttc attcaggaac ctgctaagaa tcggcctggc
     3001 ccccagacac gatcagacct actgctgtca ggaagggact ggaatacgct aattgtggga
     3061 aagctttctc catggattca tccagactca aaagtggaga agatccgaag gaactctgag
     3121 gcggctatgt tgcaggagct gaattttggg gcatatctag gtcttccagc tttcctattg
     3181 ccccttaatc aggaagataa cacaaatctg gccagagttc tgaccaacca catccacact
     3241 ggccaccatt cttccatgtt ctggatgagg gtccccttgg tggccccaga ggacctgaga
     3301 gatgatgtaa ttgagaatgc accaactgca cacacagagg agtacagtgg agaggagaag
     3361 acgtggttgt ggtggcataa cttccggact ctgtgtgact atagtaagag gattgcagta
     3421 gctcttgaaa tcggagctga cctcccgtct agtcacgtca ttgatcgttg gcttggagag
     3481 cccatcaaag ccgccattct ccccaccagc attttcctaa ccaacaagaa ggggtttcct
     3541 gttctttcta agatgcagca gaggctgatc ttccggctcc tcaagttgga agtgcaattt
     3601 atcatcacgg gaaccaacca ccactcagag aaggagttct gctcctacct ccagtacttg
     3661 gaatacttaa gccaaaatcg tcccccaccc aatgcttacg aactctttgc caaaggctat
     3721 gaagactatc tgcagtcccc acttcagcct ctgatggaca atctggaatc tcagacgtac
     3781 gaagtgttcg aaaaggaccc catcaaatac tctcagtacc agcaggccat ctataaatgt
     3841 ttgctagacc gagtaccaga agaagaaaag gagaccaatg tccaggtact tatggtgctg
     3901 ggtgcaggcc gaggtcctct agtgaatgcg tctctccggg cagccaagca ggctgagcgg
     3961 cggattaggc tgtatgccgt ggagaagaac cccaatgctg tggtgacgct agagaactgg
     4021 cagtttgaag aatgggggag tcaggtgaca gttgtctcgt cagacatgcg ggaatgggtg
     4081 gctccggaga aggcagacat cattgtcagt gaacttctgg gctcctttgc cgacaatgag
     4141 ctgtcacctg agtgtctgga tggagcacag cacttcctga aagatgatgg tgtaagcatc
     4201 cccggagaat acacctcctt cctggctccc atttcttcct cgaagttgta caacgaggtc
     4261 cgcgcctgtc gggagaagga ccgtgaccct gaggcacagt ttgagatgcc ttatgtggtt
     4321 cggctgcaca acttccacca gctttctgct cctcagccct gttttacctt cagccatccc
     4381 aaccgagatc ctatgattga caacaaccgc tactgtacct tggagtttcc tgtggaggtg
     4441 aacacggtgc ttcatggctt tgcaggctac tttgaaactg tgctttaccg ggacatcact
     4501 ctgagtatcc gcccagagac ccactctcct ggcatgttct catggttccc catcttcttc
     4561 cccatcaagc agcccatcac ggtgcatgaa ggccagaaca tctgtgtgcg tttctggcga
     4621 tgcagcaatt ctaagaaggt gtggtacgag tgggcggtga cagcgccagt ctgttcttct
     4681 attcacaacc ctactggtcg ctcctatacc attggcctct aggcggccgc ggatcccgcc
     4741 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     4801 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     4861 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     4921 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     4981 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     5041 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     5101 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     5161 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     5221 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     5281 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccacaa ggagacgacc
     5341 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     5401 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     5461 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     5521 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     5581 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     5641 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     5701 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     5761 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     5821 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     5881 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     5941 ctagacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     6001 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6061 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6121 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6181 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6241 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6301 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     6361 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     6421 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6481 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6541 ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag
     6601 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     6661 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     6721 tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag
     6781 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
     6841 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     6901 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     6961 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
     7021 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
     7081 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
     7141 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     7201 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7261 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7321 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7381 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7441 tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct agctagagct
     7501 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7561 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7621 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7681 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7741 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7801 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7861 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7921 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7981 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8041 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8101 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8161 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8221 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8281 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8341 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8401 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8461 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8521 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8581 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8641 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8701 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8761 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8821 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8881 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8941 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9001 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9061 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9121 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9181 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9241 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9301 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9361 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9421 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9481 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9541 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9601 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9661 cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa
     9721 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9781 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
     9841 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
     9901 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
     9961 tgttaaatat gtactacaaa cttagtagtt tttaaagaaa ttgtatttgt taaatatgta
    10021 ctacaaactt agtagt
//
