LOCUS       V026348                 9897 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026348
VERSION     V026348
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..4527
                     /label="OTUD5(NM_017602)"
                     /note="OTUD5(NM_017602)"
                     /gene="OTUD5"
     CDS             4534..4563
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     misc_feature    4600..5186
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             5206..5805
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5819..6407
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6614..7247
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(7375..7391)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7399..7415
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7423..7453)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7468..7489
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7777..8365)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8536..9396)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9397..9501)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    9549..9683
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgact
     2821 atactcccca aaaagaagcc gccgcctccc gacgccgacc ccgccaacga gccgccgccg
     2881 cccgggccga tgcccccggc gccgcggcgc ggcggaggtg tgggcgtggg cggcggcggc
     2941 acgggcgtgg gcggcggcga tcgcgaccgt gactccggcg tcgtgggggc ccgtccgcga
     3001 gcttcgccac cgcctcaagg cccgctacca ggaccgccgg gcgctcttca tcgctgggcg
     3061 ctggccgtgc cgcctggtgc agtggcgggt ccccggccac aacaggcttc tccacctcct
     3121 tgcgggggcc caggtggtcc cggcggcggt cccggcgacg cgctgggcgc agcggcggcg
     3181 ggtgtgggtg ccgcgggcgt ggtggtgggt gtgggtggtg ccgtaggcgt gggcggctgc
     3241 tgctccgggc ctgggcacag caagcggcga cgtcaagctc ccggggttgg cgcggttggc
     3301 gggggcagtc ccgagcgtga ggaggtcggc gcaggctaca acagtgagga cgagtatgag
     3361 gcggctgcag cacgcatcga ggctatggac cctgccactg tcgagcagca ggagcattgg
     3421 tttgaaaagg ccctacgaga caagaagggc ttcatcatca agcagatgaa ggaggatggc
     3481 gcctgtctct tccgggctgt agctgaccag gtgtatggag accaggacat gcatgaggtt
     3541 gtgcgaaagc attgcatgga ctatctgatg aagaatgccg actacttctc caactatgtc
     3601 acagaggact ttaccaccta cattaacagg aagcggaaaa acaattgcca tggcaaccac
     3661 attgagatgc aggccatggc agagatgtac aaccgtcctg tggaggtgta ccagtacagc
     3721 acaggtactt ctgcagtgga acccatcaac acattccatg ggatacatca aaacgaggac
     3781 gaacccattc gtgttagcta ccatcggaat atccactata attcagtggt gaatcctaac
     3841 aaggccacca ttggtgtggg gctgggcctg ccatcattca aaccagggtt tgcagagcag
     3901 tctctgatga agaatgccat aaaaacatcg gaggagtcat ggattgaaca gcagatgcta
     3961 gaagacaaga aacgggccac agactgggag gccacaaatg aagccatcga ggagcaggtg
     4021 gctcgggaat cctacctgca gtggttgcgg gatcaggaga aacaggctcg ccaggtccga
     4081 ggccccagcc agccccggaa agccagcgcc acatgcagtt cggccacagc agcagcctcc
     4141 agtggcctgg aggagtggac tagccggtcc ccgcggcagc ggagttcagc ctcgtcacct
     4201 gagcaccctg agctgcatgc tgaattgggc atgaagcccc cttccccagg cactgtttta
     4261 gctcttgcca aacctccttc gccctgtgcg ccaggtacaa gcagtcagtt ctcggcaggg
     4321 gccgaccggg caacttcccc ccttgtgtcc ctctaccctg ctttggagtg ccgggccctc
     4381 attcagcaga tgtccccctc tgcctttggt ctgaatgact gggatgatga tgagatccta
     4441 gcttcggtgc tggcagtgtc ccaacaggaa tacctagaca gtatgaagaa aaacaaagtg
     4501 cacagagacc cgcccccaga caagagtctc gaggaacaaa aactcatctc agaagaggat
     4561 ctgtgatcga gactagttct agagcggccg cggatcccgc ccctctccct cccccccccc
     4621 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     4681 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     4741 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     4801 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     4861 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     4921 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     4981 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     5041 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     5101 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     5161 aacacgatga taagcttgcc acaacccaca aggagacgac cttccatgac cgagtacaag
     5221 cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc
     5281 gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg
     5341 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5401 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5461 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     5521 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     5581 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     5641 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     5701 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     5761 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctagacgcg tctggaacaa
     5821 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5881 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5941 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6001 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6061 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6121 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6181 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     6241 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6301 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6361 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc
     6421 tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     6481 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     6541 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6601 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     6661 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     6721 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     6781 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     6841 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     6901 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     6961 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     7021 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     7081 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7141 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7201 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7261 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     7321 ttgacattgc tagcgtttac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7381 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7441 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7501 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7561 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7621 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7681 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7741 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7801 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7861 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7921 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7981 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8041 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8101 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8161 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8221 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8281 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8341 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8401 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8461 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8521 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8581 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8641 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8701 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8761 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8821 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8881 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8941 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9001 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9061 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9121 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9181 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9241 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9301 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9361 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9421 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9481 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     9541 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9601 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9661 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     9721 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     9781 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     9841 acttagtagt ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt
//
