LOCUS       V044313                12154 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044313
VERSION     V044313
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2821..5802
                     /label="Mertk(NM_008587)"
                     /note="Mertk(NM_008587)"
                     /gene="Mertk"
     CDS             5803..5826
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             5827..5850
                     /codon_start=1
                     /product="recognition and cleavage site for human
                     rhinovirus 3C and PreScission proteases"
                     /transl_table=1
                     /translation="LEVLFQGP"
                     /label="HRV 3C site"
     CDS             5851..6807
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID
                     engineered for improved catalytic efficiency (Branon et
                     al., 2018)"
                     /transl_table=1
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
                     /label="TurboID"
     misc_feature    6857..7443
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             7463..8062
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8076..8664
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8871..9504
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9632..9648)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9656..9672
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9680..9710)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9725..9746
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10034..10622)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10793..11653)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11654..11758)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11806..11940
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattact cgaggccacc
     2821 atggttctgg ccccactgct actggggctg ctgctgctac ccgcgctctg gagtggaggc
     2881 actgccgaga agtgggaaga gaccgagcta gatcagctat tttcagggcc tttaccaggg
     2941 agactcccag tcaaccacag gccattctct gctcctcact ccagccggga ccagctgcca
     3001 ccaccccaga ctggaagatc acatccagca cacacagccg ctccccaggt gacctccaca
     3061 gcatcaaagc tcctacctcc tgttgcgttt aatcacacca ttggacacat agtactgtcg
     3121 gaacataaaa atgtcaaatt taattgctcc atcaatattc ctaacacata ccaagaaaca
     3181 gctggcattt catggtggaa agatggaaag gaattgctcg gggcacatca ttcaatcaca
     3241 cagttttatc ctgatgagga aggggtatca ataattgcat tgttcagcat agccagtgtg
     3301 cagcgctcag acaatgggtc gtacttctgt aagatgaagg tgaacaatag agagattgta
     3361 tctgatccca tatacgtgga agttcaagga ctcccttact ttattaagca gcctgagagt
     3421 gtgaatgtca ccagaaacac agccttcaac ctcacctgcc aggccgtggg ccctcctgag
     3481 cccgtcaata tcttctgggt tcaaaatagc agccgtgtta atgaaaaacc ggaaaggtcc
     3541 ccgtctgtcc taaccgtacc tggtctgaca gagacagcag tcttcagctg tgaggcccac
     3601 aatgacaaag gactgacggt gtccaagggt gtacatatca acatcaaagt aatcccctcc
     3661 ccgcccactg aagtccatat cctcaacagt acagcacaca gcatcctggt ctcctgggtc
     3721 cctggttttg atggctactc cccacttcag aactgcagca ttcaggtcaa ggaagctgac
     3781 cggctgagta atggctcagt catggttttt aatacctctg cttcgccaca tctgtatgag
     3841 atccagcagc tgcaagccct ggctaattac agcatcgctg tgtcctgtcg gaatgagatt
     3901 ggctggtctg cagtaagccc ttggattctg gccagcacaa cagaaggagc tccatctgta
     3961 gcacctttaa acatcactgt gtttctgaac gaatctaaca atatcctgga tattagatgg
     4021 acgaagcctc caattaagcg gcaggatggg gaactggtgg gctaccggat atctcacgtg
     4081 tgggaaagcg cagggactta caaagagctt tctgaagaag tcagccagaa tggcagctgg
     4141 gctcagattc ctgtccaaat ccacaatgcc acctgcacag tgagaatcgc ggccattact
     4201 aaagggggca tcgggccctt cagtgagcca gtgaatatca tcattcctga acacagtaag
     4261 gtagattacg caccctcgtc aaccccagcc cctggcaaca ccgactctat gttcatcatc
     4321 ctcggctgct tctgtggatt cattttaatc gggttaattt tgtgtatttc tctggccctc
     4381 agaaggagag tccaggaaac aaagtttggg ggagcattct ctgaggagga ttcccaactg
     4441 gtcgtaaatt atagagcgaa gaagtccttc tgccggcgag ccatcgagct taccttgcag
     4501 agcctgggag tgagcgagga gctgcagaat aagctggaag atgttgtgat tgacagaaac
     4561 cttctggttc tcggcaaagt tctgggtgaa ggagagtttg ggtctgtaat ggaaggaaat
     4621 ttgaagcaag aagatgggac ttctcagaag gtggcagtga agaccatgaa gttggacaac
     4681 ttttctcaac gggagatcga ggagtttctc agcgaagcag catgcatgaa agacttcaac
     4741 cacccaaatg tcatccgact tctaggcgtg tgtatagaac tgagctctca aggcatcccg
     4801 aagcccatgg tgattttacc cttcatgaaa tacggagacc tccacacctt cctgttatat
     4861 tcccgattaa acacaggacc caagtacatt cacctgcaga cactactgaa gttcatgatg
     4921 gacattgccc agggaatgga gtatctgagc aacaggaatt ttcttcatag ggatttggca
     4981 gctcgaaact gcatgttgcg ggatgacatg actgtctgcg tggcagactt tggcctctca
     5041 aagaagattt acagtggtga ttattaccgc caaggccgca ttgccaaaat gcctgtgaag
     5101 tggatcgcca tcgagagcct ggcggaccga gtctacacaa gcaaaagtga cgtgtgggct
     5161 tttggcgtga ccatgtggga aataacaaca cggggaatga ctccctatcc cggagttcag
     5221 aaccatgaga tgtacgacta ccttctccac ggccacaggc tgaagcagcc tgaggactgc
     5281 ttggatgaac tgtatgacat catgtactct tgctggagtg ctgatccctt ggatcgaccc
     5341 accttctctg tgttgaggct gcagctggaa aagctctccg agagtttgcc tgatgcgcag
     5401 gacaaagaat ccatcatcta catcaatacc cagttgctag agagctgcga gggcatagcc
     5461 aatgggccct cactcacggg gctagacatg aacattgacc ctgactccat cattgcctct
     5521 tgcacaccag gcgctgccgt cagcgtggtc acggcagaag ttcacgagaa caaccttcgt
     5581 gaggaaagat acatcttgaa tgggggcaat gaggaatggg aagatgtgtc ctccactcct
     5641 tttgctgcag tcacacctga aaaggatggt gtcttaccgg aggacagact caccaaaaat
     5701 ggcgtctcct ggtctcacca tagtacacta cccttgggga gcccatcacc agatgaactt
     5761 ttatttgtag atgactcctt ggaagactct gaagttctga tggattataa agatgatgat
     5821 gataaactgg aagttctgtt ccaggggccc aaagacaata ctgtgcctct gaagctgatc
     5881 gctctcctgg ctaatggcga gttccatagt ggcgaacagc tgggagaaac cctgggcatg
     5941 tccagggccg ctatcaacaa gcacattcag actctgcgcg actggggcgt ggacgtgttc
     6001 accgtgcccg gaaagggcta ctctctgccc gagcctatcc cgctgctgaa cgctaaacag
     6061 attctgggac agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga ctccaccaat
     6121 cagtacctgc tggatcgaat cggcgagctg aagagtgggg atgcttgcat tgcagaatat
     6181 cagcaggcag ggagaggaag cagagggagg aaatggttct ctccttttgg agctaacctg
     6241 tacctgagta tgttttggcg cctgaagcgg ggaccagcag caatcggcct gggcccggtc
     6301 atcggaattg tcatggcaga agcgctgcga aagctgggag cagacaaggt gcgagtcaaa
     6361 tggcccaatg acctgtatct gcaggataga aagctggcag gcatcctggt ggagctggcc
     6421 ggaataacag gcgatgctgc acagatcgtc attggcgccg ggattaacgt ggctatgagg
     6481 cgcgtggagg aaagcgtggt caatcagggc tggatcacac tgcaggaagc agggattaac
     6541 ctggacagga atactctggc cgctacgctg atccgagagc tgcgggcagc cctggaactg
     6601 ttcgagcagg aaggcctggc tccatatctg ccacggtggg agaagctgga taacttcatc
     6661 aatagacccg tgaagctgat cattggggac aaagagattt tcgggattag ccgggggatt
     6721 gataaacagg gagccctgct gctggaacag gacggagtta tcaaaccctg gatgggcgga
     6781 gaaatcagtc tgcggtctgc cgaaaagtag gaattcgcca attcgctgga gcagactaga
     6841 gcggccgcgg atcccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     6901 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     6961 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     7021 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     7081 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     7141 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     7201 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     7261 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     7321 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     7381 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccaca
     7441 acccacaagg agacgacctt ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     7501 cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     7561 gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     7621 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     7681 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     7741 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     7801 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     7861 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     7921 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     7981 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     8041 gacccgcaag cccggtgcct agacgcgtct ggaacaatca acctctggat tacaaaattt
     8101 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     8161 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     8221 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     8281 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     8341 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     8401 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     8461 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     8521 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     8581 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     8641 tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca
     8701 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     8761 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     8821 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct
     8881 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     8941 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     9001 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     9061 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     9121 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     9181 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     9241 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     9301 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     9361 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     9421 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     9481 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     9541 aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag cgtttaccgt
     9601 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     9661 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9721 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9781 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     9841 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9901 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9961 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    10021 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    10081 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    10141 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    10201 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    10261 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10321 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10381 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10441 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    10501 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10561 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10621 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10681 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10741 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10801 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10861 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10921 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10981 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    11041 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    11101 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    11161 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    11221 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    11281 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    11341 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    11401 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    11461 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    11521 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11581 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11641 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11701 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    11761 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca
    11821 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    11881 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    11941 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
    12001 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
    12061 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt
    12121 gtatttgtta aatatgtact acaaacttag tagt
//
