LOCUS V044313 12154 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V044313 VERSION V044313 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" enhancer 2201..2504 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 2505..2708 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2821..5802 /label="Mertk(NM_008587)" /note="Mertk(NM_008587)" /gene="Mertk" CDS 5803..5826 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" CDS 5827..5850 /codon_start=1 /product="recognition and cleavage site for human rhinovirus 3C and PreScission proteases" /transl_table=1 /translation="LEVLFQGP" /label="HRV 3C site" CDS 5851..6807 /codon_start=1 /product="mutant of the E. coli biotin ligase BioID engineered for improved catalytic efficiency (Branon et al., 2018)" /transl_table=1 /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK" /label="TurboID" misc_feature 6857..7443 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES2" CDS 7463..8062 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 8076..8664 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 8871..9504 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(9632..9648) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 9656..9672 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(9680..9710) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 9725..9746 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(10034..10622) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(10793..11653) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(11654..11758) /gene="bla" /label="AmpR promoter" polyA_signal 11806..11940 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattact cgaggccacc 2821 atggttctgg ccccactgct actggggctg ctgctgctac ccgcgctctg gagtggaggc 2881 actgccgaga agtgggaaga gaccgagcta gatcagctat tttcagggcc tttaccaggg 2941 agactcccag tcaaccacag gccattctct gctcctcact ccagccggga ccagctgcca 3001 ccaccccaga ctggaagatc acatccagca cacacagccg ctccccaggt gacctccaca 3061 gcatcaaagc tcctacctcc tgttgcgttt aatcacacca ttggacacat agtactgtcg 3121 gaacataaaa atgtcaaatt taattgctcc atcaatattc ctaacacata ccaagaaaca 3181 gctggcattt catggtggaa agatggaaag gaattgctcg gggcacatca ttcaatcaca 3241 cagttttatc ctgatgagga aggggtatca ataattgcat tgttcagcat agccagtgtg 3301 cagcgctcag acaatgggtc gtacttctgt aagatgaagg tgaacaatag agagattgta 3361 tctgatccca tatacgtgga agttcaagga ctcccttact ttattaagca gcctgagagt 3421 gtgaatgtca ccagaaacac agccttcaac ctcacctgcc aggccgtggg ccctcctgag 3481 cccgtcaata tcttctgggt tcaaaatagc agccgtgtta atgaaaaacc ggaaaggtcc 3541 ccgtctgtcc taaccgtacc tggtctgaca gagacagcag tcttcagctg tgaggcccac 3601 aatgacaaag gactgacggt gtccaagggt gtacatatca acatcaaagt aatcccctcc 3661 ccgcccactg aagtccatat cctcaacagt acagcacaca gcatcctggt ctcctgggtc 3721 cctggttttg atggctactc cccacttcag aactgcagca ttcaggtcaa ggaagctgac 3781 cggctgagta atggctcagt catggttttt aatacctctg cttcgccaca tctgtatgag 3841 atccagcagc tgcaagccct ggctaattac agcatcgctg tgtcctgtcg gaatgagatt 3901 ggctggtctg cagtaagccc ttggattctg gccagcacaa cagaaggagc tccatctgta 3961 gcacctttaa acatcactgt gtttctgaac gaatctaaca atatcctgga tattagatgg 4021 acgaagcctc caattaagcg gcaggatggg gaactggtgg gctaccggat atctcacgtg 4081 tgggaaagcg cagggactta caaagagctt tctgaagaag tcagccagaa tggcagctgg 4141 gctcagattc ctgtccaaat ccacaatgcc acctgcacag tgagaatcgc ggccattact 4201 aaagggggca tcgggccctt cagtgagcca gtgaatatca tcattcctga acacagtaag 4261 gtagattacg caccctcgtc aaccccagcc cctggcaaca ccgactctat gttcatcatc 4321 ctcggctgct tctgtggatt cattttaatc gggttaattt tgtgtatttc tctggccctc 4381 agaaggagag tccaggaaac aaagtttggg ggagcattct ctgaggagga ttcccaactg 4441 gtcgtaaatt atagagcgaa gaagtccttc tgccggcgag ccatcgagct taccttgcag 4501 agcctgggag tgagcgagga gctgcagaat aagctggaag atgttgtgat tgacagaaac 4561 cttctggttc tcggcaaagt tctgggtgaa ggagagtttg ggtctgtaat ggaaggaaat 4621 ttgaagcaag aagatgggac ttctcagaag gtggcagtga agaccatgaa gttggacaac 4681 ttttctcaac gggagatcga ggagtttctc agcgaagcag catgcatgaa agacttcaac 4741 cacccaaatg tcatccgact tctaggcgtg tgtatagaac tgagctctca aggcatcccg 4801 aagcccatgg tgattttacc cttcatgaaa tacggagacc tccacacctt cctgttatat 4861 tcccgattaa acacaggacc caagtacatt cacctgcaga cactactgaa gttcatgatg 4921 gacattgccc agggaatgga gtatctgagc aacaggaatt ttcttcatag ggatttggca 4981 gctcgaaact gcatgttgcg ggatgacatg actgtctgcg tggcagactt tggcctctca 5041 aagaagattt acagtggtga ttattaccgc caaggccgca ttgccaaaat gcctgtgaag 5101 tggatcgcca tcgagagcct ggcggaccga gtctacacaa gcaaaagtga cgtgtgggct 5161 tttggcgtga ccatgtggga aataacaaca cggggaatga ctccctatcc cggagttcag 5221 aaccatgaga tgtacgacta ccttctccac ggccacaggc tgaagcagcc tgaggactgc 5281 ttggatgaac tgtatgacat catgtactct tgctggagtg ctgatccctt ggatcgaccc 5341 accttctctg tgttgaggct gcagctggaa aagctctccg agagtttgcc tgatgcgcag 5401 gacaaagaat ccatcatcta catcaatacc cagttgctag agagctgcga gggcatagcc 5461 aatgggccct cactcacggg gctagacatg aacattgacc ctgactccat cattgcctct 5521 tgcacaccag gcgctgccgt cagcgtggtc acggcagaag ttcacgagaa caaccttcgt 5581 gaggaaagat acatcttgaa tgggggcaat gaggaatggg aagatgtgtc ctccactcct 5641 tttgctgcag tcacacctga aaaggatggt gtcttaccgg aggacagact caccaaaaat 5701 ggcgtctcct ggtctcacca tagtacacta cccttgggga gcccatcacc agatgaactt 5761 ttatttgtag atgactcctt ggaagactct gaagttctga tggattataa agatgatgat 5821 gataaactgg aagttctgtt ccaggggccc aaagacaata ctgtgcctct gaagctgatc 5881 gctctcctgg ctaatggcga gttccatagt ggcgaacagc tgggagaaac cctgggcatg 5941 tccagggccg ctatcaacaa gcacattcag actctgcgcg actggggcgt ggacgtgttc 6001 accgtgcccg gaaagggcta ctctctgccc gagcctatcc cgctgctgaa cgctaaacag 6061 attctgggac agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga ctccaccaat 6121 cagtacctgc tggatcgaat cggcgagctg aagagtgggg atgcttgcat tgcagaatat 6181 cagcaggcag ggagaggaag cagagggagg aaatggttct ctccttttgg agctaacctg 6241 tacctgagta tgttttggcg cctgaagcgg ggaccagcag caatcggcct gggcccggtc 6301 atcggaattg tcatggcaga agcgctgcga aagctgggag cagacaaggt gcgagtcaaa 6361 tggcccaatg acctgtatct gcaggataga aagctggcag gcatcctggt ggagctggcc 6421 ggaataacag gcgatgctgc acagatcgtc attggcgccg ggattaacgt ggctatgagg 6481 cgcgtggagg aaagcgtggt caatcagggc tggatcacac tgcaggaagc agggattaac 6541 ctggacagga atactctggc cgctacgctg atccgagagc tgcgggcagc cctggaactg 6601 ttcgagcagg aaggcctggc tccatatctg ccacggtggg agaagctgga taacttcatc 6661 aatagacccg tgaagctgat cattggggac aaagagattt tcgggattag ccgggggatt 6721 gataaacagg gagccctgct gctggaacag gacggagtta tcaaaccctg gatgggcgga 6781 gaaatcagtc tgcggtctgc cgaaaagtag gaattcgcca attcgctgga gcagactaga 6841 gcggccgcgg atcccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct 6901 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg 6961 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt 7021 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg 7081 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac 7141 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg 7201 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct 7261 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg 7321 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag 7381 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccaca 7441 acccacaagg agacgacctt ccatgaccga gtacaagccc acggtgcgcc tcgccacccg 7501 cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac 7561 gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt 7621 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt 7681 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc 7741 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct 7801 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga 7861 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg 7921 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg 7981 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat 8041 gacccgcaag cccggtgcct agacgcgtct ggaacaatca acctctggat tacaaaattt 8101 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 8161 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 8221 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 8281 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 8341 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 8401 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 8461 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc 8521 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 8581 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 8641 tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca 8701 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc 8761 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac 8821 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct 8881 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta 8941 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg 9001 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga 9061 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt 9121 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc 9181 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt 9241 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata 9301 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc 9361 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 9421 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 9481 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat 9541 aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag cgtttaccgt 9601 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 9661 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 9721 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 9781 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 9841 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 9901 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 9961 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 10021 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 10081 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 10141 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 10201 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 10261 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 10321 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 10381 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 10441 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 10501 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 10561 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 10621 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 10681 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 10741 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 10801 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 10861 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 10921 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 10981 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 11041 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 11101 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 11161 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 11221 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 11281 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 11341 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 11401 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 11461 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 11521 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 11581 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 11641 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 11701 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 11761 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca 11821 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 11881 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 11941 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc 12001 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca 12061 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt 12121 gtatttgtta aatatgtact acaaacttag tagt //