LOCUS       V045733                10654 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045733
VERSION     V045733
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..5298
                     /label="GPAM(NM_001244949)"
                     /note="GPAM(NM_001244949)"
                     /gene="GPAM"
     CDS             5299..5325
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    5357..5943
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             5963..6562
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6576..7164
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7371..8004
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(8132..8148)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8156..8172
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8180..8210)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8225..8246
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8534..9122)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9293..10153)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10154..10258)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    10306..10440
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggat
     2821 gaatctgcac tgacccttgg tacaatagat gtttcttatc tgccacattc atcagaatac
     2881 agtgttggtc gatgtaagca cacaagtgag gaatggggtg agtgtggctt tagacccacc
     2941 atcttcagat ctgcaacttt aaaatggaaa gaaagcctaa tgagtcggaa aaggccattt
     3001 gttggaagat gttgttactc ctgcactccc cagagctggg acaaattttt caaccccagt
     3061 atcccgtctt tgggtttgcg gaatgttatt tatatcaatg aaactcacac aagacaccgc
     3121 ggatggcttg caagacgcct ttcttacgtt ctttttattc aagagcgaga tgtgcataag
     3181 ggcatgtttg ccaccaatgt gactgaaaat gtgctgaaca gcagtagagt acaagaggca
     3241 attgcagaag tggctgctga attaaaccct gatggttctg cccagcagca atcaaaagcc
     3301 gttaacaaag tgaaaaagaa agctaaaagg attcttcaag aaatggttgc cactgtctca
     3361 ccggcaatga tcagactgac tgggtgggtg ctgctaaaac tgttcaacag cttcttttgg
     3421 aacattcaaa ttcacaaagg tcaacttgag atggttaaag ctgcaactga gacgaatttg
     3481 ccgcttctgt ttctaccagt tcatagatcc catattgact atctgctgct cactttcatt
     3541 ctcttctgcc ataacatcaa agcaccatac attgcttcag gcaataatct caacatccca
     3601 atcttcagta ccttgatcca taagcttggg ggcttcttca tacgacgaag gctcgatgaa
     3661 acaccagatg gacggaaaga tgttctctat agagctttgc tccatgggca tatagttgaa
     3721 ttacttcgac agcagcaatt cttggagatc ttcctggaag gcacacgttc taggagtgga
     3781 aaaacctctt gtgctcgggc aggacttttg tcagttgtgg tagatactct gtctaccaat
     3841 gtcatcccag acatcttgat aatacctgtt ggaatctcct atgatcgcat tatcgaaggt
     3901 cactacaatg gtgaacaact gggcaaacct aagaagaatg agagcctgtg gagtgtagca
     3961 agaggtgtta ttagaatgtt acgaaaaaac tatggttgtg tccgagtgga ttttgcacag
     4021 ccattttcct taaaggaata tttagaaagc caaagtcaga aaccggtgtc tgctctactt
     4081 tccctggagc aagcgttgtt accagctata cttccttcaa gacccagtga tgctgctgat
     4141 gaaggtagag acacgtccat taatgagtcc agaaatgcaa cagatgaatc cctacgaagg
     4201 aggttgattg caaatctggc tgagcatatt ctattcactg ctagcaagtc ctgtgccatt
     4261 atgtccacac acattgtggc ttgcctgctc ctctacagac acaggcaggg aattgatctc
     4321 tccacattgg tcgaagactt ctttgtgatg aaagaggaag tcctggctcg tgattttgac
     4381 ctggggttct caggaaattc agaagatgta gtaatgcatg ccatacagct gctgggaaat
     4441 tgtgtcacaa tcacccacac tagcaggaac gatgagtttt ttatcacccc cagcacaact
     4501 gtcccatcag tcttcgaact caacttctac agcaatgggg tacttcatgt ctttatcatg
     4561 gaggccatca tagcttgcag cctttatgca gttctgaaca agaggggact ggggggtccc
     4621 actagcaccc cacctaacct gatcagccag gagcagctgg tgcggaaggc ggccagcctg
     4681 tgctaccttc tctccaatga aggcaccatc tcactgcctt gccagacatt ttaccaagtc
     4741 tgccatgaaa cagtaggaaa gtttatccag tatggcattc ttacagtggc agagcacgat
     4801 gaccaggaag atatcagtcc tagtcttgct gagcagcagt gggacaagaa gcttccagaa
     4861 cctttgtctt ggagaagtga tgaagaagat gaagacagtg actttgggga ggaacagcga
     4921 gattgctacc tgaaggtgag ccaatccaag gagcaccagc agtttatcac cttcttacag
     4981 agactccttg ggcctttgct ggaggcctac agctctgctg ccatctttgt tcacaacttc
     5041 agtggtcctg ttccagaacc tgagtatctg caaaagttgc acaaatacct aataaccaga
     5101 acagaaagaa atgttgcagt atatgctgag agtgccacat attgtcttgt gaagaatgct
     5161 gtgaaaatgt ttaaggatat tggggttttc aaggagacca aacaaaagag agtgtctgtt
     5221 ttagaactga gcagcacttt tctacctcaa tgcaaccgac aaaaacttct agaatatatt
     5281 ctgagttttg tggtgctgta cccatacgat gttccagatt acgcttagac tagttctaga
     5341 gcggccgcgg atcccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     5401 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     5461 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     5521 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     5581 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     5641 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     5701 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     5761 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     5821 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     5881 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccaca
     5941 acccacaagg agacgacctt ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     6001 cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     6061 gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     6121 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     6181 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     6241 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     6301 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     6361 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     6421 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     6481 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     6541 gacccgcaag cccggtgcct agacgcgtct ggaacaatca acctctggat tacaaaattt
     6601 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6661 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6721 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6781 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6841 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6901 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6961 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     7021 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     7081 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     7141 tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca
     7201 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     7261 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     7321 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct
     7381 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     7441 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     7501 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     7561 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     7621 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     7681 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     7741 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     7801 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7861 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7921 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7981 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     8041 aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag cgtttaccgt
     8101 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8161 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8221 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8281 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8341 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8401 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8461 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8521 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8581 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8641 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8701 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8761 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8821 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8881 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8941 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9001 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9061 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9121 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9181 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9241 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9301 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9361 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9421 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9481 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9541 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9601 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9661 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9721 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9781 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9841 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9901 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9961 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10021 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10081 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10141 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10201 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10261 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca
    10321 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    10381 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    10441 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
    10501 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
    10561 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt
    10621 gtatttgtta aatatgtact acaaacttag tagt
//
