LOCUS       V033870                12226 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033870
VERSION     V033870
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2505..2708
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2815..6846
                     /label="Eif3a(NM_010123)"
                     /note="Eif3a(NM_010123)"
                     /gene="Eif3a"
     CDS             6853..6918
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    6929..7515
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES2"
     CDS             7535..8134
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8148..8736
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8943..9576
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9704..9720)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9728..9744
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9752..9782)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9797..9818
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10106..10694)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10865..11725)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11726..11830)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11878..12012
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgccg
     2821 gcctatttcc agaggccgga aaatgccctc aagcgcgcca acgaatttct tgaggttggc
     2881 aaaaagcagc ctgccctgga tgttctttat gatgtaatga aaagtaagaa gcacagaaca
     2941 tggcagaaga tacatgagcc aattatgctg aaatacttgg aactgtgtgt ggatcttcgt
     3001 aagagccact tggctaagga agggttatat caatataaga acatatgcca acaggtaaac
     3061 attaaatctt tagaagatgt tgttagggca tatttgaaat tagcagagga aaaaacagaa
     3121 gctgctaaag aagagtccca acaaatggtg ttagatatag aagatctgga taatattcag
     3181 actcctgaga gtgttctctt aagtgcagta agtggggaag atactcagga tcgtactgac
     3241 agattgctac tgactccctg ggtcaagttc ctgtgggaat catacaggca gtgtttggac
     3301 cttcttcgaa acaattctag agtagagcgc ctttaccatg atatcgccca acaagctttc
     3361 aaattctgcc tccagtatac tcggaaggct gagttccgca agctatgtga caacttgcga
     3421 atgcacttat cccagattca gcgccaccat aaccaaagca cagcaattaa tcttaataat
     3481 ccagagagcc agtctatgca tttggaaacc agacttgttc agttggacag tgctatcagc
     3541 atggaattat ggcaggaagc cttcaaagct gtggaagata ttcatggact attttccttg
     3601 tctaagaaac cacctaagcc tcagttgatg gcaaattact ataacaaagt ttcaacagtg
     3661 ttttggaaat ctggaaatgc tttgttccat gcatctacac ttcatcgtct ttatcatctg
     3721 tctagagaaa tgagaaagaa tcttacacaa gaagagatgc aaagaatgtc tactagagtc
     3781 cttttggcga ctctttccat tcctattact cctgagcgaa ctgatattgc tcgacttttg
     3841 gatatggatg gtattatagt tgagaagcag cgacgccttg ccacattgct aggtcttcaa
     3901 gccccaccca cacgaatcgg cctaattaat gatatggtca ggttcagtgt gctacagtat
     3961 gttgtcccag aagtgaaaga cctttacaac tggttggagg tggaattcaa cccactaaaa
     4021 ctctgtgaga gagttacaaa ggtattaaat tgggttaggg aacaacctga aaaagaacca
     4081 gaattgcaac aatatgtacc acaactccag aacaatacca tactccgcct tctgcaacag
     4141 gtggcacaga tttatcagag cattgagttt tctcgtttga cttctctggt tccttttgtt
     4201 gatgctttcc aactggaacg ggccatagta gatgcagcca ggcactgtga cctgcaggta
     4261 cgtattgacc atacttcccg gactctgagt tttggatcag atttgaatta tgcaactcga
     4321 gaagatgccc cagttggccc tcatctgcag agcatgcctt cagagcagat aagaaaccag
     4381 ctcacggcca tgtcctcagt gcttgccaaa gcacttgaag tcatcagacc tgctcacatt
     4441 ctgcaagaga aagaagaaca gcatcaattg gcagttaatg catatcttaa aaattcaaga
     4501 aaagagcacc agaggatcct ggctcggaga cagacaattg aggaaagaaa agagcgtctt
     4561 gagagtctga atattcaacg tgagaaggaa gaactcgagc agagggaagc tgaactccag
     4621 aaagtacgaa aggctgaaga agaaaggctg cgccaagagg caaaggagcg agagaaggaa
     4681 cgaatccttc aagaacacga gcaaatcaag aagaaaactg ttcgggagcg gttagagcag
     4741 atcaagaaga cagagctggg cgccaaagca tttaaagata ttgacattga agaccttgaa
     4801 gaactggatc cagattttat tatggccaaa caggttgaac aactggagaa agaaaagaag
     4861 gaacttcagg aacgcctgaa gaatcaagaa aagaagattg actattttga gagagctaag
     4921 cgtttggaag aaattccttt aataaagagt gcttatgagg aacaaagggt taaagacatg
     4981 gacctgtggg aacagcaaga agaagaaaga atcactacaa tgcagctaga acgagaaaaa
     5041 gctctggagc ataagaatag gatgtcacga atgttggaag acagagatct atttgtgatg
     5101 cgcctcaaag ctgcccggca gtctgtctac gaggaaaaac tgaaacagtt tgaagagcgc
     5161 ttagcagaag aaaggcatag tcgcctagaa gatcggaaaa ggcagcggaa agaagaacgc
     5221 aaaataactt attacagaga aaaggaagaa gaagagcaga ggagggcaga ggagcagatg
     5281 ctcaaagagc gagaagaaag agaacgtgct gagagagcaa aacgggagga agaacttaga
     5341 gagtatcagg agcgagtcaa gaaactagaa gaagtagaaa ggaaaaagcg gcaaagagag
     5401 ctggaaattg aagaaaggga aaggcgcaga gaggaagaaa ggagacttgg tgatgatcca
     5461 ctttctagga aggactctcg gtggggagat agagattcag aaggcacctg gaggaaagga
     5521 ccagaagctg actctgagtg gagaagaggc ccaccagaaa aggagtggag acgagaaact
     5581 cgggatgatg agaggcctca caggagagat gaggatcggc tgaggcgctt ggggggtgac
     5641 gatgaagaga gagagtcttc tcttagaccg gatgatgatc gtatccccag gcgtggcctg
     5701 gatgatgaca gagggcctag acgtggtccc gatgaagata gattttctcg tagaggaaca
     5761 gatgatgacc gtccttcctg gcgtaatgca gatgatgata ggcctcccag gcgaattggt
     5821 gatgatgaca ggggaagctg gcgtcacaca gatgacgaca gaccacccag acggggactg
     5881 gatgatgaga gaggaagctg gcgtacagca gatgaggaca gaggacctag acgagggatg
     5941 gatgatgacc gggggccaag acgaggaggt gctgatgatg aacggtcatc ctggcggaat
     6001 gctgatgatg ataggggtcc caggagaggc atggatgatg ataggggtcc caggcgaggg
     6061 ctggatgatg accgaggacc ttggaggaat gctgctgaag ataggatttc caggagaggt
     6121 gcagatgatg accgagggcc ttggagaaat atggatgatg atagggttcc tagaaggggt
     6181 gatgatgcaa gacctggtcc ttggagacca tttgtcaagc caggtggatg gagagagaaa
     6241 gaaaaggcta gagaagagag ttggggtcca cctcgagaat caagaccatc agaagaacgt
     6301 gaatgggata gagacaaaga gaaggacaga gataatcaag atcgagagga gaatgacaaa
     6361 gaccttgaac gagataggga cagagagaga gatggggacc gggaggatcg attcagaaga
     6421 cccagggatg aaggtggctg gagaagagga ccagcagaag aatcttcaag ctggagagat
     6481 tcaagtcgcc gtgatgatag ggacagggaa gaccgtcgac gggatagaga tgatcgtcgt
     6541 gatctgagag acctgagaga cagaagggat ttaagagatg atagagatcg gagaggacct
     6601 cccctcagat cagagcgaga agaagcaagc tcttggagac gcactgatga caggaaagat
     6661 gaccggactg aagagaggga tccacctcgt cgtgttcctc ccccagctct ttcaagagat
     6721 cgagaaagag agcgagaacg agaaggtgag aaagagaaag catcctggag agctgagaaa
     6781 gatagggagt cccttcgtcg tactaagaat gaaactgatg aagatggatg gaccacagta
     6841 cgacgtctcg aggactacaa agaccatgac ggtgattata aagatcatga catcgactac
     6901 aaggatgacg atgacaagta ggatcccgcc cctctccctc ccccccccct aacgttactg
     6961 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     7021 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     7081 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     7141 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     7201 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     7261 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     7321 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     7381 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     7441 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     7501 aagcttgcca caacccacaa ggagacgacc ttccatgacc gagtacaagc ccacggtgcg
     7561 cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga
     7621 ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct
     7681 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     7741 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     7801 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     7861 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     7921 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     7981 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     8041 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     8101 cacctggtgc atgacccgca agcccggtgc ctagacgcgt ctggaacaat caacctctgg
     8161 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     8221 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     8281 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     8341 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     8401 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     8461 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     8521 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     8581 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     8641 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     8701 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga
     8761 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     8821 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     8881 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg
     8941 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     9001 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     9061 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     9121 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     9181 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     9241 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     9301 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     9361 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     9421 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9481 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9541 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     9601 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct
     9661 agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     9721 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9781 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9841 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9901 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9961 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    10021 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    10081 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    10141 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    10201 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10261 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10321 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10381 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10441 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10501 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    10561 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10621 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10681 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10741 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10801 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10861 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10921 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10981 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    11041 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    11101 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    11161 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    11221 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    11281 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    11341 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    11401 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    11461 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    11521 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11581 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11641 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11701 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11761 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11821 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac
    11881 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    11941 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    12001 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    12061 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    12121 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
    12181 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
