LOCUS       .                       9851 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2842..3558
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     CDS             3652..4608
                     /label="TurboID"
                     /note="mutant of the E. coli biotin ligase BioID engineered
                     for improved catalytic efficiency (Branon et al., 2018)"
     promoter        4639..5138
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5159..5755
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    5772..6360
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6567..7200
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7329..7345)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7353..7369)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7377..7407)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7422..7443)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7731..8319)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8493..9350)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(9351..9455)
                     /label="AmpR promoter"
     polyA_signal    9503..9637
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 gtagatctag cggccgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2881 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2941 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3001 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     3061 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     3121 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     3181 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     3241 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     3301 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     3361 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     3421 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     3481 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3541 atggacgagc tgtacaaggg cggtggaggc agtggtggag gcggtagcga attcgccacc
     3601 atgggcaagc ccatccccaa ccccctgctg ggcctggaca gcaccgctag caaagacaat
     3661 actgtgcctc tgaagctgat cgctctcctg gctaatggcg agttccatag tggcgaacag
     3721 ctgggagaaa ccctgggcat gtccagggcc gctatcaaca agcacattca gactctgcgc
     3781 gactggggcg tggacgtgtt caccgtgccc ggaaagggct actctctgcc cgagcctatc
     3841 ccgctgctga acgctaaaca gattctggga cagctggacg gcgggagcgt ggcagtcctg
     3901 cctgtggtcg actccaccaa tcagtacctg ctggatcgaa tcggcgagct gaagagtggg
     3961 gatgcttgca ttgcagaata tcagcaggca gggagaggaa gcagagggag gaaatggttc
     4021 tctccttttg gagctaacct gtacctgagt atgttttggc gcctgaagcg gggaccagca
     4081 gcaatcggcc tgggcccggt catcggaatt gtcatggcag aagcgctgcg aaagctggga
     4141 gcagacaagg tgcgagtcaa atggcccaat gacctgtatc tgcaggatag aaagctggca
     4201 ggcatcctgg tggagctggc cggaataaca ggcgatgctg cacagatcgt cattggcgcc
     4261 gggattaacg tggctatgag gcgcgtggag gaaagcgtgg tcaatcaggg ctggatcaca
     4321 ctgcaggaag cagggattaa cctggacagg aatactctgg ccgctacgct gatccgagag
     4381 ctgcgggcag ccctggaact gttcgagcag gaaggcctgg ctccatatct gccacggtgg
     4441 gagaagctgg ataacttcat caatagaccc gtgaagctga tcattgggga caaagagatt
     4501 ttcgggatta gccgggggat tgataaacag ggagccctgc tgctggaaca ggacggagtt
     4561 atcaaaccct ggatgggcgg agaaatcagt ctgcggtctg ccgaaaagta ggatcccgcg
     4621 actctagata attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     4681 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     4741 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     4801 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     4861 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     4921 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     4981 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     5041 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     5101 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     5161 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     5221 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     5281 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     5341 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     5401 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     5461 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     5521 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     5581 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     5641 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     5701 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc
     5761 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5821 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5881 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5941 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6001 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6061 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6121 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     6181 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6241 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6301 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6361 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     6421 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     6481 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6541 ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga
     6601 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     6661 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     6721 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     6781 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     6841 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     6901 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     6961 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     7021 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7081 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7141 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7201 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7261 agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg
     7321 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7381 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7441 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7501 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7561 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7621 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7681 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7741 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7801 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7861 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7921 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7981 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8041 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8101 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8161 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8221 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8281 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8341 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8401 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8461 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8521 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8581 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8641 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8701 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8761 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8821 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8881 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8941 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9001 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9061 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9121 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9181 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9241 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9301 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9361 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9421 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9481 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
     9541 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9601 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
     9661 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
     9721 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
     9781 aatatgtact acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca
     9841 aacttagtag t
//
