LOCUS       V014380                 9431 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014380
VERSION     V014380
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2833..2898
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     promoter        2909..3438
                     /label="mCMV promoter"
                     /note="mouse cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             3454..4146
                     /label="ZsGreen1"
                     /note="Zoanthus green fluorescent protein"
     misc_feature    4160..4734
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4740..5336
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    5353..5941
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6149..6782
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6911..6927)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6935..6951)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6959..6989)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7004..7025)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7313..7898)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8072..8929)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8930..9034)
                     /label="AmpR promoter"
     polyA_signal    9082..9216
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt
     2821 atttgcggcc gcgactacaa agaccatgac ggtgattata aagatcatga catcgattac
     2881 aaggatgacg atgacaagtg aggatccgat atactgagtc attagggact ttccaatggg
     2941 ttttgcccag tacataaggt caataggggt gaatcaacag gaaagtccca ttggagccaa
     3001 gtacactgag tcaataggga ctttccattg ggttttgccc agtacaaaag gtcaataggg
     3061 ggtgagtcaa tgggtttttc ccattattgg cacgtacata aggtcaatag gggtgagtca
     3121 ttgggttttt ccagccaatt taattaaaac gccatgtact ttcccaccat tgacgtcaat
     3181 gggctattga aactaatgca acgtgacctt taaacggtac tttcccatag ctgattaatg
     3241 ggaaagtacc gttctcgagc caatacacgt caatgggaag tgaaagggca gccaaaacgt
     3301 aacaccgccc cggttttccc ctggaaattc catattggca cgcattctat tggctgagct
     3361 gcgttctacg tgggtataag aggcgcgacc agcgtcggta ccgtcgcagt cttcggtctg
     3421 accaccgtag aacgcagatc accggtcgcc accatggccc agtccaagca cggcctgacc
     3481 aaggagatga ccatgaagta ccgcatggag ggctgcgtgg acggccacaa gttcgtgatc
     3541 accggcgagg gcatcggcta ccccttcaag ggcaagcagg ccatcaacct gtgcgtggtg
     3601 gagggcggcc ccttgccctt cgccgaggac atcttgtccg ccgccttcat gtacggcaac
     3661 cgcgtgttca ccgagtaccc ccaggacatc gtcgactact tcaagaactc ctgccccgcc
     3721 ggctacacct gggaccgctc cttcctgttc gaggacggcg ccgtgtgcat ctgcaacgcc
     3781 gacatcaccg tgagcgtgga ggagaactgc atgtaccacg agtccaagtt ctacggcgtg
     3841 aacttccccg ccgacggccc cgtgatgaag aagatgaccg acaactggga gccctcctgc
     3901 gagaagatca tccccgtgcc caagcagggc atcttgaagg gcgacgtgag catgtacctg
     3961 ctgctgaagg acggtggccg cttgcgctgc cagttcgaca ccgtgtacaa ggccaagtcc
     4021 gtgccccgca agatgcccga ctggcacttc atccagcaca agctgacccg cgaggaccgc
     4081 agcgacgcca agaaccagaa gtggcacctg accgagcacg ccatcgcctc cggctccgcc
     4141 ttgccctaag gcgcgcccgc ccctctccct cccccccccc taacgttact ggccgaagcc
     4201 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     4261 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     4321 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     4381 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     4441 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     4501 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctca
     4561 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     4621 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct
     4681 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aataccggta
     4741 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     4801 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     4861 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     4921 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     4981 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     5041 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     5101 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     5161 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     5221 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     5281 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa
     5341 cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     5401 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     5461 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     5521 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     5581 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     5641 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     5701 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     5761 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     5821 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     5881 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     5941 cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata
     6001 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     6061 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6121 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa
     6181 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     6241 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     6301 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     6361 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     6421 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     6481 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     6541 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     6601 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6661 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6721 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6781 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     6841 agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc tagagcttgg
     6901 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6961 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7021 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7081 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7141 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7201 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7261 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7321 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7381 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7441 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7501 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7561 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7621 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7681 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7741 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7801 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt
     7861 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7921 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7981 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8041 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8101 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8161 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8221 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8281 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8341 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8401 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8461 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8521 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8581 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8641 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8701 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8761 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8821 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8881 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8941 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9001 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9061 acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc
     9121 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9181 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     9241 aatcatccca aacttcccac cccataccct attaccactg ccaattacct agtggtttca
     9301 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
     9361 aatatgtact acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca
     9421 aacttagtag t
//
