LOCUS       V024377                 7808 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024377
VERSION     V024377
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     promoter        2206..2520
                     /note="Tet-responsive promoter&nbsp;PTight, consisting of
                     seven tet operator&nbsp;sequences followed by the minimal
                     CMV promoter"
                     /label="tight TRE promoter"
     CDS             2546..3712
                     /label="KLF17(NM_173484)"
                     /note="KLF17(NM_173484)"
                     /gene="KLF17"
     CDS             complement(4196..4207)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /transl_table=1
                     /translation="IEGR"
                     /label="Factor Xa site"
     LTR             4526..5159
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(5288..5304)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5312..5328
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5336..5366)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5381..5402
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5690..6275)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6446..7306)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7307..7411)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    7459..7593
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
     2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2521 tggagaagga tccgcggccg ccaccatgta cggccgaccg caggctgaga tggaacagga
     2581 ggctggggag ctgagccggt ggcaggcggc gcaccaggct gcccaggata acgagaactc
     2641 agcgcccatc ttgaacatgt cttcatcttc tggaagctct ggagtgcaca cctcttggaa
     2701 ccaaggccta ccaagcattc agcactttcc tcacagcgca gagatgctgg ggtccccttt
     2761 ggtgtctgtt gaggcgccgg ggcagaatgt gaatgaaggg gggccacagt tcagtatgcc
     2821 actgcctgag cgtggtatga gctactgccc ccaagcgact ctcactcctt cccggatgat
     2881 ttactgtcag agaatgtctc cccctcagca agagatgacg attttcagtg ggccccaact
     2941 aatgcccgta ggagagccca atattccaag ggtagccagg cccttcggtg ggaatctaag
     3001 gatgcccccc aatgggctgc cagtctcggc ttccactgga atcccaataa tgtcccacac
     3061 tgggaaccct ccagtgcctt accctggcct ctcgacagta ccttctgacg aaacattgtt
     3121 gggcccgact gtgccttcca ctgaggccca ggcagtgctc ccctccatgg ctcagatgtt
     3181 gcccccgcaa gatgcccatg accttgggat gcccccagct gagtcccagt cattgctggt
     3241 tttaggatct caggactctc ttgtcagtca gccagactct caagaaggcc catttctacc
     3301 agagcagccc ggacctgctc cacagacagt agagaagaac tccaggcctc aggaagggac
     3361 tggtagaagg ggctcctcag aggcaaggcc ttactgctgc aactacgaga actgcggaaa
     3421 agcttatacc aaacgctccc acctcgtgag ccaccagcgc aagcacacag gtgagaggcc
     3481 atattcttgc aactgggaaa gttgttcatg gtctttcttc cgttctgatg agcttagacg
     3541 acatatgcgg gtacacacca gatatcgacc atataaatgt gatcagtgca gccgggagtt
     3601 catgaggtct gaccatctca agcaacacca gaagactcat cggccgggac cctcagaccc
     3661 acaggccaac aacaacaatg gagagcagga cagtcctcct gctgctggtc cttagacgcg
     3721 tgaattccaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3781 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3841 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3901 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3961 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4021 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4081 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     4141 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4201 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4261 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaag
     4321 tttaaacctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     4381 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggtttttcca
     4441 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     4501 tttttaaaag aaaagagggg actgctggaa gggctaattc actcccaacg aagacaagat
     4561 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     4621 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     4681 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     4741 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     4801 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     4861 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     4921 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     4981 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5041 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5101 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5161 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     5221 gagtgagagg ccttgacatt gctagcgttt taccgtcgac ctctagctag agcttggcgt
     5281 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5341 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5401 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5461 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5521 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5581 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5641 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5701 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5761 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5821 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5881 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5941 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6001 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6061 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6121 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6181 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca
     6241 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6301 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6361 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6421 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6481 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6541 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6601 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6661 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6721 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6781 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6841 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6901 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6961 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7021 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7081 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7141 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7201 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7261 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7321 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7381 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     7441 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7501 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7561 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     7621 catcccaaac ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt
     7681 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     7741 atgtactaca aacttagtag tttttaaaga aattgtattt gttaaatatg tactacaaac
     7801 ttagtagt
//
