LOCUS       V025320                 8108 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025320
VERSION     V025320
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     promoter        2206..2520
                     /note="Tet-responsive promoter&nbsp;PTight, consisting of
                     seven tet operator&nbsp;sequences followed by the minimal
                     CMV promoter"
                     /label="tight TRE promoter"
     CDS             2546..4012
                     /label="FOXG1(NM_005249)"
                     /note="FOXG1(NM_005249)"
                     /gene="FOXG1"
     CDS             complement(4496..4507)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /transl_table=1
                     /translation="IEGR"
                     /label="Factor Xa site"
     LTR             4826..5459
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(5588..5604)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5612..5628
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5636..5666)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5681..5702
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5990..6575)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6746..7606)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7607..7711)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    7759..7893
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
     2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2521 tggagaagga tccgcggccg ccaccatgct ggacatggga gataggaaag aggtgaaaat
     2581 gatccccaag tcctcgttca gcatcaacag cctggtgccc gaggcggtcc agaacgacaa
     2641 ccaccacgcg agccacggcc accacaacag ccaccacccc cagcaccacc accaccacca
     2701 ccaccatcac caccacccgc cgccgcccgc cccgcaaccg ccgccgccgc cgcagcagca
     2761 gcagccgccg ccgccgccgc ccccggcacc gcagcccccc cagacgcggg gcgccccggc
     2821 cgccgacgac gacaagggcc cccagcagct gctgctcccg ccgccgccac cgccaccacc
     2881 ggccgccgcc ctggacgggg ctaaagcgga cgggctgggc ggcaagggcg agccgggcgg
     2941 cgggccgggg gagctggcgc ccgtcgggcc ggacgagaag gagaagggcg ccggcgccgg
     3001 gggggaggag aagaaggggg cgggcgaggg cggcaaggac ggggaggggg gcaaggaggg
     3061 cgagaagaag aacggcaagt acgagaagcc gccgttcagc tacaacgcgc tcatcatgat
     3121 ggccatccgg cagagccccg agaagcggct cacgctcaac ggcatctacg agttcatcat
     3181 gaagaacttc ccttactacc gcgagaacaa gcagggctgg cagaactcca tccgccacaa
     3241 tctgtccctc aacaagtgct tcgtgaaggt gccgcgccac tacgacgacc cgggcaaggg
     3301 caactactgg atgctggacc cgtcgagcga cgacgtgttc atcggcggca ccacgggcaa
     3361 gctgcggcgc cgctccacca cctcgcgggc caagctggcc ttcaagcgcg gtgcgcgcct
     3421 cacctccacc ggcctcacct tcatggaccg cgccggctcc ctctactggc ccatgtcgcc
     3481 cttcctgtcc ctgcaccacc cccgcgccag cagcactttg agttacaacg gcaccacgtc
     3541 ggcctacccc agccacccca tgccctacag ctccgtgttg actcagaact cgctgggcaa
     3601 caaccactcc ttctccaccg ccaacggcct gagcgtggac cggctggtca acggggagat
     3661 cccgtacgcc acgcaccacc tcacggccgc cgcgctagcc gcctcggtgc cctgcggcct
     3721 gtcggtgccc tgctctggga cctactccct caacccctgc tccgtcaacc tgctcgcggg
     3781 ccagaccagt tactttttcc cccacgtccc gcacccgtca atgacttcgc agagcagcac
     3841 gtccatgagc gccagggccg cgtcctcctc cacgtcgccg caggccccct cgaccctgcc
     3901 ctgtgagtct ttaagaccct ctttgccaag ttttacgacg ggactgtctg ggggactgtc
     3961 tgattatttc acacatcaaa atcaggggtc ttcttccaac cctttaatac attaaacgcg
     4021 tgaattccaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4081 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4141 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4201 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4261 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4321 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4381 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     4441 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4501 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4561 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaag
     4621 tttaaacctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     4681 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggtttttcca
     4741 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     4801 tttttaaaag aaaagagggg actgctggaa gggctaattc actcccaacg aagacaagat
     4861 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     4921 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     4981 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     5041 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     5101 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     5161 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     5221 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     5281 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5341 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5401 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5461 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     5521 gagtgagagg ccttgacatt gctagcgttt taccgtcgac ctctagctag agcttggcgt
     5581 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5641 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5701 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5761 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5821 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5881 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5941 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6001 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6061 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6121 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6181 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6241 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6301 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6361 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6421 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6481 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca
     6541 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6601 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6661 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6721 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6781 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6841 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6901 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6961 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7021 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7081 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7141 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7201 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7261 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7321 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7381 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7441 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7501 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7561 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7621 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7681 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     7741 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7801 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7861 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     7921 catcccaaac ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt
     7981 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     8041 atgtactaca aacttagtag tttttaaaga aattgtattt gttaaatatg tactacaaac
     8101 ttagtagt
//
