LOCUS       V014482                11696 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014482
VERSION     V014482
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             5661..5684
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             5745..6461
                     /label="EGFP"
                     /note="enhanced GFP"
     promoter        6484..6983
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             7004..7600
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    7617..8205
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             8412..9045
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(9222..9252)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9267..9288)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9576..10164)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10338..11195)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11196..11300)
                     /label="AmpR promoter"
     polyA_signal    11348..11482
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgcg accctccggg acggccgggg cagcgctcct ggcgctgctg gctgcgctct
     2881 gcccggcgag tcgggctctg gaggaaaaga aaggtaatta tgtggtgaca gatcacggct
     2941 cgtgcgtccg agcctgtggg gccgacagct atgagatgga ggaagacggc gtccgcaagt
     3001 gtaagaagtg cgaagggcct tgccgcaaag tgtgtaacgg aataggtatt ggtgaattta
     3061 aagactcact ctccataaat gctacgaata ttaaacactt caaaaactgc acctccatca
     3121 gtggcgatct ccacatcctg ccggtggcat ttaggggtga ctccttcaca catactcctc
     3181 ctctggatcc acaggaactg gatattctga aaaccgtaaa ggaaatcaca gggtttttgc
     3241 tgattcaggc ttggcctgaa aacaggacgg acctccatgc ctttgagaac ctagaaatca
     3301 tacgcggcag gaccaagcaa catggtcagt tttctcttgc agtcgtcagc ctgaacataa
     3361 catccttggg attacgctcc ctcaaggaga taagtgatgg agatgtgata atttcaggaa
     3421 acaaaaattt gtgctatgca aatacaataa actggaaaaa actgtttggg acctccggtc
     3481 agaaaaccaa aattataagc aacagaggtg aaaacagctg caaggccaca ggccaggtct
     3541 gccatgcctt gtgctccccc gagggctgct ggggcccgga gcccagggac tgcgtctctt
     3601 gccggaatgt cagccgaggc agggaatgcg tggacaagtg caaccttctg gagggtgagc
     3661 caagggagtt tgtggagaac tctgagtgca tacagtgcca cccagagtgc ctgcctcagg
     3721 ccatgaacat cacctgcaca ggacggggac cagacaactg tatccagtgt gcccactaca
     3781 ttgacggccc ccactgcgtc aagacctgcc cggcaggagt catgggagaa aacaacaccc
     3841 tggtctggaa gtacgcagac gccggccatg tgtgccacct gtgccatcca aactgcacct
     3901 acggatgcac tgggccaggt cttgaaggct gtccaacgaa tgggcctaag atcccgtcca
     3961 tcgccactgg gatggtgggg gccctcctct tgctgctggt ggtggccctg gggatcggcc
     4021 tcttcatgcg aaggcgccac atcgttcgga agcgcacgct gcggaggctg ctgcaggaga
     4081 gggagcttgt ggagcctctt acacccagtg gagaagctcc caaccaagct ctcttgagga
     4141 tcttgaagga aactgaattc aaaaagatca aagtgctggg ctccggtgcg ttcggcacgg
     4201 tgtataaggg actctggatc ccagaaggtg agaaagttaa aattcccgtc gctatcaagg
     4261 aattaagaga agcaacatct ccgaaagcca acaaggaaat cctcgatgaa gcctacgtga
     4321 tggccagcgt ggacaacccc cacgtgtgcc gcctgctggg catctgcctc acctccaccg
     4381 tgcagctcat cacgcagctc atgcccttcg gctgcctcct ggactatgtc cgggaacaca
     4441 aagacaatat tggctcccag tacctgctca actggtgtgt gcagatcgca aagggcatga
     4501 actacttgga ggaccgtcgc ttggtgcacc gcgacctggc agccaggaac gtactggtga
     4561 aaacaccgca gcatgtcaag atcacagatt ttgggctggc caaactgctg ggtgcggaag
     4621 agaaagaata ccatgcagaa ggaggcaaag tgcctatcaa gtggatggca ttggaatcaa
     4681 ttttacacag aatctatacc caccagagtg atgtctggag ctacggggtg actgtttggg
     4741 agttgatgac ctttggatcc aagccatatg acggaatccc tgccagcgag atctcctcca
     4801 tcctggagaa aggagaacgc ctccctcagc cacccatatg taccatcgat gtctacatga
     4861 tcatggtcaa gtgctggatg atagacgcag atagtcgccc aaagttccgt gagttgatca
     4921 tcgaattctc caaaatggcc cgagaccccc agcgctacct tgtcattcag ggggatgaaa
     4981 gaatgcattt gccaagtcct acagactcca acttctaccg tgccctgatg gatgaagaag
     5041 acatggacga cgtggtggat gccgacgagt acctcatccc acagcagggc ttcttcagca
     5101 gcccctccac gtcacggact cccctcctga gctctctgag tgcaaccagc aacaattcca
     5161 ccgtggcttg cattgataga aatgggctgc aaagctgtcc catcaaggaa gacagcttct
     5221 tgcagcgata cagctcagac cccacaggcg ccttgactga ggacagcata gacgacacct
     5281 tcctcccagt gcctgaatac ataaaccagt ccgttcccaa aaggcccgct ggctctgtgc
     5341 agaatcctgt ctatcacaat cagcctctga accccgcgcc cagcagagac ccacactacc
     5401 aggaccccca cagcactgca gtgggcaacc ccgagtatct caacactgtc cagcccacct
     5461 gtgtcaacag cacattcgac agccctgccc actgggccca gaaaggcagc caccaaatta
     5521 gcctggacaa ccctgactac cagcaggact tctttcccaa ggaagccaag ccaaatggca
     5581 tctttaaggg ctccacagct gaaaatgcag aatacctaag ggtcgcgcca caaagcagtg
     5641 aatttattgg agcagaattc gattacaagg atgacgacga taaggagggc agaggaagtc
     5701 ttctaacatg cggtgacgtg gaggagaatc ccggccctgt taacatggtg agcaagggcg
     5761 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     5821 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     5881 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     5941 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     6001 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     6061 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     6121 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     6181 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     6241 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     6301 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     6361 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     6421 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtagtaatct agataattct
     6481 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     6541 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     6601 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     6661 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     6721 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     6781 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     6841 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     6901 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     6961 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
     7021 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     7081 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     7141 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     7201 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     7261 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     7321 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     7381 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     7441 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     7501 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     7561 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc
     7621 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     7681 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     7741 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     7801 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     7861 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     7921 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     7981 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     8041 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     8101 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     8161 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg
     8221 cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     8281 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     8341 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     8401 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     8461 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     8521 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     8581 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     8641 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     8701 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     8761 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     8821 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     8881 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8941 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9001 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     9061 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     9121 gacattgcta gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     9181 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     9241 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     9301 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9361 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9421 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9481 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9541 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9601 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9661 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9721 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9781 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9841 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9901 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9961 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    10021 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    10081 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    10141 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    10201 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    10261 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    10321 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    10381 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    10441 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    10501 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    10561 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10621 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10681 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10741 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10801 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10861 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10921 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10981 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    11041 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    11101 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    11161 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    11221 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    11281 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
    11341 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    11401 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    11461 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    11521 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
    11581 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    11641 cttagtagtt tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
