LOCUS       V014345                10286 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014345
VERSION     V014345
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10286)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             4332..5039
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     promoter        5074..5573
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5594..6190
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6207..6795
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7002..7635
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7812..7842)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7857..7878)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8166..8754)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8928..9785)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9786..9890)
                     /label="AmpR promoter"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
     2821 ccaccatgtg gctgcgcggc ctgttcggcg gcctgttctt cctgttcctg ctgtgcagct
     2881 gcgtggccct gttcgtgctg cacagcctgt acttcatctt ccgcaccgcc agccagaccg
     2941 agaagaccga gagcaagacc gtgcacgacg accagctgtt catccccgcc ctggtgatct
     3001 gcaaccgcat gcccttcagc caggacggcc tgaacaacgt gaacgtgaac ctgcgccagg
     3061 acagcgccct gcgctacctg ctggagtgga ccaaccccag cctgcgcgag gccgccgact
     3121 acgtggcccc cagcgccgac ctgatgaacc agggccagaa caccgtgctg cagtacatca
     3181 cccagagcac ccgcaaccag accatccaga acatgcagta ccagtgccag agcgtgatca
     3241 acagctgcac ctaccagggc atccagctga gcagcttcga ctgctgccgc aacctgctga
     3301 gcatgatccc cagcaccaac ggcctgtgct gggtgtggaa ggacagcacc atgtggcaga
     3361 acagcaccgg catcaaccgc cagttcagca tcaccttcca gatgacccgc aacagctggt
     3421 tcagcaccta cgtgcccacc caccccggcg tggacatcta cctgcgcgag gacggcaacg
     3481 acgtgatgcg catggccacc gagctggaga accccatccg cctgctggac aagcgcggcg
     3541 tgcgcctgca gatgcgcaag accaagaagg ccgacatccg ccgcaccagc tgcggctacg
     3601 ccctgggcga cgcccgccgc agcgacgagc acgccttcaa gaacaaccac accaactacc
     3661 tgatgtgcaa catgctggtg gccatgcgct actgcagctg ccaccccctg atggccgagc
     3721 tgatccacta cgaccccagc agctaccgcg acttcctgct gcgcgtgacc cagaccagcg
     3781 tgtgcagcgt ggacgcctac gacaactgcg cccgccgcta catcgacctg acccgcatcg
     3841 agaactggga ggaggacatc cccaaggacc tgcccggcta cgaggacgcc aagaagtgcc
     3901 gcaacgacaa ccagcgcagc tgcctgatca ccagctaccc cggcaccatc gagggctacg
     3961 acctgcccga ggagtaccgc accacccagg actacgtgag ccgcctggtg ctggagtaca
     4021 gcaccatgcg caccaccgag atcctggtga gcaaggaccc caacatctac gagctgctga
     4081 gcttcatcgg ctacaacatg gccctgtggt tcaccgtggg ccacatcctg tggagcatgt
     4141 tctggtacgc caccggcctg tgctgcccca agcgccagag cagcaaccgc atcagccccg
     4201 agatccgccg caagcgcagc gtgagcagcc ccgagcccat cgtggtggag caccgcccca
     4261 gccaggacgt ggccagcggc gacaccggag gtggcggtag tggtggcgga ggtagcggcg
     4321 gtggagggtc catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     4381 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     4441 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     4501 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     4561 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     4621 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     4681 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     4741 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     4801 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     4861 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     4921 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     4981 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     5041 agtaaggatc ccgcgactct agataattct accgggtagg ggaggcgctt ttcccaaggc
     5101 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     5161 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     5221 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     5281 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     5341 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     5401 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     5461 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     5521 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     5581 gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     5641 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     5701 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     5761 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     5821 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     5881 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     5941 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     6001 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     6061 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     6121 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     6181 gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat
     6241 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6301 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6361 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6421 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6481 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6541 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6601 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6661 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6721 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6781 gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat
     6841 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     6901 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     6961 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc
     7021 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     7081 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     7141 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     7201 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     7261 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     7321 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     7381 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     7441 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7501 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7561 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7621 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7681 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct
     7741 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7801 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7861 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7921 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7981 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8041 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8101 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8161 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8221 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8281 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8341 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8401 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8461 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8521 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8581 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8641 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8701 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8761 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8821 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8881 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8941 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9001 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9061 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9121 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9181 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9241 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9301 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9361 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9421 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9481 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9541 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9601 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9661 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9721 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9781 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9841 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9901 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa
     9961 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10021 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    10081 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    10141 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
    10201 aaattgtatt tgttaaatat gtactacaaa cttagtagtt tttaaagaaa ttgtatttgt
    10261 taaatatgta ctacaaactt agtagt
//
