LOCUS V029846 7654 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029846 VERSION V029846 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 1..634 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 681..806 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1303..1536 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1721..1765 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 2028..2143 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" promoter 2206..2520 /note="Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter" /label="tight TRE promoter" CDS 2548..3558 /label="CSNK1A1(NM_001892)" /note="CSNK1A1(NM_001892)" /gene="CSNK1A1" CDS complement(4042..4053) /codon_start=1 /product="Factor Xa recognition and cleavage site" /transl_table=1 /translation="IEGR" /label="Factor Xa site" LTR 4372..5005 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" primer_bind complement(5134..5150) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5158..5174 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5182..5212) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5227..5248 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5536..6121) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6292..7152) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7153..7257) /gene="bla" /label="AmpR promoter" polyA_signal 7305..7439 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat 2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt 2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa 2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca 2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt 2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 2521 tggagaagga tccgcggccg cgccaccatg gcgagtagca gcggctccaa ggctgaattc 2581 attgtcggag ggaaatataa actggtacgg aagatcgggt ctggctcctt cggggacatc 2641 tatttggcga tcaacatcac caacggcgag gaagtggcag tgaagctaga atctcagaag 2701 gccaggcatc cccagttgct gtacgagagc aagctctata agattcttca aggtggggtt 2761 ggcatccccc acatacggtg gtatggtcag gaaaaagact acaatgtact agtcatggat 2821 cttctgggac ctagcctcga agacctcttc aatttctgtt caagaaggtt cacaatgaaa 2881 actgtactta tgttagctga ccagatgatc agtagaattg aatatgtgca tacaaagaat 2941 tttatacaca gagacattaa accagataac ttcctaatgg gtattgggcg tcactgtaat 3001 aagttattcc ttattgattt tggtttggcc aaaaagtaca gagacaacag gacaaggcaa 3061 cacataccat acagagaaga taaaaacctc actggcactg cccgatatgc tagcatcaat 3121 gcacatcttg gtattgagca gagtcgccga gatgacatgg aatcattagg atatgttttg 3181 atgtatttta atagaaccag cctgccatgg caagggctaa aggctgcaac aaagaaacaa 3241 aaatatgaaa agattagtga aaagaagatg tccacgcctg ttgaagtttt atgtaagggg 3301 tttcctgcag aatttgcgat gtacttaaac tattgtcgtg ggctacgctt tgaggaagcc 3361 ccagattaca tgtatctgag gcagctattc cgcattcttt tcaggaccct gaaccatcaa 3421 tatgactaca catttgattg gacaatgtta aagcagaaag cagcacagca ggcagcctct 3481 tccagtgggc agggtcagca ggcccaaacc cccacaggca agcaaactga caaaaccaag 3541 agtaacatga aaggtttcta aacgcgtgaa ttccaacctc tggattacaa aatttgtgaa 3601 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 3661 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 3721 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 3781 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 3841 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 3901 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 3961 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg 4021 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 4081 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 4141 ctttgggccg cctccccgcc tggaagttta aacctgcagt cgagacctag aaaaacatgg 4201 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca 4261 agaggaggag gaggtgggtt tttccagtca cacctcaggt acctttaaga ccaatgactt 4321 acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg ctggaagggc 4381 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct 4441 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg 4501 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag 4561 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag 4621 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc 4681 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact 4741 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat 4801 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag 4861 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 4921 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 4981 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta 5041 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta gcgttttacc 5101 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 5161 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 5221 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 5281 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 5341 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 5401 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 5461 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 5521 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 5581 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 5641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 5701 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 5761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 5821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 5881 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 5941 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 6001 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 6061 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 6121 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 6181 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 6241 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 6301 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 6361 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 6421 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 6481 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 6541 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 6601 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 6661 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 6721 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 6781 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 6841 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 6901 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 6961 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 7021 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 7081 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 7141 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 7201 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 7261 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag 7321 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 7381 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca 7441 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca 7501 ctgccaatta cctagtggtt tcatttactc taaacctgtg attcctctga attattttca 7561 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagtttt taaagaaatt 7621 gtatttgtta aatatgtact acaaacttag tagt //