LOCUS       V004907                 9698 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004907
VERSION     V004907
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Goncalves MA, Janssen JM, Holkers M, de Vries AA.
  TITLE     Rapid and sensitive lentivirus vector-based conditional gene
            expression assay to monitor and quantify cell fusion activity
  JOURNAL   PLoS ONE 5 (6), E10954 (2010)
   PUBMED   20532169
REFERENCE   2  (bases 1 to 9698)
  AUTHORS   Goncalves MAF.V., Janssen JM, Holkers M, de Vries AAF.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2009) Molecular Cell Biology, Leiden University
            Medical Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
            Netherlands
REFERENCE   3  (bases 1 to 9698)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9698)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2010"; volume: "5"; issue: "6"; pages: "E10954"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-DEC-2009) Molecular Cell Biology, Leiden University Medical
            Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1799..1916
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    complement(1943..1990)
                     /label="FRT"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    complement(1943..1976)
                     /note="FRT site; Saccharomyces cerevisiae site-specific
                     recombinase FLP recognition target site"
     regulatory      complement(2003..2506)
                     /label="Homo sapiens GAPDH promoter"
                     /note="Homo sapiens GAPDH promoter"
                     /regulatory_class="promoter"
     polyA_signal    complement(2907..2962)
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     CDS             complement(3129..4778)
                     /label="luciferase"
                     /note="firefly luciferase"
     protein_bind    complement(4837..4884)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      complement(4936..5321)
                     /label="murine metallothionein polyadenylation signal"
                     /note="murine metallothionein polyadenylation signal"
                     /regulatory_class="polyA_signal_sequence"
     CDS             5682..6122
                     /gene="X"
                     /label="Protein X"
                     /note="Protein X from Hepatitis B virus genotype D subtype
                     ayw (isolate France/Tiollais/1979). Accession#: P03165"
     LTR             6214..6447
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6519..6653
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6680..6815
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6836..6854)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6864..6880)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      7025..7480
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7509..7613
                     /label="AmpR promoter"
     misc_feature    7798..8175
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"
     rep_origin      8644..9232
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9520..9541
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9556..9586
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9594..9610
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9618..9634
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9655..9673
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1861 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     1921 ggggatcctc tagagtcagc ttgaagttcc tatactttct agagaatagg aacttcggaa
     1981 tagtaacttc aatgatctcg agcaggagga gcagagagcg aagcgggagg ctgcgggctc
     2041 aatttataga aaccgggggc gcggcggcag gcggtgactc ggaccccgcc tcccgccagg
     2101 ctcagccagt cccagcccaa ggtcttgagg cctgagctac gtgcgcccgt aaaaccgcta
     2161 gtagccgggc cctactttct ccccgctttt ttttttccgc agccgcctgg ttcaactggg
     2221 cacgcaccga gcgtgtgggc cccgacgtag ggggaaggga ctgagattgg cccgatggga
     2281 ggtgatcggt gctggttccc aggactggac tgtgggcagc aggacactag ggagtcaagg
     2341 acggggaccc ttacacgctt ggatgaaaca ggaggacttt gggaacgact gagatgggga
     2401 attggagccg gagcccagca ccgctcgcgg ctgcacgcgg cctgccccct cccctttctt
     2461 tctttcaaag gctgagaggc gggaaagttg gggaacttct cctcgaagct gatccaattg
     2521 caatgatcat catgacagat ctgcgcgcga tcgatgggat cagcttgggc tgcaggtcga
     2581 gggatcttca taagagaaga gggacagcta tgactgggag tagtcaggag aggaggaaaa
     2641 atctggctag taaaacatgt aaggaaaatt ttagggatgt taaagaaaaa aataacacaa
     2701 aacaaaatat aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga
     2761 cagcaggggg ctgtttcata tactgatgac ctctttatag ccacctttgt tcatggcagc
     2821 cagcatatgg catatgttgc caaactctaa accaaatact cattctgatg ttttaaatga
     2881 tttgccctcc catatgtcct tccgagtgag agacacaaaa aattccaaca cactattgca
     2941 atgaaaataa atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc
     3001 ccataatttt tggcagaggg aaaaagatct cagtggtatt tgtgagccag ggcattggcc
     3061 acaccagcca ccaccttttg ataggcagcc tgcacctgag gagtgaattg atccacgcgc
     3121 tagaattaca cggcgatctt tccgcccttc ttggccttta tgaggatctc tctgattttt
     3181 cttgcgtcga gttttccggt aagacctttc ggtacttcgt ccacaaacac aactcctccg
     3241 cgcaactttt tcgcggttgt tacttgactg gcgacgtaat ccacgatctc tttttccgtc
     3301 atcgtctttc cgtgctccaa aacaacaacg gcggcgggaa gttcaccggc gtcatcgtcg
     3361 ggaagacctg cgacacctgc gtcgaagatg ttggggtgtt ggagcaagat ggattccaat
     3421 tcagcgggag ccacctgata gcctttgtac ttaatcagag acttcaggcg gtcaacgatg
     3481 aagaagtgtt cgtcttcgtc ccagtaagct atgtctccag aatgtagcca tccatccttg
     3541 tcaatcaagg cgttggtcgc ttccggattg tttacataac cggacataat cataggacct
     3601 ctcacacaca gttcgcctct ttgattaacg cccagcgttt tcccggtatc cagatccaca
     3661 accttcgctt caaaaaatgg aacaacttta ccgaccgcgc ccggtttatc atccccctcg
     3721 ggtgtaatca gaatagctga tgtagtctca gtgagcccat atccttgcct gatacctggc
     3781 agatggaacc tcttggcaac cgcttccccg acttccttag agaggggagc gccaccagaa
     3841 gcaatttcgt gtaaattaga taaatcgtat ttgtcaatca gagtgctttt ggcgaagaag
     3901 gagaataggg ttggcaccag cagcgcactt tgaatcttgt aatcctgaag gctcctcaga
     3961 aacagctctt cttcaaatct atacattaag acgactcgaa atccacatat caaatatccg
     4021 agtgtagtaa acattccaaa accgtgatgg aatggaacaa cacttaaaat cgcagtatcc
     4081 ggaatgattt gattgccaaa aataggatct ctggcatgcg agaatctcac gcaggcagtt
     4141 ctatgaggca gagcgacacc tttaggcaga ccagtagatc cagaggagtt catgatcagt
     4201 gcaattgtct tgtccctatc gaaggactct ggcacaaaat cgtattcatt aaaaccggga
     4261 ggtagatgag atgtgacgaa cgtgtacatc gactgaaatc cctggtaatc cgttttagaa
     4321 tccatgataa taattttttg gatgattggg agcttttttt gcacgttcaa aattttttgc
     4381 aacccctttt tggaaacgaa caccacggta ggctgcgaaa tgcccatact gttgagcaat
     4441 tcacgttcat tataaatgtc gttcgcgggc gcaactgcaa ctccgataaa taacgcgccc
     4501 aacaccggca taaagaattg aagagagttt tcactgcata cgacgattct gtgatttgta
     4561 ttcagcccat atcgtttcat agcttctgcc aaccgaacgg acatttcgaa gtactcagcg
     4621 taagtgatgt ccacctcgat atgtgcatct gtaaaagcaa ttgttccagg aaccagggcg
     4681 tatctcttca tagccttatg cagttgctct ccagcggttc catcttccag cggatagaat
     4741 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc aacagtaccg
     4801 gaatgccaag cttagtggat caacgttagc cagcttgaag ttcctatact ttctagagaa
     4861 taggaacttc ggaatagtaa cttcaatgat catcatgaca gatctgcgcg cgatcgataa
     4921 atgtttgacc tgcagagctt atgggatagc ttggctttac ccaaagacat tacccctctg
     4981 tgagggccag aggcgggagg taaaggcacg agggggtcca aaaccagatc cccagactat
     5041 caagtcaccc cagtttctta ttgccaaacc acaccaagaa aaccagagtt agactcaaac
     5101 aggcttttat tattcacgta ctcggtagaa aacgggggtt tagtaaacag ggtggaactg
     5161 tataggaaga cgctgggttg gtccgatact atttacacgt ggtggcagcg ctgttcgtca
     5221 catcaggcac agcacgtgca cttgtccgcg gcgcctttgc agacacagcc ctgggcacat
     5281 ttggagcagc ccacgggaca gcaggagcag cagcctagaa aggaggacaa gctgtacaag
     5341 taaagcggcc gcgtcgagcc ctatgggtac catgcatgta ttcaatctaa gcaggctttc
     5401 actttctcgc caacttacaa ggcctttctg tgtaaacaat acctgaacct ttaccccgtt
     5461 gcccggcaac ggccaggtct gtgccaagtg tttgctgacg caacccccac tggctggggc
     5521 ttggtcatgg gccatcagcg catgcgtgga accttttcgg ctcctctgcc gatccatact
     5581 gcggaactcc tagccgcttg ttttgctcgc agcaggtctg gagcaaacat tatcgggact
     5641 gataactctg ttgtcctatc ccgcaaatat acatcgtttc catggctgct aggctgtgct
     5701 gccaactgga tcctgcgcgg gacgtccttt gtttacgtcc cgtcggcgct gaatcctgcg
     5761 gacgaccctt ctcggggtcg cttgggactc tctcgtcccc ttctccgtct gccgttccga
     5821 ccgaccacgg ggcgcacctc tctttacgcg gactccccgt ctgtgccttc tcatctgccg
     5881 gaccgtgtgc acttcgcttc acctctgcac gtcgcatgga gaccaccgtg aacgcccacc
     5941 aaatattgcc caaggtctta cataagagga ctcttggact ctcagcaatg tcaacgaccg
     6001 accttgaggc atacttcaaa gactgtttgt ttaaagactg ggaggagttg ggggaggaga
     6061 ttaggttaaa ggtctttgta ctaggaggct gtaggcataa attggtctgc gcaccagcac
     6121 catggcgcaa tcactagagc ggggtacctt taagaccaat gacttacaag gcagctgtag
     6181 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6241 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6301 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6361 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6421 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     6481 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     6541 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6601 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     6661 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6721 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6781 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     6841 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     6901 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     6961 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     7021 cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7081 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7141 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7201 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7261 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7321 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7381 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7441 tttaacgcga attttaacaa aatattaacg tttacaattt cccaggtggc acttttcggg
     7501 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7561 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7621 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7681 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7741 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7801 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7861 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgaga
     7921 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7981 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8041 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8101 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8161 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8221 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8281 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8341 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8401 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8461 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8521 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8581 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8641 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8701 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8761 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     8821 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8881 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     8941 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9001 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9061 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     9421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     9481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     9541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     9601 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     9661 ccctcactaa agggaacaaa agctggagct gcaagctt
//
