LOCUS       V004914                 8218 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004914
VERSION     V004914
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Goncalves MA, Janssen JM, Holkers M, de Vries AA.
  TITLE     Rapid and sensitive lentivirus vector-based conditional gene
            expression assay to monitor and quantify cell fusion activity
  JOURNAL   PLoS ONE 5 (6), E10954 (2010)
   PUBMED   20532169
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   Goncalves MAF.V., Janssen JM, Holkers M, de Vries AAF.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2009) Molecular Cell Biology, Leiden University
            Medical Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
            Netherlands
REFERENCE   3  (bases 1 to 8218)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8218)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2010"; volume: "5"; issue: "6"; pages: "E10954"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-DEC-2009) Molecular Cell Biology, Leiden University Medical
            Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1799..1916
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        1939..2242
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2243..2446
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2581..3876
                     /label="FLPo"
                     /note="nuclear-targeted site-specific recombinase"
     CDS             4201..4641
                     /gene="X"
                     /label="Protein X"
                     /note="Protein X from Hepatitis B virus genotype D subtype
                     ayw (isolate France/Tiollais/1979). Accession#: P03165"
     LTR             4733..4966
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5038..5172
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5199..5334
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5355..5373)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5383..5399)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5544..5999
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6028..6132
                     /label="AmpR promoter"
     CDS             6133..6990
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7164..7752
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8040..8061
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8076..8106
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8114..8130
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8138..8154
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8175..8193
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1861 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     1921 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     1981 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2041 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2101 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2161 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2221 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2281 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2341 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2401 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     2461 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
     2521 atccagcctc cgcggccggg aacggtgcat tggaagcttg gtaccggtga attcgctagc
     2581 atggctccca agaagaagag gaaggtgatg agtcaatttg atatattatg taaaacacca
     2641 cctaaggtcc tggttcgtca gtttgtggaa aggtttgaaa gaccttcagg ggaaaaaata
     2701 gcatcatgtg ctgctgaact aacctattta tgttggatga ttactcataa cggaacagca
     2761 atcaagagag ccacattcat gagctataat actatcataa gcaattcgct gagtttcgat
     2821 attgtcaaca aatcactcca gtttaaatac aagacgcaaa aagcaacaat tctggaagcc
     2881 tcattaaaga aattaattcc tgcttgggaa tttacaatta ttccttacaa tggacaaaaa
     2941 catcaatctg atatcactga tattgtaagt agtttgcaat tacagttcga atcatcggaa
     3001 gaagcagata agggaaatag ccacagtaaa aaaatgctta aagcacttct aagtgagggt
     3061 gaaagcatct gggagatcac tgagaaaata ctaaattcgt ttgagtatac ctcgagattt
     3121 acaaaaacaa aaactttata ccaattcctc ttcctagcta ctttcatcaa ttgtggaaga
     3181 ttcagcgata ttaagaacgt tgatccgaaa tcatttaaat tagtccaaaa taagtatctg
     3241 ggagtaataa tccagtgttt agtgacagag acaaagacaa gcgttagtag gcacatatac
     3301 ttctttagcg caaggggtag gatcgatcca cttgtatatt tggatgaatt tttgaggaat
     3361 tctgaaccag tcctaaaacg agtaaatagg accggcaatt cttcaagcaa caaacaggaa
     3421 taccaattat taaaagataa cttagtcaga tcgtacaaca aggctttgaa gaaaaatgcg
     3481 ccttatccaa tctttgctat aaagaatggc ccaaaatctc acattggaag acatttgatg
     3541 acctcatttc tgtcaatgaa gggcctaacg gagttgacta atgttgtggg aaattggagc
     3601 gataagcgtg cttctgccgt ggccaggaca acgtatactc atcagataac agcaatacct
     3661 gatcactact tcgcactagt ttctcggtac tatgcatatg atccaatatc aaaggaaatg
     3721 atagcattga aggatgagac taatccaatt gaggagtggc agcatataga acagctaaag
     3781 ggtagtgctg aaggaagcat acgatacccc gcatggaatg ggataatatc acaggaggta
     3841 ctagactacc tttcatccta cataaataga cgcatataat gatctagcta gcgtttgtat
     3901 tcaatctaag caggctttca ctttctcgcc aacttacaag gcctttctgt gtaaacaata
     3961 cctgaacctt taccccgttg cccggcaacg gccaggtctg tgccaagtgt ttgctgacgc
     4021 aacccccact ggctggggct tggtcatggg ccatcagcgc atgcgtggaa ccttttcggc
     4081 tcctctgccg atccatactg cggaactcct agccgcttgt tttgctcgca gcaggtctgg
     4141 agcaaacatt atcgggactg ataactctgt tgtcctatcc cgcaaatata catcgtttcc
     4201 atggctgcta ggctgtgctg ccaactggat cctgcgcggg acgtcctttg tttacgtccc
     4261 gtcggcgctg aatcctgcgg acgacccttc tcggggtcgc ttgggactct ctcgtcccct
     4321 tctccgtctg ccgttccgac cgaccacggg gcgcacctct ctttacgcgg actccccgtc
     4381 tgtgccttct catctgccgg accgtgtgca cttcgcttca cctctgcacg tcgcatggag
     4441 accaccgtga acgcccacca aatattgccc aaggtcttac ataagaggac tcttggactc
     4501 tcagcaatgt caacgaccga ccttgaggca tacttcaaag actgtttgtt taaagactgg
     4561 gaggagttgg gggaggagat taggttaaag gtctttgtac taggaggctg taggcataaa
     4621 ttggtctgcg caccagcacc atggcgcaat cactagagcg gggtaccttt aagaccaatg
     4681 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     4741 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     4801 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4861 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4921 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     4981 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     5041 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5101 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     5161 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     5221 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     5281 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     5341 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     5401 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     5461 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     5521 cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5581 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5641 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5701 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5761 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5821 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5881 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5941 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc
     6001 ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6061 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     6121 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     6181 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     6241 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     6301 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6361 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6421 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6481 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6541 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6601 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6661 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6721 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6781 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6841 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6901 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6961 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     7021 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7081 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7141 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7201 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7261 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     7321 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7381 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7441 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7501 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7561 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7621 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7681 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7741 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7801 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     7861 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     7921 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     7981 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     8041 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     8101 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     8161 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagctt
//
