LOCUS       V004913                 9650 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004913
VERSION     V004913
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Goncalves MA, Janssen JM, Holkers M, de Vries AA.
  TITLE     Rapid and sensitive lentivirus vector-based conditional gene
            expression assay to monitor and quantify cell fusion activity
  JOURNAL   PLoS ONE 5 (6), E10954 (2010)
   PUBMED   20532169
REFERENCE   2  (bases 1 to 9650)
  AUTHORS   Goncalves MAF.V., Janssen JM, Holkers M, de Vries AAF.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2009) Molecular Cell Biology, Leiden University
            Medical Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
            Netherlands
REFERENCE   3  (bases 1 to 9650)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9650)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2010"; volume: "5"; issue: "6"; pages: "E10954"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-DEC-2009) Molecular Cell Biology, Leiden University Medical
            Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    454..579
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1072..1305
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1489..1533
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1682..1723
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1795..1912
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        1935..2238
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2239..2442
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2577..3872
                     /label="FLPo"
                     /note="nuclear-targeted site-specific recombinase"
     misc_feature    4069..4646
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4674..5270
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     CDS             5633..6073
                     /gene="X"
                     /label="Protein X"
                     /note="Protein X from Hepatitis B virus genotype D subtype
                     ayw (isolate France/Tiollais/1979). Accession#: P03165"
     LTR             6165..6398
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6470..6604
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6631..6766
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6787..6805)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6815..6831)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6976..7431
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7460..7564
                     /label="AmpR promoter"
     CDS             7565..8422
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8596..9184
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9472..9493
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9508..9538
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9546..9562
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9570..9586
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9607..9625
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggacc tgaaagcgaa agggaaacca gagctctctc gacgcaggac tcggcttgct
      481 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
      541 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
      601 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
      661 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
      721 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
      781 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
      841 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
      901 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
      961 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     1021 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     1081 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcctcaa tgacgctgac
     1141 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     1201 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     1261 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     1321 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     1381 tctggaacag attggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1441 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1501 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1561 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1621 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     1681 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     1741 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttttaa
     1801 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     1861 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgataa
     1921 gcttgggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     1981 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     2041 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     2101 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     2161 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     2221 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     2281 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     2341 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     2401 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     2461 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
     2521 agcctccgcg gccgggaacg gtgcattgga agcttggtac cggtgaattc gctagcatgg
     2581 ctcccaagaa gaagaggaag gtgatgagtc aatttgatat attatgtaaa acaccaccta
     2641 aggtcctggt tcgtcagttt gtggaaaggt ttgaaagacc ttcaggggaa aaaatagcat
     2701 catgtgctgc tgaactaacc tatttatgtt ggatgattac tcataacgga acagcaatca
     2761 agagagccac attcatgagc tataatacta tcataagcaa ttcgctgagt ttcgatattg
     2821 tcaacaaatc actccagttt aaatacaaga cgcaaaaagc aacaattctg gaagcctcat
     2881 taaagaaatt aattcctgct tgggaattta caattattcc ttacaatgga caaaaacatc
     2941 aatctgatat cactgatatt gtaagtagtt tgcaattaca gttcgaatca tcggaagaag
     3001 cagataaggg aaatagccac agtaaaaaaa tgcttaaagc acttctaagt gagggtgaaa
     3061 gcatctggga gatcactgag aaaatactaa attcgtttga gtatacctcg agatttacaa
     3121 aaacaaaaac tttataccaa ttcctcttcc tagctacttt catcaattgt ggaagattca
     3181 gcgatattaa gaacgttgat ccgaaatcat ttaaattagt ccaaaataag tatctgggag
     3241 taataatcca gtgtttagtg acagagacaa agacaagcgt tagtaggcac atatacttct
     3301 ttagcgcaag gggtaggatc gatccacttg tatatttgga tgaatttttg aggaattctg
     3361 aaccagtcct aaaacgagta aataggaccg gcaattcttc aagcaacaaa caggaatacc
     3421 aattattaaa agataactta gtcagatcgt acaacaaggc tttgaagaaa aatgcgcctt
     3481 atccaatctt tgctataaag aatggcccaa aatctcacat tggaagacat ttgatgacct
     3541 catttctgtc aatgaagggc ctaacggagt tgactaatgt tgtgggaaat tggagcgata
     3601 agcgtgcttc tgccgtggcc aggacaacgt atactcatca gataacagca atacctgatc
     3661 actacttcgc actagtttct cggtactatg catatgatcc aatatcaaag gaaatgatag
     3721 cattgaagga tgagactaat ccaattgagg agtggcagca tatagaacag ctaaagggta
     3781 gtgctgaagg aagcatacga taccccgcat ggaatgggat aatatcacag gaggtactag
     3841 actacctttc atcctacata aatagacgca tataatgatc tagctagcgt taacggatcc
     3901 tctagcctag tgccatttgt tcagtggttc gtagggcttt cccccactgt ttggctttca
     3961 gttatatgga tgatgtggta ttgggggcca agtctgtaca gcatcttgag tcccttttta
     4021 ccgctgttac caattttctt ttgtctttgg gtaaacccgg gaattccgcc cctctccctc
     4081 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     4141 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     4201 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     4261 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     4321 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     4381 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     4441 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     4501 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     4561 atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     4621 ctttgaaaaa cacgatgata atatgggata atatggccac aacggtcgcc accatgaccg
     4681 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     4741 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     4801 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     4861 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     4921 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     4981 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     5041 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     5101 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     5161 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     5221 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     5281 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatgt attcaatcta
     5341 agcaggcttt cactttctcg ccaacttaca aggcctttct gtgtaaacaa tacctgaacc
     5401 tttaccccgt tgcccggcaa cggccacctc tgtgccaagt gtttgctgac gcaaccccca
     5461 ctggctgggg cttggtcatg ggccatcagc gcatgcgtgg aaccttttcg gctcctctgc
     5521 cgatccatac tgcggaactc ctagccgctt gttttgctcg cagcaggtct ggagcaaaca
     5581 ttatcgggac tgataactct gttgtcctat cccgcaaata tacatcgttt ccatggctgc
     5641 taggctgtgc tgccaactgg atcctgcgcg ggacgtcctt tgtttacgtc ccgtcggcgc
     5701 tgaatcctgc ggacgaccct tctcggggtc gcttgggact ctctcgtccc cttctccgtc
     5761 tgccgttccg accgaccacg gggcgcacct ctctttacgc ggactccccg tctgtgcctt
     5821 ctcatctgcc ggaccgtgtg cacttcgctt cacctctgca cgtcgcatgg agaccaccgt
     5881 gaacgcccac caaatattgc ccaaggtctt acataagagg actcttggac tctcagcaat
     5941 gtcaacgacc gaccttgagg catacttcaa agactgtttg tttaaagact gggaggagtt
     6001 gggggaggag attaggttaa aggtctttgt actaggaggc tgtaggcata aattggtctg
     6061 cgcaccagca ccatggcgca atcactagag cggggtacct ttaagaccaa tgacttacaa
     6121 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     6181 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     6241 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     6421 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     6481 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6541 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6601 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6661 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     6721 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     6781 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     6841 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     6901 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     6961 atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     7021 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     7081 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7141 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     7201 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7261 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7321 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7381 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcccaggtgg
     7441 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7501 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7561 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7621 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7681 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7741 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7801 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7861 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7921 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7981 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8041 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8101 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8161 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8221 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8281 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8341 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8401 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8461 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8521 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8581 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8641 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8701 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     8761 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8821 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8881 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8941 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9001 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9061 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9121 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9181 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9241 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9301 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9361 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9421 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     9481 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     9541 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     9601 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
//
